CRISPR/Cas9 system-based myostatin-targeted disruption promotes somatic growth and adipogenesis in loach, Misgurnus anguillicaudatus

Tao, Binbin; Tan, Juan; Chen, Lu; Xu, Yuanfeng; Liao, Xianyao; Li, Yongming; Chen, Ji; Song, Yanlong; Hu, Wei

doi:10.1016/j.aquaculture.2021.737097

Cited by 16 publications

(6 citation statements)

References 48 publications

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“…Interestingly, the authors reported an increase of fat accumulation in muscle of mstnb -/- zebrafish and showed evidences for a transition of energy supply from an amino acid- to a lipid-dependent source ( 150 ). Recently, CRISPR/Cas9 inactivation of mstn in loach ( Misgurnus anguillicaudatus ) led to an increase of fibers number but also accumulation of lipids, especially in red muscle ( 195 ).…”

Section: Myokinesmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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Control of tissue metabolism and growth involves interactions between organs, tissues, and cell types, mediated by cytokines or direct communication through cellular exchanges. Indeed, over the past decades, many peptides produced by adipose tissue, skeletal muscle and bone named adipokines, myokines and osteokines respectively, have been identified in mammals playing key roles in organ/tissue development and function. Some of them are released into the circulation acting as classical hormones, but they can also act locally showing autocrine/paracrine effects. In recent years, some of these cytokines have been identified in fish models of biomedical or agronomic interest. In this review, we will present their state of the art focusing on local actions and inter-tissue effects. Adipokines reported in fish adipocytes include adiponectin and leptin among others. We will focus on their structure characteristics, gene expression, receptors, and effects, in the adipose tissue itself, mainly regulating cell differentiation and metabolism, but in muscle and bone as target tissues too. Moreover, lipid metabolites, named lipokines, can also act as signaling molecules regulating metabolic homeostasis. Regarding myokines, the best documented in fish are myostatin and the insulin-like growth factors. This review summarizes their characteristics at a molecular level, and describes both, autocrine effects and interactions with adipose tissue and bone. Nonetheless, our understanding of the functions and mechanisms of action of many of these cytokines is still largely incomplete in fish, especially concerning osteokines (i.e., osteocalcin), whose potential cross talking roles remain to be elucidated. Furthermore, by using selective breeding or genetic tools, the formation of a specific tissue can be altered, highlighting the consequences on other tissues, and allowing the identification of communication signals. The specific effects of identified cytokines validated through in vitro models or in vivo trials will be described. Moreover, future scientific fronts (i.e., exosomes) and tools (i.e., co-cultures, organoids) for a better understanding of inter-organ crosstalk in fish will also be presented. As a final consideration, further identification of molecules involved in inter-tissue communication will open new avenues of knowledge in the control of fish homeostasis, as well as possible strategies to be applied in aquaculture or biomedicine.

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Section: Myokinesmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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“…Mutation of mstn in L. crocea Among numbers of species, it was clear that mstnb played a signi cant role in negatively regulating growth. Mstnb-de cient zebra sh and loach (Misgurnus anguillicaudatus) showed signi cantly increasing muscle mass with muscle ber hyperplasia (Gao et al, 2016, Tao et al, 2021, and the body weight and body length of the mstnab +/− common carp were signi cantly enhanced (Shahi et al, 2022), which provided a promising direction in aquaculture output of economic sh. Therefore, we selected mstnb of the large yellow croaker as the target gene.…”

Section: Resultsmentioning

confidence: 99%

“…2c). It was likely that different from single gRNA microinjection (Shahi et al, 2022, Tao et al, 2021, Zhang et al, 2020b, the injection of multiple gRNAs simultaneously could induce larger segments deletion between two target sites (Fig. 2c).…”

Section: Resultsmentioning

confidence: 99%

Disruption of mstn Gene by CRISPR/Cas9 in Large Yellow Croaker (Larimichthys crocea)

Yan

Wang

et al. 2022

Mar Biotechnol

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The large yellow croaker (Larimichthys crocea) plays an economically vital role in the marine aquaculture in China. Suffering from infection of bacteria and protozoon, effect of extreme weather and stress from high density farming, genome editing is thought to be an important tool applied to L. croea for enhancing commercial traits such as growth rate, disease resistance and nutrition component. In this study, we identi ed two mstn genes in L. croea and investigated the different phylogenetic clades, gene structures, and conserved syntenic relationships. To obtain fast-growing large yellow croaker, we specially selected two validated targets for mstnb knockout, which was homologous to mammalian myostatin gene (MSTN) and down-regulated skeletal muscle growth and development. Five signi cant mutation types were generated in two mosaic mutants by transferring speci c CRISPR/Cas9 RNPs (ribonucleoprotein) into the one-cell fertilized embryos based on CRISPR/Cas9 technology. Subsequently, we also elucidated the obstacles and possible measures to improve the success rate of inducing modi ed large yellow croaker. Our results would provide valuable method and reference for facilitating genome editing programs of the large yellow croaker in the future.

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“…compound and cryosectioned. As described previously ( 38 ), the frozen sections were stained with Oil Red O to visualize fat deposits in the liver. The images were observed and captured by a microscope with a CCD (Aperio Versa 8 Scanner, Leica, Germany), then the relative area of lipid droplet was analyzed using Image-Pro Plus 6.0.…”

Section: Methodsmentioning

confidence: 99%

Improved liver lipid catabolism and utilization in growth hormone transgenic common carp (Cyprinus carpio L.) through enhanced lipolytic and fatty acid β-oxidation pathways

et al. 2022

Front. Endocrinol.

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Growth hormone (GH) transgenic common carp (Cyprinus carpio L.) show desirable aquaculture traits. Their specific growth rate (SGR) and feed efficiency (FE) are approximately 12% and 17% higher than the wild-type (WT) common carp, respectively. However, the mechanisms of lipid catabolism (lipolysis and fatty acid β-oxidation) and utilization in GH transgenic common carp are still unclear. In this study, we firstly compared the lipid metabolism of GH transgenic (initial weight 3.72 ± 0.32 g) and WT (initial weight 3.30 ± 0.28 g) common carp fed with a normal fat level diet (6% lipid, 33% protein) for two months, then compared the growth performance of GH transgenic (initial weight 3.65 ± 0.33 g) and WT (initial weight 3.27 ± 0.26 g) common carp fed with different fat levels diets (6% lipid and 12% lipid, 33% protein) for two months. We found that the lipid content in serum, liver and whole body was significantly reduced in GH transgenic common carp, the hepatic activities of the lipolytic enzymes hormone-sensitive lipase and adipose triglyceride lipase were enhanced, and the hepatic expression level of hormone-sensitive lipase was upregulated. In addition, the mitochondrion numbers were increased, and the expression level of carnitine palmitoyltransferase-1a and carnitine palmitoyltransferase-1b was upregulated in the liver of GH transgenic common carp. GH transgenic common carp showed higher weight gain and SGR than that in WT carp when fed with a normal-fat diet as they did when fed with a high-fat diet, and GH transgenic common carp showed higher FE than that in WT carp when fed with a high-fat diet. These results suggested that the lipid catabolism and utilization was improved in the GH transgenic common carp liver through enhanced lipolytic and fatty acid β-oxidation pathways. Our study provides new insights into improving lipid utilization in some aquaculture fish species.

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CRISPR/Cas9 system-based myostatin-targeted disruption promotes somatic growth and adipogenesis in loach, Misgurnus anguillicaudatus

Cited by 16 publications

References 48 publications

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Disruption of mstn Gene by CRISPR/Cas9 in Large Yellow Croaker (Larimichthys crocea)

Improved liver lipid catabolism and utilization in growth hormone transgenic common carp (Cyprinus carpio L.) through enhanced lipolytic and fatty acid β-oxidation pathways

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