CRISPR/Cas9-mediated SERS/colorimetric dual-mode lateral flow platform combined with smartphone for rapid and sensitive detection of Staphylococcus aureus

Wang, Junfeng; Jiang, Han; Chen, Yuhong; Zhu, Xiaofan; Wu, Qian; Chen, Wei; Zhao, Qihong; Wang, Jie; Qin, Panzhu

doi:10.1016/j.bios.2024.116046

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…77 aureus (Figure 3C). 64 Unlike the trans-cleavage of Cas12a, CRISPR Cas9-single guide RNA recognition binds to dsDNA (amplification product) and performs double-stranded cutting.…”

Section: Surface-enhanced Raman Scattering (Sers) Basedmentioning

confidence: 99%

“…The LOD of S. aureus was 1 CFU/mL within 1 h. Wang et al. constructed a dual mode recombinase lateral flow strips based on CRISPR Cas9 assisted with polymerase amplification for monitoring S. aureus (Figure C) . Unlike the trans-cleavage of Cas12a, CRISPR Cas9-single guide RNA recognition binds to dsDNA (amplification product) and performs double-stranded cutting.…”

Section: Optical Biosensorsmentioning

confidence: 99%

“…fabricated cross-priming amplification Cas12a-LFS for detection MRSA with the LOD of 5 CFU/mL . Interestingly, considering its portability, user friendliness, and ease of operation, multiple research groups coincidentally chose CRISPR Cas-lateral flow strips method. ,− This detection process usually takes 30–70 min and is very suitable for rapid on-site testing.…”

Section: Optical Biosensorsmentioning

confidence: 99%

“…(B) A colorimetric biosensor based on bacteriophage coupled nanoenzyme for S. aureus detection 63. (C) A SERS and colorimetric dual mode lateral flow biosensor for S. aureus detection 64.…”

mentioning

confidence: 99%

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Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Wang,

Yang

2024

J. Agric. Food Chem.

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Staphylococcus aureus (S. aureus) is a kind of pathogenic bacteria which can lead to food poisoning, hospital, and community infections. S. aureus and methicillin-resistant S. aureus (MRSA) have become headaches for public health worldwide. Therefore, strengthening the detection of S. aureus and MRSA is a critical step to prevent and control its spread and infection. This review summarized multiple detection methods (electrochemical, optical, and other biosensors) for sensitive and efficient detection of nonresistant and resistant S. aureus. First, we have introduced the principle and methods of detection platform for S. aureus and MRSA. We also contrasted various detection strategies. Finally, the current situation and prospect of S. aureus and MRSA detection in the future are explored in depth, and its development direction of detection methods is also predicted. In this review, we found that although biosensors have shown tremendous brilliance in the field of monitoring, they are currently in the experimental stage. It can be certain that we are very close to entering the commercialization stage. The point-of care testing available to nonprofessionals will become a new direction. We firmly believe that the monitoring system will be more perfect and stable and public life will be healthier and safer.

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“…77 aureus (Figure 3C). 64 Unlike the trans-cleavage of Cas12a, CRISPR Cas9-single guide RNA recognition binds to dsDNA (amplification product) and performs double-stranded cutting.…”

Section: Surface-enhanced Raman Scattering (Sers) Basedmentioning

confidence: 99%

Section: Optical Biosensorsmentioning

confidence: 99%

Section: Optical Biosensorsmentioning

confidence: 99%

“…(B) A colorimetric biosensor based on bacteriophage coupled nanoenzyme for S. aureus detection 63. (C) A SERS and colorimetric dual mode lateral flow biosensor for S. aureus detection 64.…”

mentioning

confidence: 99%

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Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Wang,

Yang

2024

J. Agric. Food Chem.

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“…134 Similar to fluorescence sensing, SERS can also be used to obtain recognizable signals through a smartphone-integrated portable Raman spectrometer. 135…”

Section: Recent Development Of Multimodal Sensors Based On Mnp Detectionmentioning

confidence: 99%

Multimodal biosensing systems based on metal nanoparticles

Yan,

Zheng,

Wang

et al. 2024

Analyst

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Accurate and simultaneous detection of pork and horse meat adulteration by double tailed recombinase polymerase amplification integrated with SERS based two-color lateral flow nucleic acid hybridization strip

Liu,

Cao,

Wang

et al. 2024

Journal of Food Composition and Analysis

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CRISPR/Cas9-mediated SERS/colorimetric dual-mode lateral flow platform combined with smartphone for rapid and sensitive detection of Staphylococcus aureus

Cited by 6 publications

References 28 publications

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Multimodal biosensing systems based on metal nanoparticles

Accurate and simultaneous detection of pork and horse meat adulteration by double tailed recombinase polymerase amplification integrated with SERS based two-color lateral flow nucleic acid hybridization strip

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