CRISPR/Cas9-mediated mutagenesis of sweet basil candidate susceptibility gene ObDMR6 enhances downy mildew resistance

Hasley, Jeremieh Abram R.; Navet, Natasha; Tian, Mingxing

doi:10.1371/journal.pone.0253245

Cited by 46 publications

(32 citation statements)

References 42 publications

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“… The grapevine DMR6 and DLO protein family. ( A ) Multiple alignment of grapevine DMR6 and DLO proteins with Zea mays ZmFNSI (NP_001151167) [ 40 ], Hordeum vulgare HvDMR6 (KAE8782493.1) [ 41 ], Solanum lycopersicon SlDMR6-1 (Solyc03g080190) and SlDMR6-2 (Solyc06g073080) [ 13 ], Solanum tuberosum StDMR6 (XP_006347521) [ 15 ], Ocimum basilicum ObDMR6 (QWT44767.1) [ 17 ], Musa acuminata MusaDMR6 (XP_009389864.1) [ 16 ], Arabidopsis thaliana AtDLO1 (At4g10500), AtDLO2 (At4g10490), and AtDMR6 (AT5G24530) [ 11 ]. The alignment is coloured with the default ClustalX colour scheme according to the amino acid chemical properties.…”

Section: Figurementioning

confidence: 99%

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Grapevine DMR6-1 Is a Candidate Gene for Susceptibility to Downy Mildew

et al. 2022

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Grapevine (Vitis vinifera) is a valuable crop in Europe for both economical and cultural reasons, but highly susceptible to Downy mildew (DM). The generation of resistant vines is of critical importance for a sustainable viticulture and can be achieved either by introgression of resistance genes in susceptible varieties or by mutation of Susceptibility (S) genes, e.g., by gene editing. This second approach offers several advantages: it maintains the genetic identity of cultivars otherwise disrupted by crossing and generally results in a broad-spectrum and durable resistance, but it is hindered by the poor knowledge about S genes in grapevines. Candidate S genes are Downy mildew Resistance 6 (DMR6) and DMR6-Like Oxygenases (DLOs), whose mutations confer resistance to DM in Arabidopsis. In this work, we show that grapevine VviDMR6-1 complements the Arabidopsis dmr6-1 resistant mutant. We studied the expression of grapevine VviDMR6 and VviDLO genes in different organs and in response to the DM causative agent Plasmopara viticola. Through an automated evaluation of causal relationships among genes, we show that VviDMR6-1, VviDMR6-2, and VviDLO1 group into different co-regulatory networks, suggesting distinct functions, and that mostly VviDMR6-1 is connected with pathogenesis-responsive genes. Therefore, VviDMR6-1 represents a good candidate to produce resistant cultivars with a gene-editing approach.

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Section: Figurementioning

confidence: 99%

“…Together, these results suggest different functions of DMR6 homologs. In addition, recent works proved the role of DMR6 in susceptibility to Xanthomonas wilt in banana [ 16 ] and to Peronospora belbahrii in sweet basil [ 17 ], suggesting a widely conserved function across the plant kingdom.…”

Section: Introductionmentioning

confidence: 99%

Grapevine DMR6-1 Is a Candidate Gene for Susceptibility to Downy Mildew

et al. 2022

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“…This will ultimately result in the development of pea genotypes having wider PM resistance for more number of pathogen races [ 132 ]. Recently, the genome editing has been undertaken for the Mlo locus to achieve the resistance in different crops including wheat [ 138 ], grapevine [ 139 ], tomato [ 140 ] and sweet basil [ 141 ].…”

Section: Durable Resistance Strategies For Pm Resistancementioning

confidence: 99%

Gene-Based Resistance to Erysiphe Species Causing Powdery Mildew Disease in Peas (Pisum sativum L.)

Devi

Mishra

Sagar

et al. 2022

Genes

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Globally powdery mildew (PM) is one of the major diseases of the pea caused by Erysiphe pisi. Besides, two other species viz. Erysiphe trifolii and Erysiphe baeumleri have also been identified to infect the pea plant. To date, three resistant genes, namely er1, er2 and Er3 located on linkage groups VI, III and IV respectively were identified. Studies have shown the er1 gene to be a Pisum sativum Mildew resistance Locus ‘O’ homologue and subsequent analysis has identified eleven alleles namely er1–1 to er1–11. Despite reports mentioning the breakdown of er1 gene-mediated PM resistance by E. pisi and E. trifolii, it is still the most widely deployed gene in PM resistance breeding programmes across the world. Several linked DNA markers have been reported in different mapping populations with varying linkage distances and effectiveness, which were used by breeders to develop PM-resistant pea cultivars through marker assisted selection. This review summarizes the genetics of PM resistance and its mechanism, allelic variations of the er gene, marker linkage and future strategies to exploit this information for targeted PM resistance breeding in Pisum.

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“…Improving resistance to citrus canker has been an important citrus breeding objective (Gmitter and Talon, 2008). Recent studies have shown that disabling disease susceptibility ( S ) genes including DOWNY MILDEW RESISTANCE 6 ( DMR6 ) can be a promising approach to engineering durable and broad-spectrum resistance to bacterial pathogens (de Toledo Thomazella, et al ., 2021; Hasley, et al ., 2021; Kieu, et al ., 2021; Tripathi, et al ., 2021).…”

Section: Figurementioning

confidence: 99%

Editing the CsDMR6 Gene in Citrus Results in Resistance to the Bacterial Disease Citrus Canker

Parajuli

Huo

Gmitter

et al. 2022

Preprint

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Citrus is one of the most important fruit crops in the world. Citrus production worldwide faces challenges from devastating bacterial diseases, including citrus canker caused by Xanthomonas citri ssp. citri (Xcc). Improving citrus resistance to citrus canker and other major bacterial diseases has been a top priority in citrus biotechnology. Disabling disease susceptibility genes has emerged as a novel, promising approach to engineering disease resistance. The bottleneck for applying such an approach has been the identification of proper disease susceptibility-related genes in citrus. Here we show the first successful case of editing the CsDMR6 gene in citrus and obtaining strong resistance to citrus canker in six mutants in two citrus cultivars, Duncan grapefruit and Carrizo citrange. Multiple types of deletions and insertions were induced in CsDMR6, resulting in frameshift of its coding region and presumably loss of gene function. The mutation frequency in most of the mutants reached 71.8% to 98.9%. The mutants showed 71.2% to 99.8% reduction in citrus canker lesion and greater than 99.7% or 2.45 to 4.95 Log10 unit reduction in Xcc bacterial cell population. Mutants also accumulated more salicylic acid and expressed much higher levels of the NPR1 gene than the wildtype with or without Xcc inoculation, which suggests potential resistance to other diseases in these mutants. The guide RNAs for targeting CsDMR6 were selected from highly conserved regions and have 100% nucleotide identity with DMR6 homologs in important citrus species; these guide RNAs are expected to work in other important citrus species and cultivars.

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CRISPR/Cas9-mediated mutagenesis of sweet basil candidate susceptibility gene ObDMR6 enhances downy mildew resistance

Cited by 46 publications

References 42 publications

Grapevine DMR6-1 Is a Candidate Gene for Susceptibility to Downy Mildew

Grapevine DMR6-1 Is a Candidate Gene for Susceptibility to Downy Mildew

Gene-Based Resistance to Erysiphe Species Causing Powdery Mildew Disease in Peas (Pisum sativum L.)

Editing the CsDMR6 Gene in Citrus Results in Resistance to the Bacterial Disease Citrus Canker

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