CRISPR/Cas9-mediated mutagenesis of Clpsk1 in watermelon to confer resistance to Fusarium oxysporum f.sp. niveum

Zhang, Man; Liu, Qiling; Yang, Xingping; Xu, Jianfei; Liu, Guang; Yao, Xiefeng; Ren, Runsheng; Xu, Jian; Lou, Laiqing

doi:10.1007/s00299-020-02516-0

Cited by 80 publications

(27 citation statements)

References 41 publications

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“…Similarly, knockout of the psk1 gene in C. lanatus conferred resistance to Fusarium oxysporum f.sp. niveum , a prominent fungal pathogen of the watermelon to which few cultivated varieties are resistant Zhang et al (2020) . In cacao, an analogous result was achieved by knocking out the NPR3 gene for increased resistance to fungal pathogens ( Fister et al, 2018 ).…”

Section: Applications Of Gene Editing In Orphan Cropsmentioning

confidence: 99%

Current Advancements and Limitations of Gene Editing in Orphan Crops

Venezia

Krainer

2021

Front. Plant Sci.

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Gene editing provides precise, heritable genome mutagenesis without permanent transgenesis, and has been widely demonstrated and applied in planta. In the past decade, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) has revolutionized the application of gene editing in crops, with mechanistic advances expanding its potential, including prime editing and base editing. To date, CRISPR/Cas has been utilized in over a dozen orphan crops with diverse genetic backgrounds, leading to novel alleles and beneficial phenotypes for breeders, growers, and consumers. In conjunction with the adoption of science-based regulatory practices, there is potential for CRISPR/Cas-mediated gene editing in orphan crop improvement programs to solve a plethora of agricultural problems, especially impacting developing countries. Genome sequencing has progressed, becoming more affordable and applicable to orphan crops. Open-access resources allow for target gene identification and guide RNA (gRNA) design and evaluation, with modular cloning systems and enzyme screening methods providing experimental feasibility. While the genomic and mechanistic limitations are being overcome, crop transformation and regeneration continue to be the bottleneck for gene editing applications. International collaboration between all stakeholders involved in crop improvement is vital to provide equitable access and bridge the scientific gap between the world’s most economically important crops and the most under-researched crops. This review describes the mechanisms and workflow of CRISPR/Cas in planta and addresses the challenges, current applications, and future prospects in orphan crops.

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Section: Applications Of Gene Editing In Orphan Cropsmentioning

confidence: 99%

Current Advancements and Limitations of Gene Editing in Orphan Crops

Venezia

Krainer

2021

Front. Plant Sci.

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“…niveum (FON). The loss-of-function mutation of ClPSK1 rendered watermelon seedlings more resistant to infection by FON (Zhang et al, 2020b). Recently, it was reported that the ClWIP1, a homologue of CsWIP1 and CmWIP1 in cucumber and melon, respectively, acts as the gynoecious (gy) gene in watermelon (Martin et al, 2009).…”

Section: Watermelonmentioning

confidence: 99%

Applications and Major Achievements of Genome Editing in Vegetable Crops: A Review

Kim

Kang²,

Yang

et al. 2021

Front. Plant Sci.

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The emergence of genome-editing technology has allowed manipulation of DNA sequences in genomes to precisely remove or replace specific sequences in organisms resulting in targeted mutations. In plants, genome editing is an attractive method to alter gene functions to generate improved crop varieties. Genome editing is thought to be simple to use and has a lower risk of off-target effects compared to classical mutation breeding. Furthermore, genome-editing technology tools can also be applied directly to crops that contain complex genomes and/or are not easily bred using traditional methods. Currently, highly versatile genome-editing tools for precise and predictable editing of almost any locus in the plant genome make it possible to extend the range of application, including functional genomics research and molecular crop breeding. Vegetables are essential nutrient sources for humans and provide vitamins, minerals, and fiber to diets, thereby contributing to human health. In this review, we provide an overview of the brief history of genome-editing technologies and the components of genome-editing tool boxes, and illustrate basic modes of operation in representative systems. We describe the current and potential practical application of genome editing for the development of improved nutritious vegetables and present several case studies demonstrating the potential of the technology. Finally, we highlight future directions and challenges in applying genome-editing systems to vegetable crops for research and product development.

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“…tomato DC3000 Cas9 Ortigosa et al., 2018 SlDMR6 P. syringae , Phytophthora capsici, Xanthomonas spp. Cas9 de Toledo Thomazella et al., 2016 SlPMR4 Oidium neolycopersici (powdery mildew) Cas9 Santillán Martínez et al., 2020 Watermelon Clpsk1 Fusarium oxysporum Cas9 Zhang et al., 2020 Wheat TaMLO Blumeria graminis (powdery mildew) Cas9 Wang et al., 2014 TaEDR1 Blumeria graminis Cas9 Zhang et al., 2017b TaNFXL1 Fusarium graminearum (head blight) Cas0 Brauer et al., 2020 …”

Section: Approaches To Engineer Disease Resistancementioning

confidence: 99%

Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors

Schenke

Cai

2020

iScience

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Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability of crop and pathogen genomic information, as well as emerging genome editing permits a novel approach for breeding of crop disease resistance. We describe here strategies to identify new targets for resistance breeding with focus on interruption of the compatible plant-pathogen interaction by CRISPR/ Cas-mediated genome editing. Basically, crop genome editing can be applied in several ways to achieve this goal. The most common approach focuses on the ''simple'' knockout by non-homologous end joining repair of plant susceptibility factors required for efficient host colonization. However, genome rewriting via homology-directed repair or base editing can also prevent host manipulation by changing the targets of pathogen-derived effectors or molecules beyond recognition, which also decreases plant susceptibility. We conclude that genome editing by CRISPR/Cas will become increasingly indispensable to generate in relatively short time beneficial resistance traits in crops to meet upcoming challenges.

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CRISPR/Cas9-mediated mutagenesis of Clpsk1 in watermelon to confer resistance to Fusarium oxysporum f.sp. niveum

Cited by 80 publications

References 41 publications

Current Advancements and Limitations of Gene Editing in Orphan Crops

Current Advancements and Limitations of Gene Editing in Orphan Crops

Applications and Major Achievements of Genome Editing in Vegetable Crops: A Review

Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors

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