CRISPR/Cas9-Mediated Mutagenesis of Carotenoid Cleavage Dioxygenase 8 (CCD8) in Tobacco Affects Shoot and Root Architecture

Gao, Junping; Zhang, Tong; Xu, Bin; Lee, Jia; Xiao, Bingguang; Liu, He; Liu, Lijing; Yan, Hao; Xia, Qingyou

doi:10.3390/ijms19041062

Cited by 43 publications

(32 citation statements)

References 97 publications

(157 reference statements)

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“…Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway [ 82 ] and have been widely implicated in acclimation to environmental stresses [ 83 , 84 ]. Recent studies suggest that CRISPR/Cas9-mediated mutagenesis of CCD8 alters the root and shoot architecture and provides resistance against some parasitic weeds [ 85 , 86 , 87 ]. In our study, CCD8B protein was induced by CPPU during severe drought stress (day 14) at both the tillering and grain-filling stages.…”

Section: Resultsmentioning

confidence: 99%

A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity

Gujjar

Banyen

Chuekong

et al. 2020

Plants

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Drought susceptible rice cultivar PTT1 (Pathumthani1) was treated with drought (−72 kPa) and CPPU (N-2-(chloro-4-pyridyl)-N-phenyl urea) @ 5 mg/L at tillering and grain-filling stages. Plants were tested for the effect of synthetic cytokinin on the parameters influencing the process of photosynthesis. Exogenous spray of CPPU improved the stomatal conductance of rice leaves, which was severely reduced by drought. The abundance intensities of proteins, associated with the stomatal conductance (ZEP, NCED4, PYL9, PYL10, ABI5, SnRK4, Phot1, and Phot2), were also in agreement with the positive impact of CPPU on the stomatal conductance under drought stress. Among the photosynthetic pigments, Chl b contents were significantly reduced by drought stress, whereas CPPU treated plants retained the normal contents of Chl b under drought stress. Subsequently, we examined the abundance intensities of chlorophyll synthase and HCR proteins, implicated in the biosynthesis of chlorophyll pigments and the conversion of Chl b to Chl a, respectively. The results indicated a drought-mediated suppression of chlorophyll synthase. However, CPPU treated plants retained normal levels of chlorophyll synthase under drought stress. In addition, drought stress induced HCR proteins, which might be the cause for reduced Chl b contents in drought stressed plants. Further, CPPU treatment helped the plants sustain photosynthesis at a normal rate under drought stress, which was comparable with well-watered plants. The results were further confirmed by examining the abundance intensities of two key proteins, RAF1 and Rubisco activase, implicated in the assembly and activation of Rubisco, respectively. CPPU treatment reversed the drought mediated suppression of these proteins at both of the growth stages of rice under drought stress. Based on the results, it can be suggested that synthetic cytokinins help the plants sustain photosynthesis at a normal rate under drought stress by positively influencing the determinants of photosynthesis at a molecular level.

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Section: Resultsmentioning

confidence: 99%

A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity

Gujjar

Banyen

Chuekong

et al. 2020

Plants

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“…Unique apocarotenoids are released into the rhizosphere as signals with other organisms. For example, strigolactones are apocarotenoids derived from b-carotene that regulate plant architectural changes (Baz et al, 2018;Gao et al, 2018;Jia et al, 2018). These apocarotenoids inhibit shoot branching above ground and signal below ground with rhizosphere organisms to mediate root architectural changes necessary for symbiosis with beneficial mycorrhizal fungi and mediate resistance to harmful fungi (Al-Babili and Bouwmeester, 2015;Borghi et al, 2016;Decker et al, 2017;Stauder et al, 2018).…”

Section: The Bursting Field Of Apocarotenoidsmentioning

confidence: 99%

Changing Form and Function through Carotenoids and Synthetic Biology

Wurtzel

2018

Plant Physiol.

112

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Carotenoids are structurally diverse pigments and related derivatives that mediate photosynthetic function, responses to biotic and abiotic signals, and control plant architecture. It is these multifaceted roles that make carotenoids uniquely attractive as synthetic biology targets when considering ways to alter plant form and function to meet the needs of food security or new agricultural and industrial applications. This Update seeks to explore how synthetic biology might capitalize on the recent advances made in the carotenoid field. Opportunities are discussed along with the research needed to drive the carotenoid synthetic biology era forward.

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“…This reaction is an important step to form the basic chemical structure of SLs in SL biosynthesis. The role of the CCD8 gene has been characterized in several plant species [ 25 , 36 , 37 , 38 ]. Using the TILLING method, we found two slccd8 mutants (5291 and 2757) of Micro-Tom tomato and characterized their growth traits.…”

Section: Discussionmentioning

confidence: 99%

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Hasegawa

Tsutsumi

Fukushima

et al. 2018

IJMS

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Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.

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CRISPR/Cas9-Mediated Mutagenesis of Carotenoid Cleavage Dioxygenase 8 (CCD8) in Tobacco Affects Shoot and Root Architecture

Cited by 43 publications

References 97 publications

A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity

A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity

Changing Form and Function through Carotenoids and Synthetic Biology

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

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