CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L.

Sun, Qinfu; Li, Lin; Liu, Dongxiao; Wu, Dewei; Fang, Yujie; Wu, Jian; Wang, Youping

doi:10.3390/ijms19092716

Cited by 108 publications

(55 citation statements)

References 82 publications

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“…Sequencing of Brassica genomes has resulted in reference genome sequences for B. napus (Bayer et al ., ; Chalhoub et al ., ; Sun et al ., ), B. rapa (Cai et al ., ; Wang et al ., ), B. oleracea (Liu et al ., ; Parkin et al ., ), B. juncea (Yang et al ., ) and B. nigra (Yang et al ., ), permitting a comprehensive study of R‐genes in these Brassica species. For example, Alamery et al .…”

Section: Introductionmentioning

confidence: 99%

“…Sequencing of Brassica genomes has resulted in reference genome sequences for B. napus Chalhoub et al, 2014;Sun et al, 2018), B. rapa (Cai et al, 2017;Wang et al, 2011), B. oleracea Parkin et al, 2014), B. juncea (Yang et al, 2016) and B. nigra (Yang et al, 2016), permitting a comprehensive study of R-genes in these Brassica species. For example, Alamery et al (2017) found 641, 443 and 249 NBS-LRR genes in B. napus, B. oleracea and B. rapa, respectively, while Yu et al (2014) identified 157, 206 and 167 NBS-LRR genes in B. oleracea, B. rapa and A. thaliana, respectively, which may be due to using different approaches.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation

Dolatabadian

Bayer

Tirnaz

et al. 2019

Plant Biotechnology Journal

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Summary Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content of a species. Therefore, pangenomics analysis is needed to ensure that the genomic diversity within a species is fully represented. Brassica napus is one of the most important oilseed crops in the world and exhibits variability in its resistance genes across different cultivars. Here, we characterized resistance gene distribution across 50 B. napus lines. We identified a total of 1749 resistance gene analogs (RGAs), of which 996 are core and 753 are variable, 368 of which are not present in the reference genome (cv. Darmor‐bzh). In addition, a total of 15 318 SNPs were predicted within 1030 of the RGAs. The results showed that core R‐genes harbour more SNPs than variable genes. More nucleotide binding site‐leucine‐rich repeat (NBS‐LRR) genes were located in clusters than as singletons, with variable genes more likely to be found in clusters. We identified 106 RGA candidates linked to blackleg resistance quantitative trait locus (QTL). This study provides a better understanding of resistance genes to target for genomics‐based improvement and improved disease resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation

Dolatabadian

Bayer

Tirnaz

et al. 2019

Plant Biotechnology Journal

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“…The application of CRISPR-Cas9-targeted mutagenesis in plants has been reported not only in Arabidopsis (Fauser et al, 2014;Feng et al, 2014) but also in crops like wheat (Wang et al, 2014), tomato (Brooks et al, 2014), and rice (Li et al, 2016). In rapeseed, some few studies have already presented targeted genome editing mediated by the CRISPR/Cas9 system (Braatz et al, 2017;Okuzaki et al, 2018;Sun et al, 2018). Therefore, as more and more genes are identified for their function, mutagenesis in rapeseed plant breeding could be managed by more efficient ways than chemical/physical and random ways.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of diverse promising rapeseed (Brassica napus L.) mutants using physical and chemical mutagens

Channaoui

Labhilili²,

Mouhib³

et al. 2019

OCL

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Genetic variability is a prerequisite for any plant breeding program, and mutagenesis is a proven way of creating new variation within a crop germplasm. Novel genetic variability in rapeseed was induced by gamma rays, Ethyl Methane Sulphonate (EMS) and combined mutagen treatment, using various doses and concentrations. The objective was to evaluate and compare the obtained M 2 mutants for important quantitative traits in two contrasted environments. Data on phenological, morphological and agronomic parameters were recorded. A large variability was observed and mutagenic treatments had a significant effect on all traits studied. Compared to control plants, mutant genotypes derived from seeds treated with low EMS concentrations during moderate time were earlier and characterized by a higher number of pods per plant. For high concentration of EMS during long time and for combinations of physical and chemical mutagens, a significant decrease in plant height and stature was noticed, as compared to control. Besides, plants derived from gamma rays-treated seeds exhibited the highest 1000-seed weight. The novel induced variability may be integrated in rapeseed breeding program as a new germplasm with improved agronomic traits. Particularly, EMS1-7-stable mutant may be exploited to develop efficiently and quickly a new rapeseed cultivar with some desirable traits. The present study highlights once more the possibility to bring novel genetic diversity for rapeseed desirable traits improvement through mutation breeding. Keywords: rapeseed / quantitative traits / mutation breeding / EMS treatment / Gamma rays Résumé -Développement et évaluation de divers mutants prometteurs de colza (Brassica napus L.) obtenus par mutagénèse physique et chimique. La variabilité génétique est essentielle pour tout programme d'amélioration de plantes et la mutagénèse s'avère une technique incontestable d'induction de nouvelle variabilité génétique dans les germoplasmes des cultures. Chez le colza, une nouvelle variabilité génétique a été induite après traitement de semences par les rayons gamma, l'EMS et leur combinaison, en utilisant différentes doses et concentrations. L'objectif de cette étude est d'évaluer et comparer les mutants M 2 obtenus pour des caractères quantitatifs, dans deux environnements contrastés. Les paramètres étudiés sont d'ordre phénologique, morphologique et agronomique. Une grande variabilité a été observée et les traitements mutagènes ont eu un effet significatif sur tous les paramètres. Les mutants qui proviennent d'une faible concentration d'EMS, durant une période modérée, sont plus précoces et ont un nombre de siliques plus élevé que la variété témoin (non traitée). Par contre, des mutants dérivés de forte concentration d'EMS, pendant une longue période, et de la combinaison d'EMS avec les rayons gamma, ont montré une réduction significative de la hauteur et de la vigueur des plantes. Par ailleurs, les plantes dérivées de ☆ Contribution to the Topical Issue "Rapeseed / Colza"

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“…In B. napus, a few studies have utilized CRISPR/Cas9 system for editing genes associated with plant/pod development (ALCATRAZ, GA1-3, FRUITFULL, DA1, DA2, CLAVATA, and SPL3), fatty acid synthesis (BnFAD2), and biotic stress response (BnWRKY11and -70) (Sun Q. et al, 2018). The application of CRISPR-Cas9 for climate-resilient transgenic production is yet to be reported in canola ( Table 5).…”

Section: Genome Editing Techniquesmentioning

confidence: 99%

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

et al. 2020

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Impacts of climate change like global warming, drought, flooding, and other extreme events are posing severe challenges to global crop production. Contribution of Brassica napus towards the oilseed industry makes it an essential component of international trade and agroeconomics. Consequences from increasing occurrences of multiple abiotic stresses on this crop are leading to agroeconomic losses making it vital to endow B. napus crop with an ability to survive and maintain yield when faced with simultaneous exposure to multiple abiotic stresses. For an improved understanding of the stress sensing machinery, there is a need for analyzing regulatory pathways of multiple stressresponsive genes and other regulatory elements such as non-coding RNAs. However, our understanding of these pathways and their interactions in B. napus is far from complete. This review outlines the current knowledge of stress-responsive genes and their role in imparting multiple stress tolerance in B. napus. Analysis of network cross-talk through omics data mining is now making it possible to unravel the underlying complexity required for stress sensing and signaling in plants. Novel biotechnological approaches such as transgene-free genome editing and utilization of nanoparticles as gene delivery tools are also discussed. These can contribute to providing solutions for developing climate change resilient B. napus varieties with reduced regulatory limitations. The potential ability of synthetic biology to engineer and modify networks through fine-tuning of stress regulatory elements for plant responses to stress adaption is also highlighted.

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CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L.

Cited by 108 publications

References 82 publications

Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation

Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation

Development and evaluation of diverse promising rapeseed (Brassica napus L.) mutants using physical and chemical mutagens

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

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