CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene

Hirose, Michiko; Hasegawa, Ayumi; Mochida, Keiichi; Matoba, Shogo; Hatanaka, Y.; Inoue, Kimiko; Goto, Tatsuhiko; Kaneda, Hideki; Yamada, Ikuko; Furuse, Tamio; Abe, Kuniya; Uenoyama, Yoshihisa; Tsukamura, Hiroko; Wakana, Shigeharu; Honda, Arata; Ogura, Atsuo

doi:10.1038/srep42476

Cited by 15 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although MITF is at the same locus as the prominent behavioral gene FOXP1, math ability, educational attainment and brain structure traits have been specifically mapped to MITF in human GWASs 21,24,25 . Strikingly, the targeted null-mutation of Asip in a wild-derived mouse strain strongly increased tameness and reduced aggression in comparison to the wild type strain 26 . Domesticated strains of several mammalian species are deficient in Asip and are tamer than wild type (Suppl.…”

Section: Trait Associations Gene Annotation and Brain Relevance Of Qmentioning

confidence: 98%

Genome scans of dog behavior implicate a gene network underlying psychopathology in mammals, including humans

Zapata

Hecht

Serpell

et al. 2020

Preprint

View full text Add to dashboard Cite

Genetic studies show a general factor associated with all human personality and psychopathology, but its basis is unknown. We performed genome scans of 17 normal and problem behaviors in three multi-breed dog cohorts. 21 of 90 mapped loci were supported for the same, or a related, trait in a second cohort. Several of those loci were also associated with brain structure differences across breeds; and five of the respective top-candidate genes are also associated with human brain structure and function. More broadly, the geneset of canine behavioral scans is supported by enrichment for genes mapped for human behavior, personality, psychopathology and brain structure. The biology implicated includes, neurogenesis, axon guidance, angiogenesis, brain structure, alternative splicing, disease association, Hox-family transcription factors, and subiculum expression. Because dog behavior is correlated with body size, we isolated the effect of body size/height in the dog mapping and in the comparative human UK Biobank analyses. Our dog and human findings are consistent with pleiotropy of diverse brain traits with energy metabolism, height, longevity and reproduction. We propose a genetic network underlies neuron birth and development across the life course, and is associated with evolutionary adaptation of behavior and the general psychopathology factor. This understanding has implications for all common psychiatric disorders, and suggests how their risk could be impacted by environmental effects on the IGF1/growth factor signaling-PI3K-AKT-mTOR axis.

show abstract

Section: Trait Associations Gene Annotation and Brain Relevance Of Qmentioning

confidence: 98%

Genome scans of dog behavior implicate a gene network underlying psychopathology in mammals, including humans

Zapata

Hecht

Serpell

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In order to show association of candidate genes and these behaviors, it is useful to establish genetically engineered mice based on these wild strains. However, there are a limited studies that have carried out genetic engineering on the wild strains, due to a lack of information about the appropriate methods for gene-targeting or in vitro manipulation of eggs and fertilized eggs, compared to those used in the laboratory strains 28 – 31 . Previously, Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) genome editing method using microinjection under in vitro conditions was applied to one of the wild strains, MSM, which has originated from Japanese wild mice, to successfully generate a tyrosinase knock-out mice 31 .…”

Section: Introductionmentioning

confidence: 99%

Efficient genome editing in wild strains of mice using the i-GONAD method

Imai

Tanave

Matsuyama

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Wild mouse strains have been used for many research studies, because of the high level of inter-strain genetic and phenotypic variations in them, in addition to the characteristic phenotype maintained from wild mice. However, since application of the current genetic engineering method on wild strains is not easy, there are limited studies that have attempted to apply gene modification techniques in wild strains. Recently, i-GONAD, a new method for genome editing that does not involve any ex vivo manipulation of unfertilized or fertilized eggs has been reported. We applied i-GONAD method for genome editing on a series of wild strains and showed that genome editing is efficiently possible using this method. We successfully made genetically engineered mice in seven out of the nine wild strains. Moreover, we believe that it is still possible to apply milder conditions and improve the efficiencies for the remaining two strains. These results will open avenues for studying the genetic basis of various phenotypes that are characteristic to wild strains. Furthermore, applying i-GONAD will be also useful for other mouse resources in which genetic manipulation is difficult using the method of microinjection into fertilized eggs.

show abstract

“…jp/ mogpl us/# JF1; Takada et al 2013Takada et al , 2015. The genome information is essential and useful for future functional studies based on comprehensive phenotyping and genetic modifications of genetically divergent unique mouse strains created by genome editing (Hirose et al 2017).…”

Section: Adding Value To Collected Mouse Strainsmentioning

confidence: 99%

Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan

et al. 2021

Self Cite

View full text Add to dashboard Cite

The RIKEN BioResource Research Center (BRC) was established in 2001 as a comprehensive biological resource center in Japan. The Experimental Animal Division, one of the BRC infrastructure divisions, has been designated as the core facility for mouse resources within the National BioResource Project (NBRP) by the Japanese government since FY2002. Our activities regarding the collection, preservation, quality control, and distribution of mouse resources have been supported by the research community, including evaluations and guidance on advancing social and research needs, as well as the operations and future direction of the BRC. Expenditure for collection, preservation, and quality-control operations of the BRC, as a national core facility, has been funded by the government, while distribution has been separately funded by users’ reimbursement fees. We have collected over 9000 strains created mainly by Japanese scientists including Nobel laureates and researchers in cutting-edge fields and distributed mice to 7000 scientists with 1500 organizations in Japan and globally. Our users have published 1000 outstanding papers and a few dozen patents. The collected mouse resources are accessible via the RIKEN BRC website, with a revised version of the searchable online catalog. In addition, to enhance the visibility of useful strains, we have launched web corners designated as the “Mouse of the Month” and “Today’s Tool and Model.” Only high-demand strains are maintained in live colonies, while other strains are cryopreserved as embryos or sperm to achieve cost-effective management. Since 2007, the RIKEN BRC has built up a back-up facility in the RIKEN Harima branch to protect the deposited strains from disasters. Our mice have been distributed with high quality through the application of strict microbial and genetic quality control programs that cover a globally accepted pathogens list and mutated alleles generated by various methods. Added value features, such as information about users’ publications, standardized phenotyping data, and genome sequences of the collected strains, are important to facilitate the use of our resources. We have added and disseminated such information in collaboration with the NBRP Information Center and the NBRP Genome Information Upgrading Program. The RIKEN BRC has participated in international mouse resource networks such as the International Mouse Strain Resource, International Mouse Phenotyping Consortium, and Asian Mouse Mutagenesis and Resource Association to facilitate the worldwide use of high-quality mouse resources, and as a consequence it contributes to reproducible life science studies and innovation around the globe.

show abstract

CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene

Cited by 15 publications

References 38 publications

Genome scans of dog behavior implicate a gene network underlying psychopathology in mammals, including humans

Genome scans of dog behavior implicate a gene network underlying psychopathology in mammals, including humans

Efficient genome editing in wild strains of mice using the i-GONAD method

Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan

Contact Info

Product

Resources

About