CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement

Zhang, Shujuan; Zhang, Rongzhi; Gao, Jie; Song, Guang; Li, Jihu; Li, Wei; Qi, Yiping; Li, Yulian; Li, Genying

doi:10.1111/pbi.13647

Cited by 46 publications

(26 citation statements)

References 11 publications

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“…Several loss-of-function mutations in the PSY1 gene of tomato (genetic locus r ) have been isolated ( r 2997 , r 3756 , r y , yft2 , PI114490; Fray and Grierson, 1993 ; Yuan et al, 2008 ; Gady et al, 2012 ; Kachanovsky et al, 2012 ; Chen et al, 2019 ). Likewise, CRISPR mutants have also been generated for PSY1 in tomato and maize, PSY in wheat, and both PSY1 and PSY2 in carrots ( Zhu et al, 2016 ; Dahan-Meir et al, 2018 ; D’Ambrosio et al, 2018 ; Oleszkiewicz et al, 2021 ; Zhang et al, 2021 ). Reduced accumulation of carotenoids characterize CRISPR mutants in wheat, while the albino plants and white seeds are observed in maize ( Zhu et al, 2016 ; Zhang et al, 2021 ).…”

Section: Carotenoid Biosynthesis – the Central Pathwaymentioning

confidence: 99%

“…Likewise, CRISPR mutants have also been generated for PSY1 in tomato and maize, PSY in wheat, and both PSY1 and PSY2 in carrots ( Zhu et al, 2016 ; Dahan-Meir et al, 2018 ; D’Ambrosio et al, 2018 ; Oleszkiewicz et al, 2021 ; Zhang et al, 2021 ). Reduced accumulation of carotenoids characterize CRISPR mutants in wheat, while the albino plants and white seeds are observed in maize ( Zhu et al, 2016 ; Zhang et al, 2021 ). In carrots, PSY1 and PSY2 mutants display pale orange to yellow pigmentation in callus, with PSY2 is critical for carotenogenesis in roots ( Oleszkiewicz et al, 2021 ).…”

Section: Carotenoid Biosynthesis – the Central Pathwaymentioning

confidence: 99%

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The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

Gupta

Hirschberg

2022

Front. Plant Sci.

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Carotenoids comprise the most widely distributed natural pigments. In plants, they play indispensable roles in photosynthesis, furnish colors to flowers and fruit and serve as precursor molecules for the synthesis of apocarotenoids, including aroma and scent, phytohormones and other signaling molecules. Dietary carotenoids are vital to human health as a source of provitamin A and antioxidants. Hence, the enormous interest in carotenoids of crop plants. Over the past three decades, the carotenoid biosynthesis pathway has been mainly deciphered due to the characterization of natural and induced mutations that impair this process. Over the year, numerous mutations have been studied in dozens of plant species. Their phenotypes have significantly expanded our understanding of the biochemical and molecular processes underlying carotenoid accumulation in crops. Several of them were employed in the breeding of crops with higher nutritional value. This compendium of all known random and targeted mutants available in the carotenoid metabolic pathway in plants provides a valuable resource for future research on carotenoid biosynthesis in plant species.

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Section: Carotenoid Biosynthesis – the Central Pathwaymentioning

confidence: 99%

Section: Carotenoid Biosynthesis – the Central Pathwaymentioning

confidence: 99%

The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

Gupta

Hirschberg

2022

Front. Plant Sci.

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“…CRISPR/Cas9-mediated genome editing technology creates the opportunity to establish powerful germplasm resources ( Zhang et al, 2021 ). However, there are few reports on germplasm resources of optimal plant-type, and no systematic study of LRR-RLK family in B. napus .…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus

et al. 2022

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The leucine-rich repeat receptor-like protein kinase (LRR-RLK) family represents the largest group of RLKs in plants and plays vital roles in plant growth, development and the responses to environmental stress. Although LRR-RLK families have been identified in many species, they have not yet been reported in B. napus. In this study, a total of 444 BnLRR-RLK genes were identified in the genome of Brassica napus cultivar “Zhongshuang 11” (ZS11), and classified into 22 subfamilies based on phylogenetic relationships and genome-wide analyses. Conserved motifs and gene structures were shared within but not between subfamilies. The 444 BnLRR-RLK genes were asymmetrically distributed on 19 chromosomes and exhibited specific expression profiles in different tissues and in response to stress. We identified six BnBRI1 homologs and obtained partial knockouts via CRISPR/Cas9 technology, generating semi-dwarf lines without decreased yield compared with controls. This study provides comprehensive insight of the LRR-RLK family in B. napus. Additionally, the semi-dwarf lines expand the “ideotype” germplasm resources and accelerate the breeding process for B. napus.

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“…Knocking out the ZmCEP1 gene through CRISPR/Cas9 significantly increased the ear height, grain size and yield of corn plants [15]. CRISPR/Cas9-mediated genome editing improved wheat grain quality, successfully transformed soft wheat into durum wheat, and increased the genetic resources for wheat (Triticum aestivum) grain hardness improvement [16]. CRISPR/Cas9 technology is used in soybean (Glycine max) to specifically design double sgRNA/Cas9 to induce targeted deletion of the soybean GmFATB1 gene.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

Zhang¹,

Li²,

Hu³

et al. 2022

Phyton

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Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T 2 generation positive transgenic soybean plants identified by PCR and Southern hybridization. The results showed that the overexpression vector plant GmHsps_p23-like gene expression increased. After rehydration, the transgenic overexpression plants returned to normal growth, and the damage to the plants was low. After drought stress, the SOD and POD activities and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group, and had a stronger drought resistance. It showed that the expression of soybean GmHsps_p23-like gene can improve the drought resistance of soybean. The cloning and functional verification of soybean GmHsps_p23-like gene had not been reported yet. This is the first time that PCR technology has been used to amplify the soybean GmHsps_p23-like gene and construct an expression vector for this gene. This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties.

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CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement

Cited by 46 publications

References 11 publications

The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus

Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

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