CRISPR/Cas9-mediated deletion of a kinetoplast-associated gene attenuates virulence inLeishmania majorparasites
Fatemeh Darzi,
Ali Khamesipour,
Maryam Bahrami
et al.
Abstract:The CRISPR/Cas9 system has emerged as a powerful tool for precise genome editing, allowing for the deletion of genes, generation of point mutations, and addition of tags to endogenous genes. We employed an efficient CRISPR/Cas9 technique in Leishmania major to assess its efficiency in editing a kDNA-associated gene, universal minicircle sequence binding protein (UMSBP), which is involved in mitochondrial respiration and kinetoplast division. We generated UMSBP C-tagged and UMSBP single knockout L. major (LmUMS… Show more
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