CRISPR/Cas9-Induced Mutagenesis of TMS5 Confers Thermosensitive Genic Male Sterility by Influencing Protein Expression in Rice (Oryza sativa L.)

Abstract

The development of thermosensitive genic male sterile (TGMS) lines is the key to breeding two-line hybrid rice, which has been widely applied in China to increase grain yield. CRISPR/Cas9 has been widely used in genome editing to create novel mutants in rice. In the present study, a super grain quality line, GXU 47, was used to generate a new TGMS line with specific mutations in a major TGMS gene tms5 generated with CRISPR/Cas9-mediated genome editing in order to improve the rice quality of two-line hybrids. A mutagenesis efficiency level of 75% was achieved, and three homozygous T-DNA-free mutant lines were screened out. The mutants exhibited excellent thermosensitive male fertility transformation characteristics with complete male sterility at ≥24 °C and desirable male fertility at around 21 °C. Proteomic analysis based on isobaric tags for relative and absolute quantification (iTRAQ) was performed to unveil the subsequent proteomic changes. A total of 192 differentially expressed proteins (DEPs), including 35 upregulated and 157 downregulated, were found. Gene ontology (GO) analysis revealed that the DEPs were involved in a single-organism biosynthetic process, a single-organism metabolic process, oxidoreductase activity, and catalytic activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEPs were involved in ubiquinone and other terpenoid quinone biosynthesis, the biosynthesis of secondary metabolites, metabolic pathways, and phenylpropanoid biosynthesis. Our study shows that high mutation efficiency was achieved in both target sites, and T-DNA-free mutant lines were obtained in the T1 generation. The present study results prove that it is feasible and efficient to generate an excellent mutant line with CRISPR/Cas9, which provides a novel molecular mechanism of male sterility caused by the mutation of tms5.

Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications

“…(Huang et al, 2014). Recent studies revealed the molecular mechanism of tms5 leading to male sterility in rice to easily obtain excellent TGMS lines (Fang et al, 2022) and potentially applicable in other crops. CRISPR/Cas9-engineered mutation of TMS5 also resulted in the formation of thermosensitive male sterility in maize (Li et al, 2017).…”

CRISPR/Cas Technology Revolutionizes Crop Breeding

“…Many male-sterile-related genes have been well characterized in maize [100], rice [101][102][103][104][105], wheat [106], and soybean [107], and improved the understanding of the molecular mechanisms that control male sterility in crops. Importantly, increasing CRISPR-edited malesterile lines have been generated in maize by knocking out ZmMS26 or ms8 [108,109], rice by editing TMS5, OsOPR7, or CYP703A3 [99,[110][111][112][113][114][115], wheat by targeting TaNP1, Ms1, or Ms45 [116][117][118], foxtail millet [119], and soybean [120]. Thus, the CRISPR/Cas technology has provided a powerful tool for the generation of male-sterile lines and will greatly promote commercial hybrid seed production in different crops.…”