CRISPR/Cas9 Gene Editing: An Unexplored Frontier for Forest Pathology

Dort, Erika; Tanguay, Philippe; Hamelin, Richard

doi:10.3389/fpls.2020.01126

Cited by 35 publications

(20 citation statements)

References 137 publications

(215 reference statements)

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“…Marker-Assisted Selection-MAS (Butcher and Southerton, 2007;Muranty et al, 2014) and Backcrossing-MAB (Herzog and Frisch, 2011) provide frameworks to pyramid target genetic variants of simple Mendelian traits, which are those regulated by few major genes (e.g., resistance to biotic stresses). Gene editing (Doudna and Charpentier, 2014;Dort et al, 2020) and transgenics (Campbell et al, 2003) can also transfer or silence allelic variants of major effects within a single generation (Pereira-Lorenzo et al, 2019). These may replicate the success of tolerant chestnuts (Alcaide et al, 2019a;Westbrook et al, 2019) and promote reproductive sterility (Meilan et al, 2001;Fritsche et al, 2018).…”

Section: Predictive Breeding Promises Boosting Forest Tree Genetic Immentioning

confidence: 99%

Modern Strategies to Assess and Breed Forest Tree Adaptation to Changing Climate

2020

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Studying the genetics of adaptation to new environments in ecologically and industrially important tree species is currently a major research line in the fields of plant science and genetic improvement for tolerance to abiotic stress. Specifically, exploring the genomic basis of local adaptation is imperative for assessing the conditions under which trees will successfully adapt in situ to global climate change. However, this knowledge has scarcely been used in conservation and forest tree improvement because woody perennials face major research limitations such as their outcrossing reproductive systems, long juvenile phase, and huge genome sizes. Therefore, in this review we discuss predictive genomic approaches that promise increasing adaptive selection accuracy and shortening generation intervals. They may also assist the detection of novel allelic variants from tree germplasm, and disclose the genomic potential of adaptation to different environments. For instance, natural populations of tree species invite using tools from the population genomics field to study the signatures of local adaptation. Conventional genetic markers and whole genome sequencing both help identifying genes and markers that diverge between local populations more than expected under neutrality, and that exhibit unique signatures of diversity indicative of "selective sweeps." Ultimately, these efforts inform the conservation and breeding status capable of pivoting forest health, ecosystem services, and sustainable production. Key long-term perspectives include understanding how trees' phylogeographic history may affect the adaptive relevant genetic variation available for adaptation to environmental change. Encouraging "big data" approaches (machine learning-ML) capable of comprehensively merging heterogeneous genomic and ecological datasets is becoming imperative, too.

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Section: Predictive Breeding Promises Boosting Forest Tree Genetic Immentioning

confidence: 99%

Modern Strategies to Assess and Breed Forest Tree Adaptation to Changing Climate

2020

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“…Therefore, genetic modification (GM) provides the potential for transformation in shorter timeframes but is challenged by existing genetically modified organism (GMO) laws. Genome editing (GE), which may generate mutations in sites, allows for the fast implementation of specific changes and is less restricted worldwide than genetically modified technology [ 240 , 241 ]. Genome engineering in forestry is urgently needed given the rise in human activity and the effects of climate change (for instance, changes in rainfall distribution and the increase in severe droughts).…”

Section: Genome-wide Association Studies (Gwas)mentioning

confidence: 99%

“…These studies suggest that the sequence context at or near the target sites may affect mutagenesis results. Available findings indicate persistent CRISPR-induced mutations and related phenotypes across many clonal generations enabling commercial production of elite trees propagated vegetatively [ 240 , 258 , 266 ].…”

Section: Genome-wide Association Studies (Gwas)mentioning

confidence: 99%

“…They are a worldwide concern for forest ecosystems and must be handled as soon as possible. Ideally, CRISPR/Cas9 should be integrated into forest development projects to create more effective disease resistance methods for long-term forest sustainability [ 240 ]. For example, using CRISPR/Cas9 to combat Dutch elm disease (DED) pathogen Ophiostoma novo-ulmi is another intriguing potential, in which it has been demonstrated that these genes are excellent candidates for CRISPR/Cas9 gene editing to generate knockout mutants with decreased capacity to switch the DED pathogen throughout the elm tree life cycle [ 270 , 271 ].…”

Section: Genome-wide Association Studies (Gwas)mentioning

confidence: 99%

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Achievements and Challenges of Genomics-Assisted Breeding in Forest Trees: From Marker-Assisted Selection to Genome Editing

Ahmar

Ballesta²,

Ali

et al. 2021

IJMS

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Forest tree breeding efforts have focused mainly on improving traits of economic importance, selecting trees suited to new environments or generating trees that are more resilient to biotic and abiotic stressors. This review describes various methods of forest tree selection assisted by genomics and the main technological challenges and achievements in research at the genomic level. Due to the long rotation time of a forest plantation and the resulting long generation times necessary to complete a breeding cycle, the use of advanced techniques with traditional breeding have been necessary, allowing the use of more precise methods for determining the genetic architecture of traits of interest, such as genome-wide association studies (GWASs) and genomic selection (GS). In this sense, main factors that determine the accuracy of genomic prediction models are also addressed. In turn, the introduction of genome editing opens the door to new possibilities in forest trees and especially clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9). It is a highly efficient and effective genome editing technique that has been used to effectively implement targetable changes at specific places in the genome of a forest tree. In this sense, forest trees still lack a transformation method and an inefficient number of genotypes for CRISPR/Cas9. This challenge could be addressed with the use of the newly developing technique GRF-GIF with speed breeding.

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“…In October 2020, the Nobel Committee announced the award of Nobel Prize in Chemistry to Dr. Emmanuelle Charpentier and Dr. Jennifer Doudna for their pioneering work in precise genome editing with the clustered regularly interspaced short palindromic repeats (CRISPR) technology. The basic features of CRISPR were first recognized by Japanese scientists in 1987 and officially termed as “CRISPR” in 2002, but it was not further developed until the early 2000s, when it was used as a powerful genome editing platform [ 1 , 2 ]. This technology allows exceptionally precise genome editing in a wide range of species and extends our ability to investigate the contribution of genetic factors to various unexplained phenotypes and diseases.…”

mentioning

confidence: 99%

CRISPR/Cas9 from bench to bedside: what clinicians need to know before application?

2020

Military Med Res

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In October 2020, Dr. Emmanuelle Charpentier and Dr. Jennifer Doudna won the Nobel Prize in Chemistry for their pioneering work in precise genome editing using the CRISPR technology. Although CRISPR technology has developed rapidly in the last decade, there are still many uncertainties before eventual use in clinical settings. In this mini review, we summarize the current efforts in addressing the limitations of CRISPR technology and future directions.

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CRISPR/Cas9 Gene Editing: An Unexplored Frontier for Forest Pathology

Cited by 35 publications

References 137 publications

Modern Strategies to Assess and Breed Forest Tree Adaptation to Changing Climate

Modern Strategies to Assess and Breed Forest Tree Adaptation to Changing Climate

Achievements and Challenges of Genomics-Assisted Breeding in Forest Trees: From Marker-Assisted Selection to Genome Editing

CRISPR/Cas9 from bench to bedside: what clinicians need to know before application?

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