CRISPR/Cas9 edited <i>HSFA6a and HSFA6b</i> of <i>Arabidopsis thaliana</i> offers ABA and osmotic stress insensitivity by modulation of ROS homeostasis

Wang, Wenjing; Chen, Qingbin; Singh, Prashant Kumar; Huang, Yuanyuan; Pei, Dongli

doi:10.1080/15592324.2020.1816321

Cited by 31 publications

(22 citation statements)

References 41 publications

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“…We found that SsKAI2 OEs promoted the expression level of ABA responsive genes, such as ABI3, ABI5, MYB96, MYB3R2, and ABF1 under cold stress (Figure 8), which is an agreement with the results of Li et al (2017) suggesting that KAI2 might regulate the abiotic stress tolerance could be ABA-dependent. The relationship of ABA with redox homeostasis is well documented in different studies Postiglione and Muday, 2020;Wenjing et al, 2020). Altogether, our results are suggesting that SsKAI2 enhanced response to ABA and induced expression level of ABA-responsive genes might be a pathway leading to redox homeostasis under cold stress.…”

Section: Karrikins Insensitive2 (Kai2supporting

confidence: 77%

Overexpression of Karrikins Receptor Gene Sapium sebiferum KAI2 Promotes the Cold Stress Tolerance via Regulating the Redox Homeostasis in Arabidopsis thaliana

Shah

Yao

et al. 2021

Front. Plant Sci.

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KARRIKINS INSENSITIVE2 (KAI2) is the receptor gene for karrikins, recently found to be involved in seed germination, hypocotyl development, and the alleviation of salinity and osmotic stresses. Nevertheless, whether KAI2 could regulate cold tolerance remains elusive. In the present study, we identified that Arabidopsis mutants of KAI2 had a high mortality rate, while overexpression of, a bioenergy plant, Sapium sebiferum KAI2 (SsKAI2) significantly recovered the plants after cold stress. The results showed that the SsKAI2 overexpression lines (OEs) had significantly increased levels of proline, total soluble sugars, and total soluble protein. Meanwhile, SsKAI2 OEs had a much higher expression of cold-stress-acclimation-relate genes, such as Cold Shock Proteins and C-REPEAT BINDING FACTORS under cold stress. Moreover, the results showed that SsKAI2 OEs were hypersensitive to abscisic acid (ABA), and ABA signaling genes were w significantly affected in SsKAI2 OEs under cold stress, suggesting a potential interaction between SsKAI2 and ABA downstream signaling. In SsKAI2 OEs, the electrolyte leakage, hydrogen peroxide, and malondialdehyde contents were reduced under cold stress in Arabidopsis. SsKAI2 OEs enhanced the anti-oxidants like ascorbate peroxidase, catalase, peroxidase, superoxide dismutase, and total glutathione level under cold stress. Conclusively, these results provide novel insights into the understanding of karrikins role in the regulation of cold stress adaptation.

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Section: Karrikins Insensitive2 (Kai2supporting

confidence: 77%

Overexpression of Karrikins Receptor Gene Sapium sebiferum KAI2 Promotes the Cold Stress Tolerance via Regulating the Redox Homeostasis in Arabidopsis thaliana

Shah

Yao

et al. 2021

Front. Plant Sci.

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“…The genome editing method is a powerful technique for producing varieties; thus, several genome-edited plants are currently being produced [87,88]. Mutations in HSFA6a and HSFA6b, created by CRISPR/Cas9, are more tolerant to abiotic stresses such as ABA, mannitol, and sodium chloride [89]. Researchers have developed several mutants, such as the tomato ead1 mutant and soybean hsp90A2 mutant, using CRISPR/Cas9.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Abdellatif

Yuan

Renhu

et al. 2022

IJMS

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Heat stress (HS) is a prevalent negative factor affecting plant growth and development, as it is predominant worldwide and threatens agriculture on a large scale. PHYTOCHROMES (PHYs) are photoreceptors that control plant growth and development, and the stress signaling response partially interferes with their activity. PHYA, B1, and B2 are the most well-known PHY types in tomatoes. Our study aimed to identify the role of tomato ‘Money Maker’ phyA and phyB1B2 mutants in stable and fluctuating high temperatures at different growth stages. In the seed germination and vegetative growth stages, the phy mutants were HS tolerant, while during the flowering stage the phy mutants revealed two opposing roles depending on the HS exposure period. The response of the phy mutants to HS during the fruiting stage showed similarity to WT. The most obvious stage that demonstrated phy mutants’ tolerance was the vegetative growth stage, in which a high degree of membrane stability and enhanced water preservation were achieved by the regulation of stomatal closure. In addition, both mutants upregulated the expression of heat-responsive genes related to heat tolerance. In addition to lower malondialdehyde accumulation, the phyA mutant enhanced proline levels. These results clarified the response of tomato phyA and phyB1B2 mutants to HS.

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“…The orthologs of HSFA6b and HSFA6a are expressed in the primary and lateral roots of Arabidopsis (Hwang et al, 2014). HSFA6b also regulates root elongation in an ABA‐dependent manner (Wenjing et al, 2020) (Figure 3). ABA treatment or signalling is required for HSFA6a and HSFA6b expression and translocation to the nucleus.…”

Section: Phytohormones Regulation and Their Interactions In Roots Exp...mentioning

confidence: 99%

“…Wenjing et al, 2020) (Figure3). ABA treatment or signalling is required for HSFA6a and HSFA6b expression and translocation to the nucleus.…”

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confidence: 99%

Genetic and molecular mechanisms underlying root architecture and function under heat stress—A hidden story

Tiwari

Kumar

Min

et al. 2022

Plant Cell & Environment

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Heat stress events are resulting in a significant negative impact on global food production. The dynamics of cellular, molecular and physiological homoeostasis in aboveground parts under heat stress are extensively deciphered. However, root responses to higher soil/air temperature or stress signalling from shoot to root are limited. Therefore, this review presents a holistic view of root physio-morphological and molecular responses to adapt under hotter environments. Heat stress reprogrammes root cellular machinery, including crosstalk between genes, phytohormones, reactive oxygen species (ROS) and antioxidants. Spatio-temporal regulation and long-distance transport of phytohormones, such as auxin, cytokinin and abscisic acid (ABA) determine the root growth and development under heat stress. ABA cardinally integrates a signalling pathway involving heat shock factors, heat shock proteins and ROS to govern heat stress responses. Additionally, epigenetic modifications by transposable elements, DNA methylation and acetylation also regulate root growth under heat stress. Exogenous application of chemical compounds or biological agents such as ascorbic acid, metal ion chelators, fungi and bacteria can alleviate heat stress-induced reduction in root biomass. Future research should focus on the systemic effect of heat stress from shoot to root with more detailed investigations to decipher the molecular cues underlying the roots architecture and function.

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CRISPR/Cas9 edited HSFA6a and HSFA6b of Arabidopsis thaliana offers ABA and osmotic stress insensitivity by modulation of ROS homeostasis

Cited by 31 publications

References 41 publications

Overexpression of Karrikins Receptor Gene Sapium sebiferum KAI2 Promotes the Cold Stress Tolerance via Regulating the Redox Homeostasis in Arabidopsis thaliana

Overexpression of Karrikins Receptor Gene Sapium sebiferum KAI2 Promotes the Cold Stress Tolerance via Regulating the Redox Homeostasis in Arabidopsis thaliana

Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Genetic and molecular mechanisms underlying root architecture and function under heat stress—A hidden story

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