CRISPR/Cas9-based genome editing of banana for disease resistance

Tripathi, Leena; Ntui, Valentine Otang; Tripathi, Jaindra Nath

doi:10.1016/j.pbi.2020.05.003

Cited by 81 publications

(64 citation statements)

References 35 publications

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“…Recent advances in genome editing technologies using site-directed nucleases (SDNs), such as meganucleases, encoded by mobile genetic elements or introns, zinc-finger nucleases derived from eukaryotic Cys 2 His 2 zinc finger proteins covalently linked to the nuclease domain of the type IIS restriction enzyme Fok1, transcription activator-like effector nucleases (TALENs) from TALEs of bacteria Xanthomonas linked with the Fok1 nuclease domain, and CRISPR/ Cas from the adaptive immunity system of Streptococcus pyogenes have enabled plant scientists to manipulate desired genes in crop plants (Tripathi et al, 2020). Among these nucleases, CRISPR/Cas9 is the most powerful and versatile tool for crop genome editing because of its simplicity, design flexibility, and high efficiency and its ability to simultaneously edit multiple genes (Ntui et al, 2020;Tripathi et al, 2019) Based on the type of repair, the editing can be performed by SDN1, SDN2, or SDN3 (Modrzejewski et al, 2019).…”

Section: Crispr/cas9 Gene Editing Strategy To Develop Anthracnose Resistancementioning

confidence: 99%

Strategies to combat the problem of yam anthracnose disease: Status and prospects

Ntui

Uyoh

Ita

et al. 2021

Molecular Plant Pathology

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Section: Crispr/cas9 Gene Editing Strategy To Develop Anthracnose Resistancementioning

confidence: 99%

Strategies to combat the problem of yam anthracnose disease: Status and prospects

Ntui

Uyoh

Ita

et al. 2021

Molecular Plant Pathology

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“…is an important staple food crop and a source of income for resource‐poor farmers in more than 136 tropical and sub‐tropical countries with an annual production of 155 million tons (FAOSTAT, 2018). Many diseases severely constrain banana production, particularly where many pathogens co‐exist (Tripathi et al ., 2020). Banana Xanthomonas wilt (BXW) caused by Xanthomonas campestris pv.…”

Section: Figurementioning

confidence: 99%

CRISPR/Cas9‐mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease

Tripathi

Ntui

Shah

et al. 2021

Plant Biotechnology Journal

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“…fijiensis pathosystem, it offers considerable potential for the development of banana varieties with multiple and durable resistance and tolerance (Tripathi et al, 2019(Tripathi et al, , 2020.…”

Section: Limitations Of the Review And Future Researchmentioning

confidence: 99%

Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review

et al. 2021

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Bananas are an important staple food crop in tropical and subtropical regions in Asia, sub-Saharan Africa, and Central and South America. The plant is affected by numerous diseases, with the fungal leaf disease black Sigatoka, caused by Mycosphaerella fijiensis Morelet [anamorph: Pseudocercospora fijiensis (Morelet) Deighton], considered one of the most economically important phytosanitary problem. Although the development of resistant cultivars is recognized as most effective method for long term control of the disease, the majority of today's cultivars are susceptible. In order to gain insights into this pathosystem, this first systematic literature review on the topic is presented. Utilizing six databases (PubMed Central, Web of Science, Google Academic, Springer, CAPES and Scopus Journals) searches were performed using pre-established inclusion and exclusion criteria. From a total of 3,070 published studies examined, 24 were relevant with regard to the Musa-P. fijiensis pathosystem. Relevant papers highlighted that resistant and susceptible cultivars clearly respond differently to infection by this pathogen. M. acuminata wild diploids such as Calcutta 4 and other diploid cultivars can harbor sources of resistance genes, serving as parentals for the generation of improved diploids and subsequent gene introgression in new cultivars. From the sequenced reference genome of Musa acuminata, although the function of many genes in the genome still require validation, on the basis of transcriptome, proteome and biochemical data, numerous candidate genes and molecules have been identified for further evaluation through genetic transformation and gene editing approaches. Genes identified in the resistance response have included those associated with jasmonic acid and ethylene signaling, transcription factors, phenylpropanoid pathways, antioxidants and pathogenesis-related proteins. Papers in this study also revealed gene-derived markers in Musa applicable for downstream application in marker assisted selection. The information gathered in this review furthers understanding of the immune response in Musa to the pathogen P. fijiensis and is relevant for genetic improvement programs for bananas and plantains for control of black Sigatoka.

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CRISPR/Cas9-based genome editing of banana for disease resistance

Cited by 81 publications

References 35 publications

Strategies to combat the problem of yam anthracnose disease: Status and prospects

Strategies to combat the problem of yam anthracnose disease: Status and prospects

CRISPR/Cas9‐mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease

Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review

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