2019
DOI: 10.1002/smtd.201800473
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CRISPR/Cas9‐Based Genome Editing and its Applications for Functional Genomic Analyses in Plants

Abstract: The ability to modify complex genomes precisely to create specific mutants is the holy grail of basic research and applied genetics in the postgenomic era. Programmable sequence‐specific nucleases (e.g., zinc finger nucleases, transcription activator‐like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) systems (e.g., CRISPR/Cas9)), which induce targeted DNA breaks that are then repaired by endogenous repair machinery to generate mutants in a va… Show more

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Cited by 28 publications
(13 citation statements)
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References 205 publications
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“…Several genome editing technologies with the ability to change the code of life with high specificity have been developed recently (Li et al 2019 ). Advances in genome editing have revolutionized life science, including plant science.…”
Section: Introductionmentioning
confidence: 99%
“…Several genome editing technologies with the ability to change the code of life with high specificity have been developed recently (Li et al 2019 ). Advances in genome editing have revolutionized life science, including plant science.…”
Section: Introductionmentioning
confidence: 99%
“…Two major components are essential for typical engineered CRISPR-Cas systems, a Cas endonuclease protein, and a single guide RNA (sgRNA) of 20 nucleotide sequences that guide the Cas enzyme to the target sequence for introducing the double-stranded break (DSB) ( Mahfouz et al, 2014 ). Multiple variants of Cas9 and gRNA are available according to their novel application in the field of genetic engineering in plants ( Chen et al, 2019 ; Li et al, 2019 ).…”
Section: Crispr-based Genome Editing Tool: Components and Mechanismmentioning
confidence: 99%
“…There has been a remodeling of the Cas protein, specifically the protein designated SpCas9 or simply Cas9, which is from Streptococcus pyogenes. Generation of these protein variants increased options beyond the ability to induce double strand breaks to now having a variant that cleaves only one strand, referred to as a Cas9 nickase, and a variant that binds DNA, but does not cleave, referred to as a "dead" Cas9 (dCas9) (19). These variants have been exploited for development of new designs that make it possible to achieve modifications that range from single base pair changes to exchanging of alleles [20,21].…”
Section: Expanding the Crispr/cas Toolboxmentioning
confidence: 99%
“…The initially available and subsequently most widely used Cas protein, Cas9, results in blunt-end cuts of the DNA a few base pairs upstream from the recognized PAM sequence, 5'-NGG-3' (N = A, T, G, C) (19). Cas9 has been shown to be effective in editing genomes of numerous organisms including plants with some groups developing plant codon optimized versions depending on the species of interest.…”
Section: The Cas Protein Collectionmentioning
confidence: 99%
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