CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging

Abstract

The biological state at subcellular level is highly relevant to many diseases, and the monitoring of organelles such as mitochondria is crucial based on this. However, most DNA and protein...

Exogenous and Endogenous Dual-Activated Nanoladder for Precise Imaging of Mitochondrial Ferroptosis-Related Inhibition miRNA with Tumor Cell Specificity

“…As shown in Figure S10, the fluorescence signal of UC-EH2-TPP and Mito-tracker matched well with each other with a Pearson’s correlation coefficient of 0.96, while the fluorescence signal of UC-EH2 (without TPP) and Mito-tracker matched poorly with each other with a Pearson’s correlation coefficient of 0.28, confirming the key role of TPP for mitochondria targeting. It is reported that TPP has good lipophilicity and positivity, allowing nanoparticles to target mitochondria and also allowing them to escape from lysosomes. , Lysosome colocalization experiments revealed that the green channel of LysoTracker matched poorly with the red channel of the probe after 4 h incubation with a Pearson’s correlation coefficient of ∼0.26, confirming that the probe successfully escaped from lysosomes (Figure C). Furthermore, the reaction time was optimized to be 4h (Figure S11).…”

“…It is reported that TPP has good lipophilicity and positivity, allowing nanoparticles to target mitochondria and also allowing them to escape from lysosomes. 23,24 Lysosome colocalization experiments revealed that the green channel of LysoTracker matched poorly with the red channel of the probe after 4 h incubation with a Pearson's correlation coefficient of ∼0.26, confirming that the probe successfully escaped from lysosomes (Figure 4C). Furthermore, the reaction time was optimized to be 4h (Figure S11).…”

Single-Molecule Evaluation of the SARS-CoV-2 Nucleocapsid Protein Using Gold Particle-in-a-Frame Nanostructures Enhanced Fluorescent Assay

“…Given the excellent sensitivity and selectivity of this strategy, we further explored the potential of this method using the TIRF-based single-molecule counting method. The fluorescent dots could be counted at the single-molecule counting mode with extremely high sensitivity. − A new platform for detecting low-abundance N proteins is made available, which is vital for the early diagnosis and treatment of COVID-19.…”

Light and endogenous enzyme triggered plasmonic antennas for accurate subcellular molecular imaging with enhanced spatial resolution

“…4 and S12, † aer 4 h incubation, the Cy3 channel of the LP and the Cy5.5 channel of the EP anastomosed well with the green channel of Mito-Tracker Green, with a Pearson's correlation coefficient of ∼0.85 and ∼0.87, respectively, indicating the prominent localization of both the LP and EP in mitochondria. According to literature reports, 27,28 TPP has good lipophilic and positive properties, which can not only help nanoparticles target mitochondria, but also help them escape from lysosomes. Accordingly, in order to verify lysosome escape, lysosome colocalization experiments were also performed.…”