CRISPR/Cas9 and Cas13a systems: a promising tool for plant breeding and plant defence

Badar, Uzma; Venkataraman, Srividhya; Hefferon, Kathleen

doi:10.1016/b978-0-12-821910-2.00002-3

Cited by 1 publication

(1 citation statement)

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“…Gene knockout by CRISPR/Cas technology has another advantage compared to many other gene-silencing technologies, namely, the T-DNA carrying the CRISPR/Cas can be removed from the genome through segregation, thus significantly reducing the burdens in the governmental regulatory process. In summary, the maturation of the enabling technologies described here has opened the door to a new era of precision molecular breeding of sweet potato [142,143].…”

Section: Discussionmentioning

confidence: 95%

Engineering Properties of Sweet Potato Starch for Industrial Applications by Biotechnological Techniques including Genome Editing

Lyu

Ahmed

Fan

et al. 2021

IJMS

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Sweet potato (Ipomoea batatas) is one of the largest food crops in the world. Due to its abundance of starch, sweet potato is a valuable ingredient in food derivatives, dietary supplements, and industrial raw materials. In addition, due to its ability to adapt to a wide range of harsh climate and soil conditions, sweet potato is a crop that copes well with the environmental stresses caused by climate change. However, due to the complexity of the sweet potato genome and the long breeding cycle, our ability to modify sweet potato starch is limited. In this review, we cover the recent development in sweet potato breeding, understanding of starch properties, and the progress in sweet potato genomics. We describe the applicational values of sweet potato starch in food, industrial products, and biofuel, in addition to the effects of starch properties in different industrial applications. We also explore the possibility of manipulating starch properties through biotechnological means, such as the CRISPR/Cas-based genome editing. The ability to target the genome with precision provides new opportunities for reducing breeding time, increasing yield, and optimizing the starch properties of sweet potatoes.

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Section: Discussionmentioning

confidence: 95%