CRISPR/Cas9 alter NFYA binding site on CD44-regulating cis-element and control CD44 expression in breast cancer cells
Sohair Salem,
Shaymaa MM Yahya
Abstract:Background
CRISPR/Cas9 is used for editing of non-coding sequence. This study tested the involvement of downstream cis-element in regulating CD44 expression and the possibility of using CRISPR/Cas9 system to manipulate the transcription factor binding site. Bioinformatic tools predicted two binding sites (P1 and P2) for NFYA transcription factors. CRISPR/Cas9 was used to knockout NFYA gene and alter the NFYA binding site (P2).
Results
The data revealed decrease of CD44 gene expression and CD44+ CD24− sub-pop… Show more
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