CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists

Markossian, Suzy; Flamant, Frédéric

doi:10.1530/jme-15-0305

Cited by 9 publications

(5 citation statements)

References 120 publications

(96 reference statements)

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“…Since its adaptation to mammalian cells 1 , 2 , CRISPR/Cas9 genome editing has become a key element of the genetic toolbox in mice 3 , 4 . The method relies on Streptococcus pyogenes Cas9 (SpCas9), a RNA-guided DNA endonuclease.…”

Section: Introductionmentioning

confidence: 99%

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

Teixeira¹,

Py²,

Bosc³

et al. 2018

Sci Rep

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In this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice. The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide RNA. This simple cloning-free method proves to be extremely efficient for the generation of indels and small deletions by non-homologous end joining, and for the generation of specific point mutations by homology-directed repair. The procedure, which avoids DNA construction, in vitro transcription and oocyte microinjection, greatly simplifies genome editing in mice.

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Section: Introductionmentioning

confidence: 99%

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

Teixeira¹,

Py²,

Bosc³

et al. 2018

Sci Rep

Self Cite

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show abstract

“…While identifying novel epistatic interactions with certain disease-associated mutations may be worth the current expense of a long-term mouse breeding program, it is possible that technological advances will make searching for epistatic effects in mice more practical in the near future. For instance, as mouse CRISPR technology becomes more commonly available and efficient ( Markossian and Flamant 2016 ; Tschaharganeh et al 2016 ), it should be possible to delete a gene or introduce a mutation of interest onto existing recombinant inbred strains, like the Collaborative Cross mice. Alternatively, it may not always be necessary to introduce the mutation of interest onto all inbred backgrounds under study.…”

Section: Discussionmentioning

confidence: 99%

The Interaction of Genetic Background and Mutational Effects in Regulation of Mouse Craniofacial Shape

Percival

Marangoni

Tapaltsyan

et al. 2017

G3 Genes|Genomes|Genetics

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Inbred genetic background significantly influences the expression of phenotypes associated with known genetic perturbations and can underlie variation in disease severity between individuals with the same mutation. However, the effect of epistatic interactions on the development of complex traits, such as craniofacial morphology, is poorly understood. Here, we investigated the effect of three inbred backgrounds (129X1/SvJ, C57BL/6J, and FVB/NJ) on the expression of craniofacial dysmorphology in mice (Mus musculus) with loss of function in three members of the Sprouty family of growth factor negative regulators (Spry1, Spry2, or Spry4) in order to explore the impact of epistatic interactions on skull morphology. We found that the interaction of inbred background and the Sprouty genotype explains as much craniofacial shape variation as the Sprouty genotype alone. The most severely affected genotypes display a relatively short and wide skull, a rounded cranial vault, and a more highly angled inferior profile. Our results suggest that the FVB background is more resilient to Sprouty loss of function than either C57 or 129, and that Spry4 loss is generally less severe than loss of Spry1 or Spry2. While the specific modifier genes responsible for these significant background effects remain unknown, our results highlight the value of intercrossing mice of multiple inbred backgrounds to identify the genes and developmental interactions that modulate the severity of craniofacial dysmorphology. Our quantitative results represent an important first step toward elucidating genetic interactions underlying variation in robustness to known genetic perturbations in mice.

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“…The CRISPR system was first discovered in Escherichia coli and its role in bacterial resistance to viruses (Doudna & Charpentier, 2014;Jinek et al, 2012). CRISPR/Cas9 can site-specific binding by combining the system with endonuclease Cas9 and utilised in different non-bacterial organisms (Kotwica-Rolinska et al, 2019;Markossian & Flamant, 2016). CRISPR/Cas9 mechanism begins with the site-specific binding of a ~100-nt sequence single guide RNA (sgRNA) to the target sequence of a 5'-NGG-3' protospacer adjacent motif (PAM).…”

Section: Zinc Finger Nucleases (Zfns)mentioning

confidence: 99%

Genome Editing for the Development of Rice Resistance against Stresses: A Review

Zainuddin¹,

Mohd-Zim²,

Azmi³

et al. 2021

JTAS

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Food security is the most crucial issue faced by humans considering the rising population. Rice, a staple food consumed by nearly 50% of the world’s population, faces challenges to meet the consumers’ demand to ensure self-sufficiency amidst various abiotic and biotic stresses. Drought, salinity, heat, and infection by bacteria and viruses are the main challenges in rice cultivation. Genome editing technology provides abundant opportunities to implement selective genome modifications. Moreover, it finds the functional implications of different genome components in rice and provides a new approach for creating rice varieties tolerant of stresses. This review focuses on rice production worldwide and challenges faced in rice cultivation, and current genome editing tools available that can be utilised for crop breeding and improvement. In addition, the application of genome editing to develop biotic and abiotic resistance rice varieties is critically discussed.

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CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists

Cited by 9 publications

References 120 publications

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

The Interaction of Genetic Background and Mutational Effects in Regulation of Mouse Craniofacial Shape

Genome Editing for the Development of Rice Resistance against Stresses: A Review

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