CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases

Ortiz-Cartagena, Concha; Pablo-Marcos, Daniel; Fernández-García, Laura; Blasco, Lucía; Pacios, Olga; Bleriot, Inés; Siller, María; López, María; Fernández, Javier; Aracil, Belén; Fraile-Ribot, Pablo A; García‐Fernández, Sergio; Fernández-Cuenca, Felipe; Hernández-García, Marta; Cantón, Rafael; Calvo-Montes, Jorge; Tomás, María

doi:10.1128/spectrum.01329-23

Cited by 2 publications

(1 citation statement)

References 29 publications

(61 reference statements)

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“…This system can selectively detect single nucleotide polymorphisms (SNPs) and a variety of targets such as viral RNA, human cell mRNA, and circular RNA. Ortiz-Cartagena et al (2023) developed a precise detection technique utilizing LAMP and CRISPR-Cas13a, effectively identifying OXA-48 and GES Carbapenemases in various clinical specimens, with sensitivity and specificity levels reaching 100%. Wang Q. et al (2024) introduced a Cas14a1-enhanced LAMP assay in conjunction with Rapid Extraction of Bacterial Genomic DNA, achieving the LOD of 10 aM target genomic DNA.…”

Section: Combination Of Lamp With Other Techniquesmentioning

confidence: 99%

Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview

Yang,

Zhang,

Han

et al. 2024

Front. Microbiol.

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Loop-mediated isothermal amplification (LAMP) is a novel method for nucleic acid detection known for its isothermal properties, high efficiency, sensitivity, and specificity. LAMP employs 4 to 6 primers targeting 6 to 8 regions of the desired sequence, allowing for amplification at temperatures between 60 and 65°C and the production of up to 109 copies within a single hour. The product can be monitored by various methods such as turbidimetry, fluorometry, and colorimetry. However, it faces limitations such as the risk of non-specific amplification, challenges in primer design, unsuitability for short gene sequences, and difficulty in multiplexing. Recent advancements in polymerase and primer design have enhanced the speed and convenience of the LAMP reaction. Additionally, integrating LAMP with technologies like rolling circle amplification (RCA), recombinase polymerase amplification (RPA), and CRISPR-Cas systems has enhanced its efficiency. The combination of LAMP with various biosensors has enabled real-time analysis, broadening its application in point-of-care testing (POCT). Microfluidic technology has further facilitated the automation and miniaturization of LAMP assays, allowing for the simultaneous detection of multiple targets and preventing contamination. This review highlights advancements in LAMP, focusing on primer design, polymerase engineering, and its integration with other technologies. Continuous improvements and integration of LAMP with complementary technologies have significantly enhanced its diagnostic capabilities, making it a robust tool for rapid, sensitive, and specific nucleic acid detection with promising implications for healthcare, agriculture, and environmental monitoring.

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Section: Combination Of Lamp With Other Techniquesmentioning

confidence: 99%