CRISPR/Cas13a assisted amplification of magnetic relaxation switching sensing for accurate detection of miRNA-21 in human serum

Ultrasensitive miRNA Detection Based on Magnetic Upconversion Nanoparticle Enhancement and CRISPR/Cas13a-Driven Signal Amplification

“…The correlation equation was Y = 1673X − 2350, R 2 = 0.9846, and the detection limit was calculated to be 83.2 fM. Compared with previous studies of miRNA detection based on CRISPR/Cas13a, 38,39 the sensitivity was significantly improved.…”

Application of CRISPR/Cas13a-based biosensors in serum marker detection

“…When the magnetic nanoparticles specifically bind with the object to be detected, they change from a dispersed state to an aggregated state, which changes the transverse relaxation time (T2) of water protons, thus realizing the detection of the object to be detected. 91 Guo et al 92 constructed a biosensor combining a magnetic relaxation switch (MRS) with Cas13a, which can directly detect miR-21 within 60 minutes without extraction, and the detection limit is 0.22 pM. The signal probe (MB30-RNA1–MM1000) was constructed by labeling ssRNA(RNA1) with carboxyl modified 30 nm magnetic nanoparticles (MB30) and 1000 nm diameter streptavidin-modified magnetic beads (MM1000).…”

Advanced technologies towards improved HPV diagnostics

“…Currently, there is a plethora of different caspases, for example, Cas9, Cas12, Cas13, Cas14, and their subtypes, 106,107 that specifically cleave various target molecules such as single‐stranded (ss) DNA, double‐stranded (ds) DNA, ss RNA with cis‐ and/or trans‐cleavage activity, 108 or even unwind dsDNA 109 . Accordingly, CRISPR‐Cas system has been employed in detection of wide range of biomarkers, including microRNAs, 110,111 DNA methylation, 112 single point mutations, 113,114 various bacteria such as Salmonella , 115,116 Yersinia pestis , 117 or viruses such as SARS‐CoV‐2, 118 EBV, 119 as well as HPV 120–142 . Interestingly, CRISPR‐Cas technology was recently used also in a treatment of HPV infection‐associated cervical cancer.…”