CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid

Abstract

This work reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for quantification of human papillomavirus (HPV)-related DNA by...

CRISPR-Cas12a-mediated label-free electrochemical aptamer-based sensor for SARS-CoV-2 antigen detection

“…Recently, CRISPR-Cas has emerged as a powerful technology that is revolutionizing next-generation diagnostic platforms [6] , [7] . The mechanism of CRISPR-Cas systems, which are programmed by CRISPR guide RNA (crRNA), has been reported to not only cleave the target nucleic acid but also cleave all neighbouring nucleic acids indiscriminately [8] , [9] . This target-triggered collateral activity of CRISPR-Cas systems has stimulated the development of CRISPR-based biosensing technologies [10] , [11] , which offer unique advantages of simple fabrication, ultrahigh sensitivity, high specificity to single-base variation, and good amenability for POC diagnostics [12] , [13] , [14] .…”

Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids

“…10,11 Furthermore, diagnostic technologies based on the CRISPR-associated nuclease (Cas) system have also been developed rapidly. [12][13][14][15] The CRISPR-Cas system has many notable advantages, like mild reaction temperature, fast reaction time, excellent specificity, and highefficiency signal amplification capabilities. These features make it a powerful next-generation diagnostic technology.…”

CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

“…21,22 Afterward, newer methods were introduced by utilizing the sequence-nonspecific collateral cleavage activity found in Cas12 and Cas13 proteins that has facilitated the development of numerous diagnostic tools for rapid and portable detection, consequently yielding great potential for point of care settings. 23–26 Beyond nucleic acid detection, 27–29 Cas12a is being recently exploited to construct novel strategies for the detection of various biological analytes such as small molecules, 30 lipopolysaccharide, 31 transcription factors, 32 and enzyme activities 33 due to its exceptional capability for signal amplification by cleaving nearby ssDNA reporter upon target DNA recognition.…”