CRISPR/Cas12a-mediated electrochemiluminescence platform for environmental and human serum SARS-CoV-2 RNA monitoring using a self-enhanced ruthenium complex linked to zeolitic imidazole framework-8

Abstract

Since the emergence of coronavirus disease 2019 (COVID-19), this high-contagious disease has ravaged the world, cumulatively causing millions of deaths and huge economic losses. As the culprit of COVID-19, severe...

Electrochemiluminescence Biosensor for MMP-2 Analysis Using CRISPR/Cas13a and EXPAR Amplification: A Novel Approach for Anti-Aging Research

“…Class II systems, such as Cas9, Cas12, and Cas13, are particularly notable for their broad applications due to their simpler composition, consisting of a single effector protein and a programmable guide RNA. [19][20][21][22][23][24][25] Recent advancements in gene editing have led to notable discoveries, including new genome editing techniques. Speci cally, the trans-cleavage activity discovered in Cas12a, Cas13a, and Cas14a has positioned the CRISPR/Cas system as a promising candidate for next-generation diagnostic biosensing platforms.…”

“…Electrochemiluminescence (ECL) is a chemiluminescence technique that utilizes electrochemical stimulation by applying a voltage or electric current to the surface of an electrode or an electrolyte to trigger a exothermic redox reaction in a substance, resulting in the production of molecules or free radicals in the excited state and the release of photons as they return to their ground state. [22,28,42] This process has a number of advantages. First, the background noise is very low because only substances in which exothermic redox reactions are present can emit light, excluding the in uence of other interfering substances.…”