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Note: Selection based on URA3 / URA5 has not been established in M. oryzae , and preliminary experiments might be needed to confirm selection based on URA3 / URA5 in M. oryzae .…”
Note: Selection based on URA3 / URA5 has not been established in M. oryzae , and preliminary experiments might be needed to confirm selection based on URA3 / URA5 in M. oryzae .…”
Note: If your gene of interest can be used as selection directly, DNA donor is not required in step 22 For example, we have generated fkbp12 (FK506 receptor) mutants by using the selection of drug FK506 without DNA donor successfully ( Huang et al., 2021 ).