CRISPR-Cas12a bends DNA to destabilize base pairs during target interrogation
Katarzyna M. Soczek,
Joshua C. Cofsky,
Owen T. Tuck
et al.
Abstract:RNA-guided endonucleases are involved in processes ranging from adaptive immunity to site-specific transposition and have revolutionized genome editing. CRISPR-Cas9, -Cas12 and related proteins use guide RNAs to recognize ∼20-nucleotide target sites within genomic DNA by mechanisms that are not yet fully understood. We used structural and biochemical methods to assess early steps in DNA recognition by Cas12a protein-guide RNA complexes. We show here that Cas12a initiates DNA target recognition by bending DNA t… Show more
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