CRISPR/Cas12a-Amplified Aptamer Switch Microplate Assay for Small Molecules
Fengxi Zhu,
Hao Yu,
Qiang Zhao
Abstract:The presence of small molecule contaminants
such as mycotoxins
and heavy metals in foods and the environment causes a serious threat
to human health and huge economic losses. The development of simple,
rapid, sensitive, and on-site methods for small molecule pollutant
detection is highly demanded. Here, combining the advantages of structure-switchable
aptamer-mediated signal conversion and CRISPR/Cas12a-based signal
amplification, we developed a CRISPR/Cas12a-amplified aptamer switch
assay on a microplate for … Show more
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