CRISPR-Cas system as a promising player against bacterial infection and antibiotic resistance

Abavisani, Mohammad; Khayami, Reza; Hoseinzadeh, Melika; Kodori, Mansoor; Kesharwani, Prashant; Sahebkar, Amirhossein

doi:10.1016/j.drup.2023.100948

Cited by 12 publications

(7 citation statements)

References 208 publications

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“…RM systems function by cleaving foreign DNA, serving as an innate defense mechanism. In contrast, the CRISPR-Cas system has garnered significant attention due to its capacity to manipulate bacterial genetic elements and its potential associations with antibiotic resistance genes (ARGs) in various species [ 76 – 78 ]. However, it's important to note that these defense mechanisms do not entirely block horizontal gene transfer; instead, they selectively inhibit the uptake of foreign genetic elements [ 13 , 79 ].…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Ahmed,

Khattab,

Ragab

et al. 2023

BMC Genomics

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Background The gut microbiota is considered a rich source for potential novel probiotics. Enterococcus genus is a normal component of a healthy gut microbiota, suggesting its vital role. Nosocomial infections caused mainly by E. facalis and E. faecium have been attributed to the plasticity of the Enterococcus genomes. In this study, we assessed the probiotic and safety characteristics of two E. lactis strains isolated from the human gut microbiota using in-vitro and in silico approaches. Additionally, the safety of the E. lactis species was evaluated using comparative genomics analysis. Results The two E. lactis strains 10NA and 50NA showed resistance to bile salts and acid tolerance with antibacterial activity against Escherichia coli, Salmonella typhi, and Clostridioides difficile. For safety assays, the two strains did not display any type of hemolysis on blood agar, and the survival of Caco-2 cells was not significantly different (P-value > 0.05) compared to the control using cell free supernatants at 100% (v/v), 50% (v/v), 10% (v/v), and 5% (v/v) concentrations. Regarding antibiotic susceptibility, both strains were sensitive to vancomycin, tetracycline, and chloramphenicol. Comprehensive whole-genome analysis revealed no concerning associations between virulence or antibiotic resistance genes and any of the identified mobile genetic elements. Comparative genome analysis with closely related E. faecium species genomes revealed the distinctive genomic safety of the E. lactis species. Conclusions Our two E. lactis strains showed promising probiotic properties in-vitro. Their genomes were devoid of any transferable antibiotic resistance genes. In silico comparative analysis confirmed the safety of the E. lactis species. These results suggest that E. lactis species could be a potential source for safer Enterococcus probiotic supplements.

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Section: Discussionmentioning

confidence: 99%

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Ahmed,

Khattab,

Ragab

et al. 2023

BMC Genomics

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“…Some published articles report key factors of QS signaling where tools such as CRISPR Cas-9, dCas9, 12a, 13, 13a, and 14 a1 can be used to regulate the virulent effects of bacteria. Kiga et al 2020, demonstrated the potential and effectiveness of the use of CRISPR-type techniques to promote useful strategies for the regulation of signaling in pathogenic microorganisms such as E. coli and S. aureus and achieve effective QQ [57,58], while the studies of Sharma et al…”

Section: Cd01374 Rjf 004047 Km101117mentioning

confidence: 99%

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Higuera-Ciapara,

Benitez-Vindiola,

Figueroa-Yañez

et al. 2024

Foods

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Antibiotic resistance in foodborne pathogens is an increasing threat to global human health. Among the most prevalent antibiotic-resistant bacteria are Salmonella enterica serovar Typhimurium, Campylobacter jejuni and E. coli 0157:H7. Control of these and other pathogens requires innovative approaches, i.e., discovering new molecules that will inactivate them, or render them less virulent without inducing resistance. Recently, several polyphenol molecules have been shown to possess such characteristics. Also, the use of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) approaches has recently been proposed for such purpose. This review summarizes the main findings regarding the application of both approaches to control the above-mentioned foodborne pathogens by relying on Quorum Sensing interference (Quorum Quenching) mechanisms and highlights the avenues needed for further research.

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“…Craspase can not only be used to target a specific RNA and/or protein for CRISPR gene therapy, but also has the potential to diagnose and treat infectious diseases (Fig. 1 B), such as HIV and COVID-19, similarly to Cas9/Cas12 (Abavisani et al 2023 ; Zhou et al 2022 ). By deactivating Craspase through removing its RNase and protease activities but keeping its binding functions, it can be used for imaging of targeting RNA and protein sequences in human cells, particularly pathological cells such as cancer cells (Fig.…”

Section: Craspase Has Great Application Potentials In Gene Therapy An...mentioning

confidence: 99%

Craspase: A novel CRISPR/Cas dual gene editor

Huo

Shepherd

Pan

2023

Funct Integr Genomics

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CRISPR-Cas system as a promising player against bacterial infection and antibiotic resistance

Cited by 12 publications

References 208 publications

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Craspase: A novel CRISPR/Cas dual gene editor

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