CRISPR/Cas-mediated genome editing for improved stress tolerance in plants

Pandita, Deepu

doi:10.1016/b978-0-323-90943-3.00001-8

Cited by 7 publications

(2 citation statements)

References 206 publications

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“…All these reports strongly suggest that genetic engineering technology speeds up the development of stress-resilient crop plants for food and nutritional security. The last few decades have witnessed shifting research on genome-editing technologies, such as ZFN, TALEN, and CRISPR/Cas systems, for higher accuracy and precision to develop desired crop plants with tolerance to different abiotic stressors ( Mushtaq et al, 2021 ; Pandita, 2021 ). For instance, Li et al (2019) employed CRISPR/Cas9 to mediate mutagenesis of SlNPR1 to enhance drought tolerance in tomatoes.…”

Section: High-throughput Approaches To Investigate Si-induced Toleran...mentioning

confidence: 99%

Multidimensional Role of Silicon to Activate Resilient Plant Growth and to Mitigate Abiotic Stress

et al. 2022

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Sustainable agricultural production is critically antagonistic by fluctuating unfavorable environmental conditions. The introduction of mineral elements emerged as the most exciting and magical aspect, apart from the novel intervention of traditional and applied strategies to defend the abiotic stress conditions. The silicon (Si) has ameliorating impacts by regulating diverse functionalities on enhancing the growth and development of crop plants. Si is categorized as a non-essential element since crop plants accumulate less during normal environmental conditions. Studies on the application of Si in plants highlight the beneficial role of Si during extreme stressful conditions through modulation of several metabolites during abiotic stress conditions. Phytohormones are primary plant metabolites positively regulated by Si during abiotic stress conditions. Phytohormones play a pivotal role in crop plants’ broad-spectrum biochemical and physiological aspects during normal and extreme environmental conditions. Frontline phytohormones include auxin, cytokinin, ethylene, gibberellin, salicylic acid, abscisic acid, brassinosteroids, and jasmonic acid. These phytohormones are internally correlated with Si in regulating abiotic stress tolerance mechanisms. This review explores insights into the role of Si in enhancing the phytohormone metabolism and its role in maintaining the physiological and biochemical well-being of crop plants during diverse abiotic stresses. Moreover, in-depth information about Si’s pivotal role in inducing abiotic stress tolerance in crop plants through metabolic and molecular modulations is elaborated. Furthermore, the potential of various high throughput technologies has also been discussed in improving Si-induced multiple stress tolerance. In addition, a special emphasis is engrossed in the role of Si in achieving sustainable agricultural growth and global food security.

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Section: High-throughput Approaches To Investigate Si-induced Toleran...mentioning

confidence: 99%

Multidimensional Role of Silicon to Activate Resilient Plant Growth and to Mitigate Abiotic Stress

et al. 2022

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“…The CRISPR–Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9) system has emerged as an innovative gene editing tool in plant systems ( Venegas-Rioseco et al., 2021 ). It is a quick, cost friendly tool that can be used for improving crop traits against abiotic and biotic stress tolerance ( Pandita, 2021 ). It consists of spacer sequences placed between short palindromic repeats which transcribe to CRISPR RNA (crRNA), which combines with transactivating crRNA (tracrRNA) to form a mature crRNA/tracrRNA complex (guide RNA/gRNA).…”

Section: Various Approaches To Ameliorate Heavy Metal Stress In Medic...mentioning

confidence: 99%

Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

et al. 2023

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Medicinal plants, an important source of herbal medicine, are gaining more demand with the growing human needs in recent times. However, these medicinal plants have been recognized as one of the possible sources of heavy metal toxicity in humans as these medicinal plants are exposed to cadmium-rich soil and water because of extensive industrial and agricultural operations. Cadmium (Cd) is an extremely hazardous metal that has a deleterious impact on plant development and productivity. These plants uptake Cd by symplastic, apoplastic, or via specialized transporters such as HMA, MTPs, NRAMP, ZIP, and ZRT-IRT-like proteins. Cd exerts its effect by producing reactive oxygen species (ROS) and interfere with a range of metabolic and physiological pathways. Studies have shown that it has detrimental effects on various plant growth stages like germination, vegetative and reproductive stages by analyzing the anatomical, morphological and biochemical changes (changes in photosynthetic machinery and membrane permeability). Also, plants respond to Cd toxicity by using various enzymatic and non-enzymatic antioxidant systems. Furthermore, the ROS generated due to the heavy metal stress alters the genes that are actively involved in signal transduction. Thus, the biosynthetic pathway of the important secondary metabolite is altered thereby affecting the synthesis of secondary metabolites either by enhancing or suppressing the metabolite production. The present review discusses the abundance of Cd and its incorporation, accumulation and translocation by plants, phytotoxic implications, and morphological, physiological, biochemical and molecular responses of medicinal plants to Cd toxicity. It explains the Cd detoxification mechanisms exhibited by the medicinal plants and further discusses the omics and biotechnological strategies such as genetic engineering and gene editing CRISPR- Cas 9 approach to ameliorate the Cd stress.

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Unlocking CRISPR/Cas-Mediated Editing Potential for Designing Climate-Smart Crop Plants

Pandita

2023

Climate-Resilient Agriculture, Vol 1

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CRISPR/Cas-mediated genome editing for improved stress tolerance in plants

Cited by 7 publications

References 206 publications

Multidimensional Role of Silicon to Activate Resilient Plant Growth and to Mitigate Abiotic Stress

Multidimensional Role of Silicon to Activate Resilient Plant Growth and to Mitigate Abiotic Stress

Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

Unlocking CRISPR/Cas-Mediated Editing Potential for Designing Climate-Smart Crop Plants

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