CRISPR/Cas genome editing improves abiotic and biotic stress tolerance of crops

Li, Yangyang; Wu, Xiuzhe; Zhang, Yan; Zhang, Qiang

doi:10.3389/fgeed.2022.987817

Cited by 29 publications

(14 citation statements)

References 133 publications

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“…Figure 3 demonstrated these negative regulators of the stress-responsive genes and their inhibitors by editing them to improve stress tolerance for salinity, drought, cold, etc. In addition to the knockout and allele insertion-deletion, the CRISPR editing tool also generates tolerant plants by modulating the genomic aspects through transcript activation, epigenetic modification by chromatin unwinding, and swapping promoter regions with HDR (Li X et al, 2022). These Arabidopsis mutant plants were epigenetically modified for ABA/AREB1 responsive transcription factor by chromatin unwinding, contributing to improving drought tolerance (Roca Paixão et al, 2019).…”

Section: Mechanism For Abiotic Stress Tolerancementioning

confidence: 99%

“…Due to its simplicity, efficiency, and versatility, CRISPR/Cas9-mediated genome editing has currently become a prevalent technology and has been extensively used to develop resistance in crop plants ( Wolter et al., 2019 ). It can be used for knockout, replacement and insertion of gene that resulted in loss-of-function, knock down or activation mutants, that can lead to development of abiotic/biotic stress-tolerant crop plants ( Li Y et al., 2022 ). Application of multiplex genome editing would open the ways for the development of genome-edited crops engineered for tolerance against multiple traits in a single transformation event.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

et al. 2023

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Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches and transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery of engineered nucleases as genetic scissors to carry out precise manipulation in crop stress-responsive genes and associated molecular network has paved the way for sustainable management of abiotic stress conditions. In this context, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool has revolutionized due to its simplicity, accessibility, adaptability, flexibility, and wide applicability. This system has great potential to build up crop varieties with enhanced tolerance against abiotic stresses. In this review, we summarize the latest findings on understanding the mechanism of abiotic stress response in plants and the application of CRISPR/Cas-mediated gene-editing system towards enhanced tolerance to a multitude of stresses including drought, salinity, cold, heat, and heavy metals. We provide mechanistic insights on the CRISPR/Cas9-based genome editing technology. We also discuss applications of evolving genome editing techniques such as prime editing and base editing, mutant library production, transgene free and multiplexing to rapidly deliver modern crop cultivars adapted to abiotic stress conditions.

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Section: Mechanism For Abiotic Stress Tolerancementioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

et al. 2023

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“…Due to regulatory hurdles the transgenic breeding approaches has not been widely used. Under such a scenario the CRISPR/Cas9 technology is gaining traction in crop breeding and genetic improvement of many targeted traits including abiotic stress tolerance in many crop species ( Li et al., 2022a ). However, there is limited research on peas and other legumes which need attention.…”

Section: Genomics For Hs In Pisummentioning

confidence: 99%

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

Devi

Sagar²,

Mishra³

et al. 2023

Front. Plant Sci.

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In the era of climate change, the overall productivity of pea (Pisum sativum L.) is being threatened by several abiotic stresses including heat stress (HS). HS causes severe yield losses by adversely affecting several traits in peas. A reduction in pod yield has been reported from 11.1% to 17.5% when mean daily temperature increase from 1.4 to 2.2°C. High-temperature stress (30.5-33°C) especially during reproductive phase is known to drastically reduce both seed yield and germination. HS during germination and early vegetative stage resulted in poor emergence and stunted plant growth along with detrimental effects on physiological functions of the pea plant. To combat HS and continue its life cycle, plants use various defense strategies including heat escape, avoidance or tolerance mechanisms. Ironically, the threshold temperatures for pea plant and its responses are inconsistent and not yet clearly identified. Trait discovery through traditional breeding such as semi leaflessness (afila), upright growing habit, lodging tolerance, lower canopy temperature and small seeded nature has highlighted their utility for greater adaptation under HS in pea. Screening of crop gene pool and landraces for HS tolerance in a targeted environment is a simple approach to identify HS tolerant genotypes. Thus, precise phenotyping using modern phenomics tools could lead to increased breeding efficiency. The NGS (next generation sequencing) data can be associated to find the candidate genes responsible for the HS tolerance in pea. In addition, genomic selection, genome wide association studies (GWAS) and marker assisted selection (MAS) can be used for the development of HS tolerant pea genotypes. Additionally, development of transgenics could be an alternative strategy for the development of HS tolerant pea genotypes. This review comprehensively covers the various aspects of HS tolerance mechanisms in the pea plant, screening protocols, omic advances, and future challenges for the development of HS tolerant genotypes.

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“…Although the CRISPR/Cas systems exhibit powerful ability in crop genetic improvement, some limitations still need to be overcome in this field [ 195 , 196 , 197 , 198 , 199 , 200 ]. SpCas9 requires a 5′-NGG-3ʹPAM immediately adjacent to the 20 nt DNA target sequence because it can only recognize NGG PAM sites, which limits its effectiveness.…”

Section: Crispr/cas Systems Of Advances Limitations and Prospective A...mentioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game-changing tool that can create variation in desired traits, such as biotic and abiotic resistance, increase quality and yield in less time with easy applications, high efficiency, and low cost in producing the targeted edits for rapid improvement of crop plants. Plant pathogens and the severe environment cause considerable crop losses worldwide. GE approaches have emerged and opened new doors for breeding multiple-resistance crop varieties. Here, we have summarized recent advances in CRISPR/Cas-mediated GE for resistance against biotic and abiotic stresses in a crop molecular breeding program that includes the modification and improvement of genes response to biotic stresses induced by fungus, virus, and bacterial pathogens. We also discussed in depth the application of CRISPR/Cas for abiotic stresses (herbicide, drought, heat, and cold) in plants. In addition, we discussed the limitations and future challenges faced by breeders using GE tools for crop improvement and suggested directions for future improvements in GE for agricultural applications, providing novel ideas to create super cultivars with broad resistance to biotic and abiotic stress.

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CRISPR/Cas genome editing improves abiotic and biotic stress tolerance of crops

Cited by 29 publications

References 133 publications

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

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