CRISPR/Cas‐based precision genome editing via microhomology‐mediated end joining

Vu, Tien Van; Doan, Duong; Kim, Ji-Hae; Sung, Yeon Woo; Tran, Mil Thi; Song, Yadong; Das, Swati; Kim, Jae‐Yean

doi:10.1111/pbi.13490

Cited by 28 publications

(13 citation statements)

References 118 publications

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“…Error-prone NHEJ is designed for gene knockout by disrupting the coding sequence of that target gene due to the introduction of random indels or frameshift mutations while HDR and HR repairs are programmable and their effects are predictable. A more precise error-prone repair mechanism alternative to HR, microhomology-mediated end joining (MHEJ), has been believed to enhance the efficiency of genome editing in plants [ 38 ]. The mechanism of TALEN-based genome-editing is basically via the disruption of the effector-binding element of the S gene promoter which eventually impairs the compatible molecular interactions between the effector and the target S gene.…”

Section: Genome-editing: Mechanisms and Variant Toolsmentioning

confidence: 99%

Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selected Solanaceae crop plants

Barka

Lee

2022

Bioengineered

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Genome-editing tools for the development of traits to tolerate abiotic and biotic adversaries are the recently devised breeding techniques revolutionizing molecular breeding by addressing the issues of rapidness and precision. To that end, disease resistance development by disrupting disease susceptibility genes (S genes) to intervene in the biological mechanism of pathogenicity has significantly improved the techniques of molecular breeding. Despite the achievements in genome-editing aimed at the intervention of the function of susceptibility determinants or gene regulatory elements, off-target effects associated with yield-related traits are still the main setbacks. The challenges are attributed to the complexity of the inheritance of traits controlled by pleiotropic genes. Therefore, a more rigorous genome-editing tool with ultra-precision and efficiency for the development of broad-spectrum and durable disease resistance applied to staple crop plants is of critical importance in molecular breeding programs. The main objective of this article is to review the most impressive progresses achieved in resistance breeding against the main diseases of three Solanaceae crops (potato, Solanum tuberosum ; tomato, Solanum lycopersicum and pepper, Capsicum annuum ) using genome-editing by disrupting the sequences of S genes, their promoters, or pathogen genes. In this paper, we discussed the complexity and applicability of genome-editing tools, summarized the main disease of Solanaceae crops, and compiled the recent reports on disease resistance developed by S-gene silencing and their off-target effects. Moreover, GO count and gene annotation were made for pooled S-genes from biological databases. Achievements and prospects of S-gene-based next-generation breeding technologies are also discussed.

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Section: Genome-editing: Mechanisms and Variant Toolsmentioning

confidence: 99%

Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selected Solanaceae crop plants

Barka

Lee

2022

Bioengineered

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“…The mutant efficiency of MMEJ is similar to the NHEJ mechanism, which show a high potential for precise gene editing compared with HDR ( Seol et al, 2018 ). Yao and co-workers showed that the mutation frequencies were as high as 20% by using MMEJ-mediated knock-in, which was approximately 10-fold higher than the HDR-based approach ( Van Vu et al, 2021 ).…”

Section: The Mechanism Of Crispr-cas9 Systemmentioning

confidence: 99%

The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System

Qiao

Zhang

et al. 2021

Front. Chem.

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The study of different genes, chromosomes and the spatiotemporal relationship between them is of great significance in the field of biomedicine. CRISPR-Cas9 has become the most widely used gene editing tool due to its excellent targeting ability. In recent years, a series of advanced imaging technologies based on Cas9 have been reported, providing fast and convenient tools for studying the sites location of genome, RNA, and chromatin. At the same time, a variety of CRISPR-Cas9-based imaging systems have been developed, which are widely used in real-time multi-site imaging in vivo. In this review, we summarized the component and mechanism of CRISPR-Cas9 system, overviewed the NIR imaging and the application of NIR fluorophores in the delivery of CRISPR-Cas9, and highlighted advances of the CRISPR-Cas9-based imaging system. In addition, we also discussed the challenges and potential solutions of CRISPR-Cas9-based imaging methods, and looked forward to the development trend of the field.

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“…These editors have the distinct advantage of inducing simultaneous mutations at C > T and A > G target sites under the guidance of a single sgRNA. Moreover, the saturation degree and the diversity of mutation types of the target gene were increased significantly using this technology [103][104][105][106][107][108].…”

Section: Double Base Editing Techniquesmentioning

confidence: 99%