CRISPR-Cas-based plant genome engineering goes viral

Daròs, José‐Antonio; Pasin, Fabio; Merwaiss, Fernando

doi:10.1016/j.molp.2023.03.010

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…More recently, viral vectors have been used to express the molecular components required for genome editing, such as sequence-specific nucleases and guide RNAs, or to introduce repair templates for targeted genome editing, also called virus-induced genome editing (VIGE) [2, 5,6]. Such viral vectors can be used to introduce targeted genome modifications in planta, thus avoiding the transformation of isolated single cells or plant tissue in vitro [7,8].…”

Section: Introductionmentioning

confidence: 99%

Scanning the Horizon for Environmental Applications of Genetically Modified Viruses Reveals Challenges for Their Environmental Risk Assessment

Eckerstorfer,

Dolezel,

Miklau

et al. 2024

IJMS

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The release of novel genetically modified (GM) virus applications into the environment for agricultural, veterinary, and nature-conservation purposes poses a number of significant challenges for risk assessors and regulatory authorities. Continuous efforts to scan the horizon for emerging applications are needed to gain an overview of new GM virus applications. In addition, appropriate approaches for risk assessment and management have to be developed. These approaches need to address pertinent challenges, in particular with regard to the environmental release of GM virus applications with a high probability for transmission and spreading, including transboundary movements and a high potential to result in adverse environmental effects. However, the current preparedness at the EU and international level to assess such GM virus application is limited. This study addresses some of the challenges associated with the current situation, firstly, by conducting a horizon scan to identify emerging GM virus applications with relevance for the environment. Secondly, outstanding issues regarding the environmental risk assessment (ERA) of GM virus applications are identified based on an evaluation of case study examples. Specifically, the limited scientific information available for the ERA of some applications and the lack of detailed and appropriate guidance for ERA are discussed. Furthermore, considerations are provided for future work that is needed to establish adequate risk assessment and management approaches.

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Section: Introductionmentioning

confidence: 99%

Scanning the Horizon for Environmental Applications of Genetically Modified Viruses Reveals Challenges for Their Environmental Risk Assessment

Eckerstorfer,

Dolezel,

Miklau

et al. 2024

IJMS

View full text Add to dashboard Cite

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Advances in Delivery of CRISPR–Cas Reagents for Precise Genome Editing in Plants

Yau,

Easterling,

Kumar

2024

Gene Editing in Plants

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Trans‐complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector

Huang,

Zhang,

Xiao

et al. 2024

Plant Biotechnology Journal

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SummaryThe development of plant virus‐based expression systems has expanded rapidly owing to their potential applications in gene functional and disease resistance research, and industrial production of pharmaceutical proteins. However, the low yield of certain proteins, especially high‐molecular‐mass proteins, restricts the production scale. In this study, we observed that the tobacco mosaic virus (TMV)‐mediated expression of a foreign protein was correlated with the amount of the movement protein (MP) and developed a TMV‐derived pAT‐transMP vector system incorporating trans‐complementation expression of MP. The system is capable of efficient expression of exogenous proteins, in particular those with a high molecular mass, and enables simultaneous expression of two target molecules. Furthermore, viral expression of competent CRISPR‐Cas9 protein and construction of CRISPR‐Cas9‐mediated gene‐editing system in a single pAT‐transMP construct was achieved. The results demonstrated a novel role for TMV‐MP in enhancing the accumulation of a foreign protein produced from the viral vector or a binary expression system. Further investigation of the mechanism underlying this role will be beneficial for optimization of plant viral vectors with broad applications.

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CRISPR-Cas-based plant genome engineering goes viral

Cited by 4 publications

References 10 publications

Scanning the Horizon for Environmental Applications of Genetically Modified Viruses Reveals Challenges for Their Environmental Risk Assessment

Scanning the Horizon for Environmental Applications of Genetically Modified Viruses Reveals Challenges for Their Environmental Risk Assessment

Advances in Delivery of CRISPR–Cas Reagents for Precise Genome Editing in Plants

Trans‐complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector

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