CRISPR/Cas advancements for genome editing, diagnosis, therapeutics, and vaccine development for Plasmodium parasites, and genetic engineering of Anopheles mosquito vector

Nourani, Leila; Mehrizi, Akram Abouie; Pirahamdi, Sakineh; Pourhashem, Zeinab; Asadollahi, Elahe; Jahangiri, Babak

doi:10.1016/j.meegid.2023.105419

Cited by 4 publications

(3 citation statements)

References 172 publications

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“…Genetic systems that distort sex ratios with a bias for males via RNA-guided shredding of the X-chromosome during spermatogenesis are also quite promising [ 198 , 199 , 200 ]. More current cage studies have been described, and the results lend support to the efficacy of this emerging technology in controlling vector mosquito populations [ 201 , 202 , 203 , 204 , 205 ]. In addition, commentaries on the prospect of eradicating malaria using CRISPR/Cas9 gene drive [ 206 , 207 ] and regulatory and policy considerations [ 208 ] are the subjects of scrutiny and debate.…”

Section: Effective “Green” Technologiesmentioning

confidence: 96%

The Perpetual Vector Mosquito Threat and Its Eco-Friendly Nemeses

Miranda,

Rudd,

Mena

et al. 2024

Biology

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Mosquitoes are the most notorious arthropod vectors of viral and parasitic diseases for which approximately half the world’s population, ~4,000,000,000, is at risk. Integrated pest management programs (IPMPs) have achieved some success in mitigating the regional transmission and persistence of these diseases. However, as many vector-borne diseases remain pervasive, it is obvious that IPMP successes have not been absolute in eradicating the threat imposed by mosquitoes. Moreover, the expanding mosquito geographic ranges caused by factors related to climate change and globalization (travel, trade, and migration), and the evolution of resistance to synthetic pesticides, present ongoing challenges to reducing or eliminating the local and global burden of these diseases, especially in economically and medically disadvantaged societies. Abatement strategies include the control of vector populations with synthetic pesticides and eco-friendly technologies. These “green” technologies include SIT, IIT, RIDL, CRISPR/Cas9 gene drive, and biological control that specifically targets the aquatic larval stages of mosquitoes. Regarding the latter, the most effective continues to be the widespread use of Lysinibacillus sphaericus (Ls) and Bacillus thuringiensis subsp. israelensis (Bti). Here, we present a review of the health issues elicited by vector mosquitoes, control strategies, and lastly, focus on the biology of Ls and Bti, with an emphasis on the latter, to which no resistance has been observed in the field.

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Section: Effective “Green” Technologiesmentioning

confidence: 96%

The Perpetual Vector Mosquito Threat and Its Eco-Friendly Nemeses

Miranda,

Rudd,

Mena

et al. 2024

Biology

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“…populations or the ability of vectors to transmit disease pathogens, such as viruses, bacteria, and other fungi and parasites [16,69].…”

Section: Crispr-cas9-based Interventions Have the Potential To Modify...mentioning

confidence: 99%

“…This approach offers the potential to discover new traditional medicine candidates with improved efficacy, safety, and sustainability for the treatment of zoonotic diseases in Indonesia [35,103]. CRISPR-Cas9 technology can be used for vaccine development against zoonotic diseases [16]. CRISPR technology can be used to precisely engineer vaccine candidates by modifying the pathogen genome to increase immunogenicity or attenuate virulence.…”

Section: The Use Of Crispr-cas9 In the Development Of Vaccines Tradit...mentioning

confidence: 99%

CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review

Adnyana,

Eljatin,

Sudaryati

et al. 2024

JMHT

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The increasing emergence of zoonotic diseases originating from vectors, rodents, mammals, and others has increased the potential for outbreaks of pandemics in the Indonesian territory. Although control and prevention have been implemented, these efforts have not yet revealed a bright spot; therefore, elaboration with advanced CRISPR-Cas9 technology is a way to accelerate efforts to control zoonotic diseases in Indonesia. However, there is limited literature on this topic. This review aims to comprehensively describe, identify, and summarize the application of CRISPR-Cas9 genome-editing technology in zoonotic disease control in Indonesia. Our findings show that CRISPR-Cas9 genome editing technology offers an innovative approach for zoonotic disease control by targeting disease vectors, modifying animal reservoirs, improving disease surveillance, enhancing vaccine development, and exploring traditional medicine candidates and immunotherapy. The high level of precision, efficiency, and versatility in targeting genomes capable of disrupting, damaging, and disrupting the disease transmission cycle in pathogens makes CRISPR-Cas9 highly effective. However, challenges such as off-target impacts, regulatory complexity, and ethical considerations must be overcome with inter- and multidisciplinary collaboration to promote transparency, equity, and public engagement throughout the process of implementing this technology in the field, especially in Indonesia.

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