CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens

Rahman, Md. Rashidur; Majumder, Toma Rani; Apu, Md. Aminul Islam; Paul, Alok Kumar; Afrose, Afrina; Dash, Biplab Kumar

doi:10.1155/2022/5390685

Cited by 3 publications

(3 citation statements)

References 109 publications

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“…INAAT techniques are ideally suited for adaptation to POC and field deployable settings [ 89 ]. A wide range of technologies have been developed as promising candidates for POC devices which could be used in combination with INAAT and are briefly discussed below.…”

Section: Point Of Care Diagnosticsmentioning

confidence: 99%

Latest Advances in Arbovirus Diagnostics

2023

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Arboviruses are a diverse family of vector-borne pathogens that include members of the Flaviviridae, Togaviridae, Phenuviridae, Peribunyaviridae, Reoviridae, Asfarviridae, Rhabdoviridae, Orthomyxoviridae and Poxviridae families. It is thought that new world arboviruses such as yellow fever virus emerged in the 16th century due to the slave trade from Africa to America. Severe disease-causing viruses in humans include Japanese encephalitis virus (JEV), yellow fever virus (YFV), dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), Crimean–Congo hemorrhagic fever virus (CCHFV), severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley fever virus (RVFV). Numerous methods have been developed to detect the presence of these pathogens in clinical samples, including enzyme-linked immunosorbent assays (ELISAs), lateral flow assays (LFAs) and reverse transcriptase–polymerase chain reaction (RT-PCR). Most of these assays are performed in centralized laboratories due to the need for specialized equipment, such as PCR thermal cyclers and dedicated infrastructure. More recently, molecular methods have been developed which can be performed at a constant temperature, termed isothermal amplification, negating the need for expensive thermal cycling equipment. In most cases, isothermal amplification can now be carried out in as little as 5–20 min. These methods can potentially be used as inexpensive point of care (POC) tests and in-field deployable applications, thus decentralizing the molecular diagnosis of arboviral disease. This review focuses on the latest developments in isothermal amplification technology and detection techniques that have been applied to arboviral diagnostics and highlights future applications of these new technologies.

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Section: Point Of Care Diagnosticsmentioning

confidence: 99%

Latest Advances in Arbovirus Diagnostics

2023

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“…También se han detectado citoquinas que se visualizan con LFA. 46 La implementación a gran escala de nuevas pruebas diagnósticas en aplicaciones clínicas y en salud pública todavía es limitada por una serie de factores: la falta de soluciones estandarizadas, los requisitos técnicos de los laboratorios, altos costos asociados con la implementación y el mantenimiento de estas tecnologías moleculares, la infraestructura de Internet y la computación en la nube. 47 Esperamos que abordar estas barreras mejore los resultados de los pacientes, pero aún se necesita una verdadera prueba POC para superar los desafíos restantes, como la preparación de muestras y las demandas de recursos humanos.…”

Section: Dispositivosunclassified

Diagnóstico no bacteriológico de la tuberculosis. Indicadores de la respuesta inmune. Parte II

González

Fruhwald²,

Duré

et al. 2023

Rev Am Med Resp

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“…In the past decade, the CRISPR-Cas system has been developed into multiple editing tools (Abdelrahman et al 2021 ; Singh et al 2022 ). Recently, Rahman et al reviewed the application of CRISPR-Cas-based molecular technologies in the diagnosis of several tropical diseases (Rahman et al 2022 ). According to effector modules, the CRISPR-Cas system is divided into type I and type II (Koonin and Makarova 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Four thermostatic steps: A novel CRISPR-Cas12-based system for the rapid at-home detection of respiratory pathogens

Fang

Liu

Cheng

et al. 2023

Appl Microbiol Biotechnol

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The outbreak of coronavirus disease 2019 (COVID-19) in 2019 has severely damaged the world's economy and public health and made people pay more attention to respiratory infectious diseases. However, traditional quantitative real-time polymerase chain reaction (qRT-PCR) nucleic acid detection kits require RNA extraction, reverse transcription, and amplification, as well as the support of large-scale equipment to enrich and purify nucleic acids and precise temperature control. Therefore, novel, fast, convenient, sensitive and specific detection methods are urgently being developed and moving to proof of concept test. In this study, we developed a new nucleic acid detection system, referred to as 4 Thermostatic steps (4TS), which innovatively allows all the detection processes to be completed in a constant temperature device, which performs extraction, amplification, cutting of targets, and detection within 40 min. The assay can specifically and sensitively detect five respiratory pathogens, namely SARS-CoV-2, Mycoplasma felis (MF), Chlamydia felis (CF), Feline calicivirus (FCV), and Feline herpes virus (FHV). In addition, a cost-effective and practical small-scale reaction device was designed and developed to maintain stable reaction conditions. The results of the detection of the five viruses show that the sensitivity of the system is greater than 94%, and specificity is 100%. The 4TS system does not require complex equipment, which makes it convenient and fast to operate, and allows immediate testing for suspected infectious agents at home or in small clinics. Therefore, the assay system has diagnostic value and significant potential for further reducing the cost of early screening of infectious diseases and expanding its application. Key points • The 4TS system enables the accurate and specific detection of nucleic acid of pathogens at 37 °C in four simple steps , and the whole process only takes 40 min. •A simple alkali solution can be used to extract nucleic acid. • A small portable device simple to operate is developed for home diagnosis and detection of respiratory pathogens. Supplementary Information The online version contains supplementary material available at 10.1007/s00253-023-12568-3.

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CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens

Cited by 3 publications

References 109 publications

Latest Advances in Arbovirus Diagnostics

Latest Advances in Arbovirus Diagnostics

Diagnóstico no bacteriológico de la tuberculosis. Indicadores de la respuesta inmune. Parte II

Four thermostatic steps: A novel CRISPR-Cas12-based system for the rapid at-home detection of respiratory pathogens

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