CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future

Zhang, Fangning; Neik, Ting Xiang; Thomas, William J. W.; Batley, Jacqueline

doi:10.3390/ijms24108623

Cited by 6 publications

(5 citation statements)

References 221 publications

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“…In expansion, Cas13 selectively knocks off cytoplasmic mRNA transcripts, enabling quicker downregulation of expression [34]. These finding suggests a promising opportunity to deploy this system for generating multigene silencing in polypoid plants [31,35].…”

Section: Cas13 Proteinmentioning

confidence: 93%

“…Cas13 proteins are displayed in at least 21 bacterial genomes and it consists of two distinct HEPN (higher eukaryote and prokaryote nucleotide binding) domains and a single protein effector [31] (Figure 3). CRISPR-Cas13 is the only known system among others to target single stranded RNA and owing to this ability, Cas13 has a potent application in plants to target RNA (coding and non-coding) and silence defense responses of RNA viruses [32].…”

Section: Cas13 Proteinmentioning

confidence: 99%

See 1 more Smart Citation

CRISPR/Cas Mediated Genome Engineering in Plants: Application and Future Prospective

Mishra,

Nayak,

Tuteja

et al. 2024

Preprint

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Genetic engineering has become an essential element in developing climate-resilient crops and environmental sustainable solutions to respond to the increasing need for global food security. Genome editing using CRISPR/Cas [Clustered regulatory interspaced short palindromic repeat (CRISPR)-associated protein (Cas)] technology is being applied to a variety of organisms, including plants. This technique has become popular because of its high specificity, effectiveness, and low production cost. Therefore, this technology has the potential to revolutionize agriculture and contribute to global food security. Over the past few years, increasing efforts have been seen in its application in developing higher-yielding, nutrition rich, disease resistant, stress-tolerant “crops” fruits and vegetables. Cas proteins such as Cas9, Cas12, Cas13, and Cas14, among others, have distinct architectures and have been used to create new genetic tools that improve features that are important for agriculture. The versatility of Cas has accelerated genomic analysis and facilitated the use of CRISPR/Cas to manipulate and alter nucleic acid sequences in cells of different organisms. This review provides the evolution of CRISPR technology exploring its mechanisms and contrasting it with traditional breeding and transgenic approaches to improve different stress tolerance. We have also discussed the CRISPR/Cas system and explored three Cas proteins that are currently known to exist: Cas12, Cas13, and Cas14 and their potential to generate foreign DNA free or non-transgenic crops that could be easily regulated for commercialization in most countries.

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Section: Cas13 Proteinmentioning

confidence: 93%

Section: Cas13 Proteinmentioning

confidence: 99%

CRISPR/Cas Mediated Genome Engineering in Plants: Application and Future Prospective

Mishra,

Nayak,

Tuteja

et al. 2024

Preprint

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“…New breeding techniques (NBTs) and in particular those based on genome editing (CRISPR/Cas9) encompasses an impressive and revolutionary set of molecular tools to enhance productivity by creating genetic variability for breeding purpose, disease-free and healthy planting genetic material, improvement in stress tolerance (Mote et al, 2022;Brower-Toland et al, 2023;Liu et al, 2023). The genome-editing approach can significantly accelerate the breeding times to select environmentally tolerant crop varieties (Zhang et al, 2023).…”

Section: Recent Updates In Omics For Heat Resiliencementioning

confidence: 99%

Plant responses to climate change, how global warming may impact on food security: a critical review

Janni,

Maestri,

Gullì

et al. 2024

Front. Plant Sci.

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Global agricultural production must double by 2050 to meet the demands of an increasing world human population but this challenge is further exacerbated by climate change. Environmental stress, heat, and drought are key drivers in food security and strongly impacts on crop productivity. Moreover, global warming is threatening the survival of many species including those which we rely on for food production, forcing migration of cultivation areas with further impoverishing of the environment and of the genetic variability of crop species with fall out effects on food security. This review considers the relationship of climatic changes and their bearing on sustainability of natural and agricultural ecosystems, as well as the role of omics-technologies, genomics, proteomics, metabolomics, phenomics and ionomics. The use of resource saving technologies such as precision agriculture and new fertilization technologies are discussed with a focus on their use in breeding plants with higher tolerance and adaptability and as mitigation tools for global warming and climate changes. Nevertheless, plants are exposed to multiple stresses. This study lays the basis for the proposition of a novel research paradigm which is referred to a holistic approach and that went beyond the exclusive concept of crop yield, but that included sustainability, socio-economic impacts of production, commercialization, and agroecosystem management.

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“…CRISPR-Cas13 has specificity for RNA sequences, whereas CRISPR-Cas9 targets DNA sequences for precision editing. The use of CRISPR technology in agricultural settings, particularly those of wheat, offers intriguing opportunities for crop development [42].…”

Section: Crispr Cas9; Cas13mentioning

confidence: 99%

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

Chachar,

Fan,

Chachar

et al. 2024

Agronomy

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Wheat (Triticum aestivum L.) is a fundamental crop essential for both human and animal consumption. Addressing the challenge of enhancing wheat yield involves sophisticated applications of molecular genetics and genomic techniques. This review synthesizes current research identifying and characterizing pivotal genes that impact traits such as grain size, number, and weight, critical factors influencing overall yield. Key genes including TaSPL17, ABP7, TaGNI, TaCKX6, TaGS5, TaDA1, WAPO1, TaRht1, TaTGW-7A, TaGW2, TaGS5-3A, TaSus2-2A, TaSus2-2B, TaSus1-7A, and TaSus1-7B are examined for their roles in these traits. The review also explores genes responsive to environmental changes, which are increasingly significant under current climate variability. Multi-trait regulatory genes and quantitative trait loci (QTLs) that contribute to these traits are discussed, highlighting their dual influences on grain size and yield. Furthermore, the paper underscores the utility of emerging technologies such as CRISPR/Cas9, Case13, and multi-omics approaches. These innovations are instrumental for future discoveries and are poised to revolutionize wheat breeding by enabling precise genetic enhancements. Facing unprecedented challenges from climate change, the identification and utilization of these candidates is crucial. This review aims to be a comprehensive resource for researchers, providing an integrative understanding of complex traits in wheat and proposing new avenues for research and crop improvement strategies.

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CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future

Cited by 6 publications

References 221 publications

CRISPR/Cas Mediated Genome Engineering in Plants: Application and Future Prospective

CRISPR/Cas Mediated Genome Engineering in Plants: Application and Future Prospective

Plant responses to climate change, how global warming may impact on food security: a critical review

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

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