CRISPR-based genetic interaction maps inform therapeutic strategies in cancer

Ramkumar, Poornima; Kampmann, Martin

doi:10.21037/tcr.2018.01.02

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…The findings revealed that the deletion of the insulin-like growth factor 1 receptor (IGF1R) promotes MET amplification and reduces the mesenchymal level, leading to acquired resistance to erlotinib in these cells. These studies and numerous other studies (Ramkumar and Kampmann, 2018;Jalaleddine et al, 2019;Christodoulou et al, 2021;Akram et al, 2022;Przybyla and Gilbert, 2022) underscore the invaluable role of 2D cellular models in advancing our understanding of cancer biology and facilitating drug development.…”

Section: In Vitro Cancer Modeling Using Crispr/cas9mentioning

confidence: 79%

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

Ravichandran,

Maddalo

2023

Front. Genet.

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The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery.

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Section: In Vitro Cancer Modeling Using Crispr/cas9mentioning

confidence: 79%

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

Ravichandran,

Maddalo

2023

Front. Genet.

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“…In addition to prob ing single gene perturbations, CRISPR can be used to identify essential genetic interactions 232,233 . Targeting multiple genes simultaneously in a single cell can reveal synthetic lethal interactions or potential drug combina tions, or define cancer dependencies that are buffered by related, redundant family members (for example, MEK1 and MEK2) 234 .…”

Section: Identifying Gene Interactionsmentioning

confidence: 99%

CRISPR in cancer biology and therapy

et al. 2022

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“…With the help of these libraries, it became possible to conduct genome-wide loss-of-function studies. This approach has been used extensively to map genetic dependencies of cancer lines [72], for systematic discovery of genetic interactions [73] and for elucidation of resistance factors to particular therapies [74].…”

Section: Genome-wide Screeningmentioning

confidence: 99%

Applications of CRISPR-Cas Technologies to Proteomics

Dolgalev

Poverennaya

2021

Genes

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CRISPR-Cas-based genome editing is a revolutionary approach that has provided an unprecedented investigational power for the life sciences. Rapid and efficient, CRISPR-Cas technologies facilitate the generation of complex biological models and at the same time provide the necessary methods required to study these models in depth. The field of proteomics has already significantly benefited from leveraging the power of CRISPR-Cas technologies, however, many potential applications of these technologies in the context of proteomics remain unexplored. In this review, we intend to provide an introduction to the CRISPR-Cas technologies and demonstrate how they can be applied to solving proteome-centric questions. To achieve this goal, we begin with the description of the modern suite of CRISPR-Cas-based tools, focusing on the more mature CRISPR-Cas9 system. In the second part of this review, we highlight both established and potential applications of the CRISPR-Cas technologies to proteomics.

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CRISPR-based genetic interaction maps inform therapeutic strategies in cancer

Cited by 8 publications

References 25 publications

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

CRISPR in cancer biology and therapy

Applications of CRISPR-Cas Technologies to Proteomics

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