CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus

Koutmani, Yassemi; Gampierakis, Ioannis A.; Polissidis, Alexia; Ximerakis, Methodios; Koutsoudaki, Paraskevi N.; Polyzos, A.; Agrogiannis, George; Karaliota, Sevasti; Thomaidou, Dimitra; Rubin, Lee L.; Politis, Panagiotis K.; Karalis, Katia

doi:10.1016/j.celrep.2019.09.037

Cited by 17 publications

(24 citation statements)

References 59 publications

(69 reference statements)

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“…The alternative to the single factors presented above is suggested by several protocols that propose the use of chemical compound "cocktails". There are attempts to use mixes containing factors stimulating neuronal differentiation, such as forskolin, insulin, hydrocortisone, and valproic acid in the media [25,44], ongoing both with the maintenance of the cells' high proliferation rate. In this study, we analyzed the impact of one of them that was not previously used in ASCs' differentiation, commercially available supplement N21.…”

Section: Introductionmentioning

confidence: 99%

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Figiel-Dabrowska

Radoszkiewicz

Rybkowska

et al. 2021

Cells

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Currently, the number of stem-cell based experimental therapies in neurological injuries and neurodegenerative disorders has been massively increasing. Despite the fact that we still have not obtained strong evidence of mesenchymal stem/stromal cells’ neurogenic effectiveness in vivo, research may need to focus on more appropriate sources that result in more therapeutically promising cell populations. In this study, we used dedifferentiated fat cells (DFAT) that are proven to demonstrate more pluripotent abilities in comparison with standard adipose stromal cells (ASCs). We used the ceiling culture method to establish DFAT cells and to optimize culture conditions with the use of a physioxic environment (5% O2). We also performed neural differentiation tests and assessed the neurogenic and neuroprotective capability of both DFAT cells and ASCs. Our results show that DFAT cells may have a better ability to differentiate into oligodendrocytes, astrocytes, and neuron-like cells, both in culture supplemented with N21 and in co-culture with oxygen–glucose-deprived (OGD) hippocampal organotypic slice culture (OHC) in comparison with ASCs. Results also show that DFAT cells have a different secretory profile than ASCs after contact with injured tissue. In conclusion, DFAT cells constitute a distinct subpopulation and may be an alternative source in cell therapy for the treatment of nervous system disorders.

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Section: Introductionmentioning

confidence: 99%

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Figiel-Dabrowska

Radoszkiewicz

Rybkowska

et al. 2021

Cells

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“…Data represent the mean ± SEM. *P ≤ .05 quantification of SOX2-and doublecortin-positive cells, series of every sixth brain section were stained and positive cells were counted throughout the rostro-caudal extent of the granule cell layer(Koutmani et al, 2019).…”

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confidence: 99%

Detrimental effects of adolescent escalating low‐dose Δ⁹‐tetrahydrocannabinol leads to a specific bio‐behavioural profile in adult male rats

Poulia

Delis

Brakatselos

et al. 2021

British J Pharmacology

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Background and Purpose: Adolescent cannabis use is associated with adult psychopathology. When Δ 9-tetrahydrocannabinol (THC), mainly in high doses, is administered to adolescence rats there are also long lasting effects in adults. This study aims to determine the specific adult bio-behavioural profile after adolescent low-dose THC, which better mirrors adolescent recreational cannabis use. Experimental Approach: Adolescent male Sprague-Dawley rats were treated with escalating low-dose of THC. In adulthood, they were evaluated for their spontaneous locomotion, sensorimotor gating, higher order and spatial cognitive functions. Dopaminergic activity and cannabinoid receptor expression were measured in distinct brain regions. Hippocampal neurogenic activity of neural stem cells was determined and protein levels of neuroplasticity-related biomarkers were quantified. Adolescent low-dose THC exposure increased spontaneous open-field activity, without affecting prepulse inhibition and attentional set-shifting performance. Region-specific dopaminergic alterations and CB 1 receptor up-regulation in the prefrontal cortex were observed. Impaired spatial memory, as assessed with the object location task and Morris water maze test, was associated with significantly decreased proliferative activity (SOX2-positive cells), neurogenic potential (decreased doublecortin-positive cells) in the adult hippocampus and defective neuroplasticity, including reduced BDNF expression in the hippocampus and prefrontal cortex. Key Results: Our findings reveal the adverse impact of adolescent low-dose THC on the psychomotor profile, dopaminergic neurotransmission, compensatory cannabinoid receptor response, cognition-related neurobiological and behavioural functions. Conclusion and Implications: Our adolescent low-dose THC animal model does not induce tangible psychotic-like effects, such as those reported in high-dose THC studies, but it impairs cognitive functions and points to hippocampal vulnerability and disrupted neurogenesis.

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“…In the newborn rat, the PVN and CA1 of the hippocampus contain GR mRNA expression ( Pryce, 2008 ). The CRH hippocampal system regulates neurogenesis in the hippocampus which is involved in spatial learning and memory ( Koutmani et al, 2019 ). Elevated levels of glucocorticoids have also been shown to impair working and reference memory ( Stylianakis et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

The Long-Term Effects of Neonatal Inflammatory Pain on Cognitive Function and Stress Hormones Depend on the Heterogeneity of the Adolescent Period of Development in Male and Female Rats

Butkevich

Mikhailenko

Vershinina

et al. 2021

Front. Behav. Neurosci.

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Exposure to stress at an early age programs the HPA axis which can lead to cognitive deficits in adults. However, it is not known whether these deficits emerge in adulthood or are expressed earlier in life. The aims of the study were to investigate (1) the immediate effects of early injury-induced stress in one-day-old (P1) and repeated stress on at P1 and P2 rat pups on plasma corticosterone levels; and (2) examine the subsequent long-term effects of this early stress on spatial learning and memory, and stress reactivity in early P26-34 and late P45-53 adolescent male and female rats. Intra-plantar injection of formalin induced prolonged and elevated levels of corticosterone in pups and impaired spatial learning and short- and long-term memory in late adolescent males and long-term memory in early adolescent females. There were sex differences in late adolescence in both learning and short-term memory. Performance on the long-term memory task was better than that on the short-term memory task for all early adolescent male and female control and stressed animals. Short-term memory was better in the late age control rats of both sexes and for formalin treated females as compared with the early age rats. These results are consistent with an impaired function of structures involved in memory (the hippocampus, amygdala, prefrontal cortex) after newborn pain. However, activation of the HPA axis by neonatal pain did not directly correlate with spatial learning and memory outcomes and the consequences of neonatal pain remain are likely multi-determined.

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CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus

Cited by 17 publications

References 59 publications

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Detrimental effects of adolescent escalating low‐dose Δ⁹‐tetrahydrocannabinol leads to a specific bio‐behavioural profile in adult male rats

The Long-Term Effects of Neonatal Inflammatory Pain on Cognitive Function and Stress Hormones Depend on the Heterogeneity of the Adolescent Period of Development in Male and Female Rats

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CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus

Cited by 17 publications

References 59 publications

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue

Detrimental effects of adolescent escalating low‐dose Δ9‐tetrahydrocannabinol leads to a specific bio‐behavioural profile in adult male rats

The Long-Term Effects of Neonatal Inflammatory Pain on Cognitive Function and Stress Hormones Depend on the Heterogeneity of the Adolescent Period of Development in Male and Female Rats

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Detrimental effects of adolescent escalating low‐dose Δ⁹‐tetrahydrocannabinol leads to a specific bio‐behavioural profile in adult male rats