Crecimiento de juveniles de congrio colorado Genypterus chilensis en condiciones de cultivo

Vega, Rolando; Estrada, Juan Manuel; Ramírez, Diego García; Flores, Camila; Zamorano, José; Encina, Francisco; Mardones, Alfonso; Valdebenito, Iván; Dantagnan, Patricio

doi:10.3856/vol43-issue2-fulltext-11

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…The red cusk-eel ( Genypterus chilensis ) is a new economically important marine species for the Chilean aquaculture industry [ 22 ], but low handling-stress tolerance results in high mortality rates during juvenile stages [ 23 ]. Handling-stress facilitates red cusk-eel skeletal muscle atrophy through the coordinated expression of components of the ubiquitin-proteasome and autophagy-lysosome systems, revealing particularities in the compensatory response to stress [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis

et al. 2017

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Teleosts exhibit a broad divergence in their adaptive response to stress, depending on the magnitude, duration, and frequency of stressors and the species receiving the stimulus. We have previously reported that the red cusk-eel (Genypterus chilensis), an important marine farmed fish, shows a physiological response to stress that results in increased skeletal muscle atrophy mediated by over-expression of components of the ubiquitin proteasome and autophagy-lysosomal systems. To better understand the systemic effects of stress on the red cusk-eel metabolism, the present study assessed the transcriptomic hepatic response to repetitive handling-stress. Using high-throughput RNA-seq, 259 up-regulated transcripts were found, mostly associated with angiogenesis, gluconeogenesis, and triacylglyceride catabolism. Conversely, 293 transcripts were down-regulated, associated to cholesterol biosynthesis, PPARα signaling, fatty acid biosynthesis, and glycolysis. This gene signature was concordant with hepatic metabolite levels and hepatic oxidative damage. Moreover, the increased plasmatic levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase) and AP (alkaline phosphatase), as well as liver histology suggest stress-induced liver steatosis. This study offers an integrative molecular and biochemical analysis of the hepatic response to handling-stress, and reveals unknown aspects of lipid metabolism in a non-model teleost.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis

et al. 2017

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“…Regarding Genypterus chilensis, it is a coastal bathydemersal endemic species, and it is distributed from Africa (18 • 25 S) until Los Chonos Archipelago (47 • 70 S) at depths ranging from 20 m to 150 m [5]. It has a carnivorous diet, being their common preys, crustaceans of small size, and fish [6,7]. The fishery of Genypterus chilensis is mainly limited to the non-industrial activity [4], with annual captures between 400 to 900 tons in the last years [1].…”

Section: Introductionmentioning

confidence: 99%

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

et al. 2022

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Chile has promoted the diversification of aquaculture and red cusk-eel (Genypterus chilensis) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element that may play an important role in the nutrition and defense of cultured animals for meat production. This study compares the microbiota composition of the intestinal contents of wild and aquaculture fish to explore the microbial communities present and their potential contribution to the host. DNA was extracted from the intestinal content samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the Ion Torrent platform. After the examination of the sequences, strong differences were found in the composition at the level of phylum, being Firmicutes and Tenericutes the most abundant in aquaculture and wild condition, respectively. At the genus level, the Vagococcus (54%) and Mycoplasma (97%) were the most prevalent in the microbial community of aquaculture and wild condition, respectively. The evaluation of predicted metabolic pathways in these metagenomes showed that in wild condition there is an important presence of lipid metabolism belonging to the unsaturated fatty acid synthesis. In the aquaculture condition, the metabolism of terpenoids and polyketides were relevant. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of red cusk-eel (Genypterus chilensis) of wild and aquaculture origin using high-throughput sequencing.

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“…In this work, viscera and liver from farmed salmon will be assessed as alternative sources of EPA and DHA together with viscera from wild red cusk‐eel ( Genipterus chilensis ) and yellowtail kingfish ( Seriola lalandi ), called “congrio colorado” and “palometa” in Chile, respectively. Although production of red cusk‐eel and yellowtail kingfish in Chile is much lower than that of salmon, with 636 and 372 Mt reported in 2014, respectively (SERNAPESCA), both species are commercially interesting: red cusk‐eel is a fish with a high demand in the Chilean market due to its exceptional flesh quality . Yellowtail kingfish is popular because of its firm, white, and slightly oily flesh, and it is widely used for sashimi preparation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of fatty acid profiles of dried and raw by‐products from cultured and wild fishes

Rincón‐Cervera

Villarreal‐Rubio

Valenzuela

et al. 2017

Euro J Lipid Sci & Tech

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Fish by‐products may become alternative sources of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, due to the high enzymatic activity in these biological tissues, special care must be taken to prevent lipid oxidation and hydrolysis. In this work, several by‐products from Chilean fishes (farmed salmon and wild red cusk‐eel and yellowtail kingfish) were dried at 105°C for 3 h to remove water and inactivate enzymes. The effect of temperature on EPA and DHA levels was assessed by comparing fatty acid profiles of raw and dried by‐products. Drying at 105°C for 3 h was considered an adequate process to obtain dried powders from fish by‐products with appreciable amounts of EPA and DHA, even though EPA and DHA values showed a certain decrease after drying. Several methodologies involving food‐grade solvents were checked to evaluate their suitability for lipid extraction from dried by‐products, being Soxhlet extraction with n‐hexane identified as the most suitable process in terms of extraction yield and EPA/DHA values. Cholesterol amount was also studied, being the highest and lowest amounts found in liver and viscera from farmed salmon, respectively. Practical applications: In fish processing plants, raw by‐products are collected after fish evisceration, and they can be transported to oil extraction facilities, although their lipids may be easily degraded unless special precautions are taken to preserve such biomass. Raw fish by‐products must be subjected to water removal and enzyme inactivation to prevent lipid degradation and hydrolysis, and it is desirable that such actions are carried out in the processing plants themselves to ensure the maximum oil quality. Drying at 105°C for a short time (3 h) was assayed in this work because of its simplicity, low cost, scalability, and feasibility to be installed in fish processing plants. Soxhlet procedure with n‐hexane is effective to extract lipids containing EPA and DHA from dried by‐products for nutritional or nutraceutical purposes. Because of water removal, lipid extraction efficiency from dried by‐products is enhanced and less solvent is needed, which is economically and environmentally desirable. Raw by‐products from cultured and wild fishes are dried at 105°C for 3 h and fatty acid profiles are compared between raw and dried by‐products to assess the potential decreasing effect of temperature on the levels of polyunsaturated fatty acids, especially EPA and DHA. Different lipid extractions using solvents are also compared to check their influence on fatty acid profiles. Additionally, cholesterol amount is quantified in the lipid fraction extracted from dried fish by‐products.

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Crecimiento de juveniles de congrio colorado Genypterus chilensis en condiciones de cultivo

Cited by 10 publications

References 17 publications

Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis

Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

Comparison of fatty acid profiles of dried and raw by‐products from cultured and wild fishes

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