Creation of rabbit bone and soft tissue tumor using cultured VX2 cells

Handal, John A.; Schulz, Jacob F.; Florez, Gerson B.; Kwok, Simon C. M.; Khurana, Jasvir S.; Samuel, Solomon Praveen

doi:10.1016/j.jss.2012.02.061

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…VX2 tissue was obtained from VX2 tumor bearing NZW rabbits. The tumor tissue was minced and propagated in DMEM:F12 (1:1) media (7). Outgrowing cells were continuously sub cultured for more than 150 passages (Fig.…”

Section: Discussionmentioning

confidence: 99%

Etablierung und Charakterisierung einer Kaninchen VX2 Karzinom Zelllinie als Modellsystem für Papillomavirus assoziierte Kopf-Halstumoren

Ali

Ambreen

Pinnapireddy³

et al. 2019

Abstract- Und Posterband – 90. Jahresversammlung Der Deutschen Gesellschaft Für HNO-Heilkunde, Kopf- Und Hals-Chirurgie e.V., B

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Figure 3. Characterization of the VX2 cell line by flow cytometry and Western blot analysis. (A) Two distinct populations of VX2 cells are observed during FACS analysis. One population was designated as small and the other was termed large based on their different cell sizes and granularity. (B) After sorting each of the two populations into a single tissue culture flask both subpopulations reappeared during further culturing pointing them to represent two phenotypes of the same cell line. (C) Western Blot analysis of unsorted VX2 cells as well as the small and the large populations demonstrated expression of the epidermal growth factor receptor (EGFR) with pronounced activity of this signaling pathway (Erk1/2-phospho) in the large population. EGFR: epidermal growth factor receptor, TrkB: tropomyosin-related kinase B, Erk1/2-phospho: extracellular signal regulated kinase 1 and 2 (active form), CXCR4: C-X-C chemokine receptor type 4 (=CD184), PCNA: proliferating cell nuclear antigen, Pin1: peptidyl-prolyl cis-trans isomerase 1.

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Section: Discussionmentioning

confidence: 99%

Etablierung und Charakterisierung einer Kaninchen VX2 Karzinom Zelllinie als Modellsystem für Papillomavirus assoziierte Kopf-Halstumoren

Ali

Ambreen

Pinnapireddy³

et al. 2019

Abstract- Und Posterband – 90. Jahresversammlung Der Deutschen Gesellschaft Für HNO-Heilkunde, Kopf- Und Hals-Chirurgie e.V., B

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“…Rabbit VX2 cancer models of the liver, kidney, lung and bone have been studied maturely, and have been widely applied for use in tumor imaging, physiological and pathological studies, as well as in the efficacy evaluation of interventional treatment (14). However, VX2 brain tumor animal models have been rarely used in domestic and foreign studies, and the tumor implantation method used is primarily the intracerebral injection of tumor cell suspension (15,16). At the start of the present study, a number of other methods had been attempted; however, various shortcomings were observed.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging-navigated argon-helium cryoablation therapy against a rabbit VX2 brain tumor

Wang

Kan

Sun

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the feasibility of interventional magnetic resonance imaging (MRI)-guided and monitored argon-helium cryoablation for the treatment of brain tumors in rabbits. In addition, the present study evaluated the associations between imaging and pathology, the therapeutic effects and the effects on the surrounding normal tissues. A total of 14 rabbits were equally divided into groups C and D. Under general anesthesia, the skull was drilled and tumor blocks were implanted. Subsequently, a New Zealand rabbit VX2 brain tumor model was successfully established. Rabbits in group C were treated with argon-helium cryoablation and those in group D did not undergo any treatment (control). Regular postoperative MRI scanning was performed to observe the changes in tumor size, and the survival times of the rabbits in groups C and D were recorded. The extent of necrosis in the brain tumor exhibited a significant correlation with the freezing time of cryoablation, and the necrotic region was shown to be the same size as the ice ball. The survival times of the rabbits in the treatment group (group C) were significantly prolonged. Therefore, the observations of the present study demonstrated that the VX2 brain tumor model, produced using an improved tumor block implantation method, was stable and suitable for MRI observation and interventional study. In addition, argon-helium cryoablation was shown to be a safe and feasible therapeutic method for the treatment of brain tumors, and was demonstrated to significantly increase the survival times of the brain tumor-bearing rabbits. IntroductionPrimary brain tumors have a high incidence rate, and tumor growth is invasive with an obscure boundary (1). Although brain tumors can be resected through various treatment methods, including surgery, radiotherapy and chemotherapy, disease progression is unable to be effectively controlled. In addition, the prognosis of patients with a brain tumor is usually poor, with a short survival time and severe sequelae (2).Magnetic resonance imaging (MRI)-navigated argonhelium cryoablation is a new advanced technology. MRI is known to have good tissue resolution and a multiplanar imaging capability. In particular, the temperature-sensitive characteristics of MRI, which enable the clear and real-time display of the size and range of the ice ball (which destroys the tumor cells), has resulted in this imaging technique becoming the focus in clinical cryotherapy against multiple-system tumors, with satisfactory results previously achieved (3-7). Although MRI has an unparalleled advantage in nervous system imaging compared with other methods (8), there are a limited number of domestic and foreign studies investigating MRI-navigated cryotherapy for the treatment of brain tumors.In the present study, a rabbit VX2 brain tumor model was established that was suitable for MRI imaging and interventional therapy. The model was used for the preliminary study of MRI-navigated argon-helium cryoablation therapy against t...

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“…tissue was obtained from VX2 tumor bearing NZW rabbits. The tumor tissue was minced and propagated in DMEM:F12 (1:1) media (7). Outgrowing cells were continuously sub cultured for more than 150 passages (Fig.…”

Section: Materials and Methods Vx2mentioning

confidence: 99%

Establishment and characterization of a VX2 carcinoma derived rabbit cell line as a model system for the study of papilloma virus associated head and neck cancer

Ali

Ambreen

Pinnapireddy³

et al. 2019

Abstract- Und Posterband – 90. Jahresversammlung Der Deutschen Gesellschaft Für HNO-Heilkunde, Kopf- Und Hals-Chirurgie e.V., B

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A B C Figure 3. Characterization of the VX2 cell line by flow cytometry and Western blot analysis. (A) Two distinct populations of VX2 cells are observed during FACS analysis. One population was designated as small and the other was termed large based on their different cell sizes and granularity. (B) After sorting each of the two populations into a single tissue culture flask both subpopulations reappeared during further culturing pointing them to represent two phenotypes of the same cell line. (C) Western Blot analysis of unsorted VX2 cells as well as the small and the large populations demonstrated expression of the epidermal growth factor receptor (EGFR) with pronounced activity of this signaling pathway (Erk1/2-phospho) in the large population. EGFR: epidermal growth factor receptor, TrkB: tropomyosin-related kinase B, Erk1/2-phospho: extracellular signal regulated kinase 1 and 2 (active form), CXCR4: C-X-C chemokine receptor type 4 (=CD184), PCNA: proliferating cell nuclear antigen, Pin1: peptidyl-prolyl cis-trans isomerase 1.

show abstract

Creation of rabbit bone and soft tissue tumor using cultured VX2 cells

Cited by 12 publications

References 14 publications

Etablierung und Charakterisierung einer Kaninchen VX2 Karzinom Zelllinie als Modellsystem für Papillomavirus assoziierte Kopf-Halstumoren

Etablierung und Charakterisierung einer Kaninchen VX2 Karzinom Zelllinie als Modellsystem für Papillomavirus assoziierte Kopf-Halstumoren

Magnetic resonance imaging-navigated argon-helium cryoablation therapy against a rabbit VX2 brain tumor

Establishment and characterization of a VX2 carcinoma derived rabbit cell line as a model system for the study of papilloma virus associated head and neck cancer

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