Creation of myocardial tubes using cardiomyocyte sheets and an in vitro cell sheet-wrapping device

Kubo, Hirotsugu; Shimizu, Tatsuya; Yamato, Masayuki; Fujimoto, Tetsuo; Okano, Teruo

doi:10.1016/j.biomaterials.2007.04.016

Cited by 105 publications

(64 citation statements)

References 30 publications

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“…Combining stem cell technology with bioengineering methods, such as 3D artificial cardiac constructs comprising cardiomyocytes, endothelial cells and fibroblasts, has shown promise in addressing this issue. [96][97][98] A recent article from the Ruiz-Lozano group showed that reconstitution of follistatinlike 1, a regenerative factor secreted by the healthy epicardium, using a bioengineered epicardial patch improved cardiac function in mouse and swine models of MI. 99 As for these cellfree approaches, the safety of the factors-be it recombinant proteins for promoting cardiomyocyte growth, miRNAs for promoting dedifferentiation, or viral vectors for cellular reprogramming-has to be carefully monitored in vivo.…”

Section: Future Prospectivesmentioning

confidence: 99%

Mending a Faltering Heart

Belmonte

2016

Circulation Research

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More people die every year from ischemic heart disease than any other disease. Because the human heart lacks sufficient ability to replenish the damaged cardiac muscles, extensive research has been devoted toward understanding the homeostatic and regenerative potential of the heart and to develop regenerative therapies for heart disease. Here, we discuss recent advances in the understanding of mechanisms governing heart growth during homeostasis or injury, including those from observational studies in humans and experimental research in animal models of cardiac regeneration. We also discuss how progress in stem cell biology and cellular reprogramming has enabled exciting new strategies for cardiac regeneration. (Circ Res. 2016;118:344-351.

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Section: Future Prospectivesmentioning

confidence: 99%

Mending a Faltering Heart

Belmonte

2016

Circulation Research

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“…If sufficient neovascularization occurs, a new layer of cell sheets can be applied on top of the previous one, after which neovascularization of the new layer of cell sheets takes place. Through this kind of polysurgery, which applies the use of repeated transplantations after neovascularization, thick muscle tissue with connectable vessels may be produced (Shimizu et al, 2006a(Shimizu et al, , 2006bYang et al, 2007;Kubo et al, 2007). This technique has not yet been used for engineering skeletal muscle, and the current method is not practical in a human situation.…”

Section: Vascularizationmentioning

confidence: 99%

Current opportunities and challenges in skeletal muscle tissue engineering

Koning¹,

Harmsen²,

Luyn³

et al. 2009

J Tissue Eng Regen Med

144

108

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The purpose of this article is to give a concise review of the current state of the art in tissue engineering (TE) of skeletal muscle and the opportunities and challenges for future clinical applicability. The endogenous progenitor cells of skeletal muscle, i.e. satellite cells, show a high proneness to muscular differentiation, in particular exhibiting the same characteristics and function as its donor muscle. This suggests that it is important to use an appropriate progenitor cell, especially in TE facial muscles, which have a exceptional anatomical and fibre composition compared to other skeletal muscle. Muscle TE requires an instructive scaffold for structural support and to regulate the proliferation and differentiation of muscle progenitor cells. Current literature suggests that optimal scaffolding could comprise of a fibrin gel and cultured monolayers of muscle satellite cells obtained through the cell sheet technique. Tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen, carbon dioxide, nutrients and waste products. Finally, functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural cells. To reach this, it seems important to apply optimal electrical, chemotropic and mechanical stimulation during engineering and discover other factors that influence its formation. Thus, in addition to approaches for myogenesis, we discuss the current status of strategies for angiogenesis and neurogenesis of TE muscle constructs and the significance for future clinical use.

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“…We are now trying to simulate this in vivo situation in vitro. We also succeeded to create tubular pulsatile tissues by wrapping cardiomyocyte sheets around a blood vessel or tubular scaffold (Kubo et al, 2007;. We confirmed that the beating of thus prepared myocardial tube can contribute to blood pressure independently of intrinsic heartbeat.…”

Section: Cell-sheet Engineeringmentioning

confidence: 52%