Creation of blast disease-resistant rice varieties with modern DNA-markers

Dubina, Elena; Mukhina, Zhanna; Kharitonov, E. M.; Shilovskiy, V N; Kharchenko, E. S.; Esaulova, Lyubov; Korkina, N. N.; Maximenko, E. P.; Nikitina, I. B.

doi:10.1134/s1022795415060058

Cited by 10 publications

(12 citation statements)

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“…The use of specialized DNA markers associated with disease resistance provides a clear control of the inheritance of the target locus. This helps to reduce the time and material costs in modeling and creating a promising genetic plasma [28]. The result of this work is the creation of a new variety of rice Utyos obtained by individual selection from F2BC4 Flagman / IR-36, with repeated selection and verification of offspring.…”

Section: Research Resultsmentioning

confidence: 99%

Innovative trends in breeding disease resistant rice varieties

et al. 2021

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Rice has become widespread in world agriculture due to its high adaptation to growing conditions and the selection of many different types of varieties. The creation of new varieties of rice in Russia has been carried out using the methods of complex multistage hybridization and marker selection with close cooperation of breeders and biotechnologists. The result of this work is a mid-season variety Utyos that combines high yields with resistance to blast, the main fungal disease of rice that affects leaves, stems and panicles of the plant. The late-ripening rice variety IR-36, which has complex resistance to fungal and bacterial diseases and viruses, was used as a donor. Variety Utyos was created by selecting an elite plant from the F2 BC4 Flagship / IR-36 population, with repeated selection and verification by offspring. Starting from the first backcrossing, marker control was carried out for the presence of transferred donor alleles in the hybrid offspring. In parallel, phytopathological control was carried out against an infectious background. In 2019, in environmental tests, Utyos formed the maximum grain yield of 11.95 t / ha with winter wheat as the predecessor. At the same time, the variety showed high resistance to blast.

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Section: Research Resultsmentioning

confidence: 99%

Innovative trends in breeding disease resistant rice varieties

et al. 2021

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“…Plants were selected that were closest in morphotype to the recipient parental form and had donor genes for resistance to the pathogen Pyricularia oryzae Cav. in their genotype in a homozygous state [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

Rice Breeding in Russia Using Genetic Markers

Dubina

Kostylev

Ruban

et al. 2020

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The article concentrates on studying tolerance to soil salinization, water flooding, and blast in Russian and Asian rice varieties, as well as hybrids of the second and third generations from their crossing in order to obtain sustainable paddy crops based on domestic varieties using DNA markers. Samples IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, and NSIC Rc 106 were used as donors of the SalTol tolerance gene. Varieties with the Sub1A locus were used as donors of the flood resistance gene: Br-11, CR-1009, Inbara-3, TDK-1, and Khan Dan. The lines C101-A-51 (Pi-2), C101-Lac (Pi-1, Pi-33), IR-58 (Pi-ta), and Moroberekan (Pi-b) were used to transfer blast resistance genes. Hybridization of the stress-sensitive domestic varieties Novator, Flagman, Virazh, and Boyarin with donor lines of the genes of interest was carried out. As a result of the studies carried out using molecular marking based on PCR in combination with traditional breeding, early-maturing rice lines with genes for resistance to salinity (SalTol) and flooding (Sub1A), suitable for cultivation in southern Russia, were obtained. Introgression and pyramiding of the blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b into the genotypes of domestic rice varieties were carried out. DNA marker analysis revealed disease-resistant rice samples carrying 5 target genes in a homozygous state. The created rice varieties that carry the genes for blast resistance (Pentagen, Magnate, Pirouette, Argamac, Kapitan, and Lenaris) were submitted for state variety testing. The introduction of such varieties into production will allow us to avoid epiphytotic development of the disease, preserving the biological productivity of rice and obtaining environmentally friendly agricultural products.

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“…Для успешной селекции на устойчивость к данному заболеванию у селекционера в первую очередь должны быть информация об эффективных генах устойчивости для местной зоны возделывания культуры риса, а также сведения о биоразнообразии P. oryzae (Dubina et al, 2015).…”

Section: Development Of Blast-resistant Rice Varieties Based On Appliunclassified