Creation of Bifurcation-Type Elastase-Induced Aneurysms in Rabbits

Ding, Yong Hong; Kadirvel, Ramanathan; Dai, Daying; Kallmes, David F.

doi:10.3174/ajnr.a2666

Cited by 9 publications

(7 citation statements)

References 13 publications

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“…A bifurcation type aneurysm has been induced in rabbit carotid arteries and can provide additional insight regarding the contribution of flow diversion to aneurysm formation. 25,35 Size restricts endovascular research in mice, but the combination of PPE modifications presented here enables the mimicking of different types of aortic aneurysm morphologies for imaging and molecular studies in future translational research (Figs. 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Four Surgical Modifications to the Classic Elastase Perfusion Aneurysm Model Enable Haemodynamic Alterations and Extended Elastase Perfusion

Busch

Chernogubova

Jin

et al. 2018

European Journal of Vascular and Endovascular Surgery

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Section: Discussionmentioning

confidence: 99%

Four Surgical Modifications to the Classic Elastase Perfusion Aneurysm Model Enable Haemodynamic Alterations and Extended Elastase Perfusion

Busch

Chernogubova

Jin

et al. 2018

European Journal of Vascular and Endovascular Surgery

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“…There are reports about generation of elastase-induced aneurysms in rabbits [18][19][20][21][22][23][24][25][26][27][28][29][30], but this represents the first attempt to explore a simple, effective, and reproducible method for creating elastase-induced aneurysms by comparing three different methods in rabbits. We have the lowest mortality (5.6% in Group B) and the similar success rate in creating aneurysms in the three methods, so Group B is the best recommended elastase-induced aneurysm method.…”

Section: Discussionmentioning

confidence: 99%

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

Lyu

Luo

Zhang

et al. 2020

BioMed Research International

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Objective. Elastase-induced aneurysms in rabbits have been proposed as a preclinical tool for device development, but there is still much deficiency in those aneurismal models. So we need to explore the efficient and convenient animal models for the investigation of intracranial aneurysms. Then, we compared and analyzed three methods of elastase-induced carotid artery aneurysms in rabbits and aimed to find a simple, effective, and reproducible method for creating elastase-induced aneurysms. Methods. 42 standard feeding male adult Japanese white rabbits (3.05±0.65 kg) were randomly divided into 3 groups and treated with elastase ablation to create common carotid artery (RCCA) aneurysm models: Group A (root-RCCA medication group, n=12), Group B (mid-RCCA medication group, n=18), and Group C (ligated RCCA+medication group, n=12). For Group A, the origin of the RCCA was blocked by two temporary aneurysm clips, and the resulting 2 cm cavity was infused with elastase for 20 min, then the clip was removed and the RCCA was not ligated. For Group B, the middle part of RCCA was treated the same way as Group A and the RCCA was not ligated. For Group C, the middle part of RCCA was treated as Group B, but the distal RCCA was ligated. After the aneurysm models were created for 3 weeks, prior to sacrificing the animals, color Doppler ultrasound and angiography were performed for blood flow measurements inside the aneurysms. Histological analysis (such as SMA-α, CD31, CD34, CD68, collagen IV, and Ki67) and the other relevant indexes were compared between the ideal model’s aneurysmal tissues and the human intracranial aneurysm’s tissues to confirm whether we have successfully established elastase-induced aneurysm models. Results. Compared with human intracranial aneurysm specimens by the color Doppler ultrasound, angiography, and changes in the inner diameter of arteries, all three methods have successfully established the elastase-induced aneurysm models. Histology showed that biological responses were similar to both human cerebral aneurysms and previously published elastase-induced rabbit aneurysm models. Group A and Group B had the same morphology, but Group A had a higher mortality rate than Group B. Group B and Group C had different morphology. The aneurysm of Group C was more similar to human cerebral aneurysms but had a higher mortality rate than Group B. Group B was confirmed not only as an alternative method but also as a more safe and effective method for creating elastase-induced aneurysm models. Conclusion. Through analysis and comparison, the Group B is proven to be the simplest, reproducible, and most effective modeling method. The aneurysm model established by Group B can be used for basic research related to aneurysm mechanism. We have provided a new and effective method for basic research on aneurysm.

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“…The group proposed several modifications to their right CCA model to customize resultant aneurysm morphology. [31][32][33] Because the right CCA model is more of a sidewall-type aneurysm (origins as bifurcation from the subclavian artery), Ding et al 34 adapted their previous modifications to the left CCA (originates as a trifurcation between the brachiocephalic artery and aortic arch) to create a more bifurcation type elastase-induced aneurysm in 2013.…”

Section: Stump Aneurysm Modelmentioning

confidence: 99%

Preclinical extracranial aneurysm models for the study and treatment of brain aneurysms: A systematic review

Marbacher

Strange

Frösén

et al. 2020

J Cereb Blood Flow Metab

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Animal models make an important contribution to our basic understanding of the pathobiology of human brain aneurysms, are indispensable in testing novel treatment approaches, and are essential for training interventional neuroradiologists and neurosurgeons. Researchers are confronted with a broad diversity of models and techniques in various species. This systematic review aims to summarize and categorize extracranial aneurysm models and their characteristics, discuss advantages and disadvantages, and suggest the best use of each model. We searched the electronical Medline/PubMed database between 1950 and 2020 to identify main models and their refinements and technical modifications for creation of extracranial aneurysms. Each study included was assessed for aneurysm-specific characteristics, technical details of aneurysm creation, and histological findings. Among more than 4000 titles and abstracts screened, 473 studies underwent full-text analysis. From those, 68 different techniques/models in five different species were identified, analyzed in detail, and then grouped into one of the five main groups of experimental models as sidewall, terminal, stump, bifurcation, or complex aneurysm models. This systematic review provides a compact guide for investigators in selecting the most appropriate model from a range of techniques to best suit their experimental goals, practical considerations, and laboratory environment.

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Creation of Bifurcation-Type Elastase-Induced Aneurysms in Rabbits

Cited by 9 publications

References 13 publications

Four Surgical Modifications to the Classic Elastase Perfusion Aneurysm Model Enable Haemodynamic Alterations and Extended Elastase Perfusion

Four Surgical Modifications to the Classic Elastase Perfusion Aneurysm Model Enable Haemodynamic Alterations and Extended Elastase Perfusion

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

Preclinical extracranial aneurysm models for the study and treatment of brain aneurysms: A systematic review

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