Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Kato, Yuka; Iwata, Takanori; Washio, Kaoru; Yoshida, Toshiyuki; Kuroda, Hozue; Morikawa, Shunichi; Hamada, Mariko; Ikura, Kazuki; Kaibuchi, Nobuyuki; Yamato, Masayuki; Okano, Teruo; Uchigata, Yasuko

doi:10.3791/54539-v

Cited by 4 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These wounds are intractable and can significantly reduce patients' quality of life. [11] Diabetic wound care has developed into a distinct subspecialty, with clinicians frequently employing advanced treatments such as growth factors (GFs), stem cells, engineered skin, extracellular matrices (ECMs), and negative pressure wound therapy. [12,13] However, there is a lack of accessible evidence suggesting the efficiency of most current advanced wound treatment therapies.…”

Section: Introductionmentioning

confidence: 99%

Teicoplanin‐Decorated Reduced Graphene Oxide Incorporated Silk Protein Hybrid Hydrogel for Accelerating Infectious Diabetic Wound Healing and Preventing Diabetic Foot Osteomyelitis

Bakadia,

Zheng,

Qaed Ahmed

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Developing hybrid hydrogel dressings with anti‐inflammatory, antioxidant, angiogenetic, and antibiofilm activities with higher bone tissue penetrability to accelerate diabetic wound healing and prevent diabetic foot osteomyelitis (DFO) is highly desirable in managing diabetic wounds. Herein, the glycopeptide teicoplanin is used for the first time as a green reductant to chemically reduce graphene oxide (GO). The resulting teicoplanin‐decorated reduced graphene oxide (rGO) is incorporated into a mixture of silk proteins (SP) and crosslinked with genipin to yield a physicochemically crosslinked rGO‐SP hybrid hydrogel. This hybrid hydrogel exhibits high porosity, self‐healing, shear‐induced thinning, increased cell proliferation and migration, and mechanical properties suitable for tissue engineering. Moreover, the hybrid hydrogel eradicates bacterial biofilms with a high penetrability index in agar and hydroxyapatite discs covered with biofilms, mimicking bone tissue. In vivo, the hybrid hydrogel accelerates the healing of non‐infected wounds in a diabetic rat and infected wounds in a diabetic mouse by up‐regulating anti‐inflammatory cytokines and down‐regulating matrix metalloproteinase‐9, promoting M2 macrophage polarization and angiogenesis. The implantation of hybrid hydrogel into the infected site of mouse tibia improves bone regeneration. Hence, the rGO‐SP hybrid hydrogel can be a promising wound dressing for treating infectious diabetic wounds, providing a further advantage in preventing DFO.This article is protected by copyright. All rights reserved

show abstract

Section: Introductionmentioning

confidence: 99%

Teicoplanin‐Decorated Reduced Graphene Oxide Incorporated Silk Protein Hybrid Hydrogel for Accelerating Infectious Diabetic Wound Healing and Preventing Diabetic Foot Osteomyelitis

Bakadia,

Zheng,

Qaed Ahmed

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Therefore, ASCs have been considered potential treatments for promoting the healing of chronic wounds in DM models. In addition, many therapeutic strategies include the combination of ASCs and biomaterials [ [13] , [14] , [15] ]. However, methods for maintaining the quality of ASCs during transplantation are still under investigation.…”

Section: Introductionmentioning

confidence: 99%

Engineered elastin-like polypeptide improves the efficiency of adipose-derived stem cell-mediated cutaneous wound healing in type II diabetes mellitus

Woo,

Choi,

et al. 2023

Heliyon

View full text Add to dashboard Cite

Tissue Engineering and Regenerative Medicine in Craniofacial Reconstruction and Facial Aesthetics

Borrelli

Longaker

et al. 2020

Journal of Craniofacial Surgery

View full text Add to dashboard Cite

The craniofacial region is anatomically complex and is of critical functional and cosmetic importance, making reconstruction challenging. The limitations of current surgical options highlight the importance of developing new strategies to restore the form, function, and esthetics of missing or damaged soft tissue and skeletal tissue in the face and cranium. Regenerative medicine (RM) is an expanding field which combines the principles of tissue engineering (TE) and self-healing in the regeneration of cells, tissues, and organs, to restore their impaired function. RM offers many advantages over current treatments as tissue can be engineered for specific defects, using an unlimited supply of bioengineered resources, and does not require immunosuppression. In the craniofacial region, TE and RM are being increasingly used in preclinical and clinical studies to reconstruct bone, cartilage, soft tissue, nerves, and blood vessels. This review outlines the current progress that has been made toward the engineering of these tissues for craniofacial reconstruction and facial esthetics.

show abstract

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Cited by 4 publications

References 0 publications

Teicoplanin‐Decorated Reduced Graphene Oxide Incorporated Silk Protein Hybrid Hydrogel for Accelerating Infectious Diabetic Wound Healing and Preventing Diabetic Foot Osteomyelitis

Teicoplanin‐Decorated Reduced Graphene Oxide Incorporated Silk Protein Hybrid Hydrogel for Accelerating Infectious Diabetic Wound Healing and Preventing Diabetic Foot Osteomyelitis

Engineered elastin-like polypeptide improves the efficiency of adipose-derived stem cell-mediated cutaneous wound healing in type II diabetes mellitus

Tissue Engineering and Regenerative Medicine in Craniofacial Reconstruction and Facial Aesthetics

Contact Info

Product

Resources

About