Creation and testing of a new, local microtubule‐disruption tool based on the microtubule‐severing enzyme, katanin p60

Advani, Siddheshwari; Maresca, Thomas J.; Ross, Jennifer L.

doi:10.1002/cm.21482

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…While this is the case, elucidating the regulation partners of p60‐katanin will provide important data for understanding the functional regulation of both this protein and microtubule severing. While its transcriptional (Elk‐1, p53) (Kelle et al, 2019; Kırımtay et al, 2020; Selçuk et al, 2013) and posttranslational regulators (AURKB by phosphorylating, DYRK2 by ubiquitinating; Advani et al, 2018; Maddika & Chen, 2009) are studied in more detail, information on posttranscriptional regulators of katanin is very limited. At this point, microRNAs (miRNAs), a group of small (21–23 nucleotides) noncoding RNAs have recently drawn attention for their posttranscriptional regulatory function.…”

Section: Introductionmentioning

confidence: 99%

A potential posttranscriptional regulator for p60‐katanin: miR‐124‐3p

et al. 2023

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Katanin is a microtubule severing protein belonging to the ATPase family and consists of two subunits; p60‐katanin synthesized by the KATNA1 gene and p80‐katanin synthesized by the KATNB1 gene. Microtubule severing is one of the mechanisms that allow the reorganization of microtubules depending on cellular needs. While this reorganization of microtubules is associated with mitosis in dividing cells, it primarily takes part in the formation of structures such as axons and dendrites in nondividing mature neurons. Therefore, it is extremely important in neuronal branching. p60 and p80 katanin subunits coexist in the cell. While p60‐katanin is responsible for cutting microtubules with its ATPase function, p80‐katanin is responsible for the regulation of p60‐katanin and its localization in the centrosome. Although katanin has vital functions in the cell, there are no known posttranscriptional regulators of it. MicroRNAs (miRNAs) are a group of small noncoding ribonucleotides that have been found to have important roles in regulating gene expression posttranscriptionally. Despite being important in gene regulation, so far no microRNA has been experimentally associated with katanin regulation. In this study, the effects of miR‐124‐3p, which we detected as a result of bioinformatics analysis to have the potential to bind to the p60 katanin mRNA, were investigated. For this aim, in this study, SH‐SY5Y neuroblastoma cells were transfected with pre‐miR‐124‐3p mimics and pre‐mir miRNA precursor as a negative control, and the effect of this transfection on p60‐katanin expression was measured at both RNA and protein levels by quantitative real‐time PCR (qRT‐PCR) and western blotting, respectively. The results of this study showed for the first time that miR‐124‐3p, which was predicted to bind p60‐katanin mRNA by bioinformatic analysis, may regulate the expression of the KATNA1 gene. The data obtained within the scope of this study will make important contributions in order to better understand the regulation of the expression of p60‐katanin which as well will have an incontrovertible impact on the understanding of the importance of cytoskeletal reorganization in both mitotic and postmitotic cells.

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Section: Introductionmentioning

confidence: 99%

A potential posttranscriptional regulator for p60‐katanin: miR‐124‐3p

et al. 2023

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The Mammalian Family of Katanin Microtubule-Severing Enzymes

Lynn

Martinez

Nguyen

et al. 2021

Front. Cell Dev. Biol.

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The katanin family of microtubule-severing enzymes is critical for cytoskeletal rearrangements that affect key cellular processes like division, migration, signaling, and homeostasis. In humans, aberrant expression, or dysfunction of the katanins, is linked to developmental, proliferative, and neurodegenerative disorders. Here, we review current knowledge on the mammalian family of katanins, including an overview of evolutionary conservation, functional domain organization, and the mechanisms that regulate katanin activity. We assess the function of katanins in dividing and non-dividing cells and how their dysregulation promotes impaired ciliary signaling and defects in developmental programs (corticogenesis, gametogenesis, and neurodevelopment) and contributes to neurodegeneration and cancer. We conclude with perspectives on future katanin research that will advance our understanding of this exciting and dynamic class of disease-associated enzymes.

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Creation and testing of a new, local microtubule‐disruption tool based on the microtubule‐severing enzyme, katanin p60

Cited by 2 publications

References 40 publications

A potential posttranscriptional regulator for p60‐katanin: miR‐124‐3p

A potential posttranscriptional regulator for p60‐katanin: miR‐124‐3p

The Mammalian Family of Katanin Microtubule-Severing Enzymes

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