Creating de novo peptide-based bioactivities: from assembly to origami

Abstract

Chain-like peptides and DNA share obvious similarities in terms of chain hybridization; thus, replacing DNA with peptides to create bioactivities not only has high theoretical feasibility but also provides a new bottom-up synthetic strategy.

Messenger RNA-Based Therapeutics and Vaccines: What’s beyond COVID-19?

“…Peptides represent other versatile, biocompatible, and targetable RNA carriers. From this class, cell-penetrating peptides (CPPs) that can penetrate the cellular membrane and transfer to the cytoplasm are used most frequently for RNA delivery by forming a variety of nanocomplexes depending on the formulation settings and the properties of the used CPPs and RNAs. , …”

“…From this class, cell-penetrating peptides (CPPs) that can penetrate the cellular membrane and transfer to the cytoplasm are used most frequently for RNA delivery by forming a variety of nanocomplexes depending on the formulation settings and the properties of the used CPPs and RNAs. 81,82 A further novel biological alternative to lipid nanoparticle carriers is the use of cell-based delivery systems, exploiting the cellular paracrine communication ability to directly deliver proteins synthesized by mRNA brought into cells ex vivo. It provides certain advantages, such as biocompatibility, lack of toxicity, and extended half-life in circulation.…”