Creating Electrospun Nanofiber-Based Biomimetic Scaffolds for Bone Regeneration

Katsanevakis, Eleni; Wen, Xuejun; Zhang, Ning

doi:10.1007/12_2011_131

Cited by 7 publications

(3 citation statements)

References 157 publications

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“…In another, the partial phase separation of prolamin proteins increased the hydrophobic fillers and induced conformational change of proteins to form stronger hydrogen bonds, resulting in reinforced mechanical properties. With the addition of 3 wt % SCN, the tensile strength of hz-c3-a2 was further increased to 21.99 ± 1.19 and 15.78 ± 0.27 MPa, even higher than the mechanical properties of cancellous bones (strength of 5−10 MPa and modulus of 50−100 MPa), 50 suggesting the potential use as tissue scaffold. Therefore, both SCN and alignment had a tremendous effect on improving the mechanical properties of electrospun hordein/zein fibers.…”

Section: Resultsmentioning

confidence: 97%

Cellulose Nanowhiskers and Fiber Alignment Greatly Improve Mechanical Properties of Electrospun Prolamin Protein Fibers

Wang

Chen

2014

ACS Appl. Mater. Interfaces

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Electrospun fibers from natural polymers must possess appropriate mechanical properties if they are to be functional in numerous applications. In this research, two convenient physical approaches were applied to reinforce the assembled hordein/zein electrospun nanofabrics: incorporation of surface-modified cellulose nanowhiskers (SCN) and fiber alignment. The mechanical properties and stability of the modified fibers were tested in relation to fiber morphology and structure as characterized by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, and Raman spectroscopy. SCN modified by quaternary ammonium salt were well-dispersed in hordein/zein networks, leading to fibers with significantly improved mechanical properties and water resistance. With the addition of 3 wt % SCN, the tensile strength and Young's modulus of hordein/zein fibers increased from 4.36 ± 0.29 to 7.79 ± 0.36 MPa and from 195.80 ± 13.02 to 396.64 ± 18.33 MPa, respectively, and the elongation at break was retained because of the formation of a percolating network of SCN. The alignment of electrospun fibers strengthened the hordein/zein nanofabrics in both tangential and normal directions to 17.26 ± 1.41 and 14.02 ± 0.74 MPa, respectively, by not only altering the piling up pattern, but also by promoting phase separation and improved interactions. When applying both of the reinforcing methods, the tensile strength of hordein/zein fibers was further enhanced to 21.99 ± 1.19 MPa, stronger than that of cancellous bones (5-10 MPa). All the reinforced fibers exhibited a reduced burst effect in phosphate-buffered saline (PBS) while releasing the incorporated bioactive molecule in a controlled manner. These physically reinforced prolamin protein fibers possessed significantly improved mechanical properties and may have potential to be used as tissue engineering scaffold materials or natural delivery systems for biomedical applications.

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Section: Resultsmentioning

confidence: 97%

Cellulose Nanowhiskers and Fiber Alignment Greatly Improve Mechanical Properties of Electrospun Prolamin Protein Fibers

Wang

Chen

2014

ACS Appl. Mater. Interfaces

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“…The resultant scaffolds exhibited significantly higher proliferation and ALP activity after 7 days in culture compared to scaffolds produced by 3D printing alone. 72,73 Similarly, Yu et al 74 also combined electrospinning with 3D printing to produce 3D bone tissue scaffolds. However, they infused PCL/gelatin dispersed nanofibers into the meshes of PCL printing scaffold to fabricate 3D composite scaffolds (Figure 10).…”

Section: Combining Electrospinning With Other Processesmentioning

confidence: 99%

Methods of producing three dimensional electrospun scaffolds for bone tissue engineering: A review

Kareem

Tanner

2022

Proc Inst Mech Eng H

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Bone is a dynamic, living tissue that exists and renews itself continuously in a 3D manner. Nevertheless, complex clinical conditions require a bone substitute to replace the defective bone and/or accelerate bone healing. Bone tissue engineering aims to treat bone defects that fail to heal on their own. Electrospinning provides an opportunity to create nano- to micro-fibrous scaffolds that mimic the architecture of the natural extracellular matrix (ECM) with high porosity and large specific surface area. Despite these advantages, traditional electrospun meshes can only provide a 2D architecture for cell attachment and proliferation rather than the 3D attachment in native tissue. Fabrication of 3D electrospun scaffolds for bone tissue regeneration is a challenging task, which has attracted significant attention over the past couple of decades. This review highlights recent strategies used to produce 3D electrospun/co-electrospun scaffolds for bone tissue applications describing the materials and procedures. It also considers combining conventional and coaxial electrospinning with other scaffold manufacturing techniques to produce 3D structures which have the potential to engineer missing bone in the human body. Graphical abstract [Formula: see text]

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“…Critical parameters for designing membrane/scaffold in GBR technique include biocompatibility, biodegradability, optimal mechanical strength, and ability to regulate appropriate cellular activities. [13][14][15] An "ideal" degradable GBR polymeric membrane has yet to be found due to the disadvantage of polyester-based resorbable membranes (eg, poor cell response/weak osteoconductive or osteoinductive properties). To address this challenge, various approaches have been emerged to meet the demands of degradable GBR polymeric membranes in terms of mechanical properties, biocompatibility, and biological response, such as the following strategies: 1) Assembling multiple polymers (synthetic or natural) to maximize the advantages and minimize respective disadvantages.…”

Section: Introductionmentioning

confidence: 99%

The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

Qian¹,

Chen²,

Yang³

et al. 2016

IJN

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Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.

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Creating Electrospun Nanofiber-Based Biomimetic Scaffolds for Bone Regeneration

Cited by 7 publications

References 157 publications

Cellulose Nanowhiskers and Fiber Alignment Greatly Improve Mechanical Properties of Electrospun Prolamin Protein Fibers

Cellulose Nanowhiskers and Fiber Alignment Greatly Improve Mechanical Properties of Electrospun Prolamin Protein Fibers

Methods of producing three dimensional electrospun scaffolds for bone tissue engineering: A review

The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

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