Creating conductive structures for cell growth: Growth and alignment of myogenic cell types on polythiophenes

Breukers, Robert; Gilmore, Kerry J.; Kita, Magdalena; Wagner, Klaudia; Higgins, Michael J.; Moulton, Simon E.; Clark, Graeme M.; Officer, David L.; Kapsa, Robert M. I.; Wallace, Gordon G.

doi:10.1002/jbm.a.32822

Cited by 68 publications

(53 citation statements)

References 49 publications

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“…Alignment of organic micro-or nano-fibers is beneficial for enhanced charge transport [231,232,263,264], production of polarized light emission [265,266], improved absorption and photovoltaic properties [123,267], and enhanced crystal properties [268]. Additional benefits of parallel fiber arrangement include directional cell growth [269] and guided cell differentiation [270] as well as the achievement of high-modulus, highstrength fibers, which can be used for heat-resistant and protective clothing [271,272]. The multitude of applications requiring alignment makes evident the need for straightforward and uninvolved processes for producing aligned fiber structures beyond that of templated strategies.…”

Section: Es Fabrication Of Organic Micro-and Nano-fiber Devicesmentioning

confidence: 99%

Electrospinning for nano- to mesoscale photonic structures

et al. 2016

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Abstract:The fabrication of photonic and electronic structures and devices has directed the manufacturing industry for the last 50 years. Currently, the majority of small-scale photonic devices are created by traditional microfabrication techniques that create features by processes such as lithography and electron or ion beam direct writing. Microfabrication techniques are often expensive and slow. In contrast, the use of electrospinning (ES) in the fabrication of microand nano-scale devices for the manipulation of photons and electrons provides a relatively simple and economic viable alternative. ES involves the delivery of a polymer solution to a capillary held at a high voltage relative to the fiber deposition surface. Electrostatic force developed between the collection plate and the polymer promotes fiber deposition onto the collection plate. Issues with ES fabrication exist primarily due to an instability region that exists between the capillary and collection plate and is characterized by chaotic motion of the depositing polymer fiber. Material limitations to ES also exist; not all polymers of interest are amenable to the ES process due to process dependencies on molecular weight and chain entanglement or incompatibility with other polymers and overall process compatibility. Passive and active electronic and photonic fibers fabricated through the ES have great potential for use in light generation and collection in optical and electronic structures/ devices. ES produces fiber devices that can be combined with inorganic, metallic, biological, or organic materials for novel device design. Synergistic material selection and postprocessing techniques are also utilized for broad-ranging applications of organic nanofibers that span from biological to electronic, photovoltaic, or photonic. As the ability to electrospin optically and/or electronically active materials in a controlled manner continues to improve, the complexity and diversity of devices fabricated from this process can be expected to grow rapidly and provide an alternative to traditional resource-intensive fabrication techniques.

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Section: Es Fabrication Of Organic Micro-and Nano-fiber Devicesmentioning

confidence: 99%

Electrospinning for nano- to mesoscale photonic structures

et al. 2016

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“…In our previous investigations into the use of polythiophene substrates for the in vitro growth of skeletal muscle cells, we utilized two chemically prepared substituted polythiophenes of distinctly different surface characteristics [12]. It was demonstrated that, despite the diverse hydrophilic character of the films, both films supported the proliferation and differentiation of primary and transformed skeletal muscle myoblasts.…”

Section: Polymer Propertiesmentioning

confidence: 99%

“…A number of reports have highlighted the diversity of approaches that have been applied to improve skeletal muscle engineering, including the use of micro-patterned polymers [5][6][7], biological scaffolds [8] and synthetic polymers [9][10][11][12][13]. Synthetic scaffolds have the advantage over biologic scaffolds in that their characteristics can be defined and manipulated to be inherently pro-myogenic (i.e.…”

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confidence: 99%

“…Our group has recently investigated the use of an ester-functionalized polythiophene and its hydrolyzed derivative, demonstrating excellent capacity to support myoblast proliferation and differentiation in vitro [12]. In addition, this organic solvent-soluble polythiophene was electrospun to provide an aligned nanostructured scaffold that orientated myoblast differentiation, in vitro.…”

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In vitro growth and differentiation of primary myoblasts on thiophene based conducting polymers

Quigley¹,

Wagner²,

Kita³

et al. 2013

Biomater. Sci.

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Polythiophenes are attractive candidate polymers for use in synthetic cell scaffolds as they are amenable to modification of functional groups as a means by which to increase biocompatibility. In the current study we analysed the physical properties and response of primary myoblasts to three thiophene polymers synthesized from either a basic bithiophene monomer or from one of two different thiophene monomers with alkoxy functional groups. In addition, the effect of the dopants pTS-and ClO4 -was investigated. In general, it was found that pTS-doped polymers were significantly smoother and tended to be more hydrophilic than their ClO 4 -doped counterparts, demonstrating that the choice of dopant significantly affects the polythiophene physical properties. These properties had a significant effect on the response of primary myoblasts to the polymer surfaces; LDH activity measured from cells harvested at 24 and 48 h post-seeding revealed significant differences between numbers of cells attaching to the different thiophene polymers, whilst all of the polymers equally supported cell doubling over the 48 h period. Differences in morphology were also observed, with reduced cell spreading observed on polymers with alkoxy groups. In addition, significant differences were seen in the polymers' ability to support myoblast fusion. In general pTS-doped polymers were better able to support fusion than their ClO4 -doped counterparts. These studies demonstrate that modification of thiophene polymers can be used to promote specific cellular response (e.g. proliferation over differentiation) without the use of biological agents. 2013 The Royal Society of Chemistry. In vitro growth and differentiation of murine primary myoblasts on thiophene based conducting polymers. AbstractPolythiophenes are attractive candidate polymers for use in synthetic cell scaffolds as they are amenable to modification of functional groups as a means by which to increase biocompatibility.In the current study we analysed the physical properties and response of primary myoblasts to three thiophene polymers synthesized from either a basic bithiophene monomer or from one of two different thiophene monomers with alkoxy functional groups. In addition, the effect of the dopants pTS -and ClO 4 -was investigated. In general, it was found that pTS -doped polymers were significantly smoother and tended to be more hydrophilic than their ClO 4 -doped counterparts, demonstrating that the choice of dopant significantly affects the polythiophene physical properties.These properties had a significant effect on the response of primary myoblasts to the polymer surfaces; significant differences were seen in the rate of cell division, as determined by LDH activity. Differences in morphology were also observed, with reduced cell spreading observed on polymers with alkoxy groups. In addition, significant differences were seen in the polymers' ability to support myoblast fusion. In general pTS -doped polymers were better able to support fusion than their ClO 4 -doped co...

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“…Electrical and mechanical stimuli have demonstrated in the past to stimulate stem cells and to influence differentiation into cardiac type cells [9][10][11]. EAP coated fibres have been demonstrated to work as support for many types of cells, including neural, myogenic, and cardiac cells [12][13][14][15]. Following on from this foundation, we will produce EAP coated fibres using PPy to investigate the response from primary and stem cells.…”

Section: Introductionmentioning

confidence: 99%

Electroactive 3D materials for cardiac tissue engineering

et al. 2015

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Creating conductive structures for cell growth: Growth and alignment of myogenic cell types on polythiophenes

Cited by 68 publications

References 49 publications

Electrospinning for nano- to mesoscale photonic structures

Electrospinning for nano- to mesoscale photonic structures

In vitro growth and differentiation of primary myoblasts on thiophene based conducting polymers

Electroactive 3D materials for cardiac tissue engineering

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