Creating an efficient 1,2-dichloroethane-mineralizing bacterium by a combination of pathway engineering and promoter engineering

Influence of organic matters on the adsorption–desorption of 1,2-dichloroethane on soil in water and model saturated aquifer

“…The FA was prepared into aqueous solutions using deionized water, and HA was dissolved using 0.01 mol L −1 sodium hydroxide solution to ensure that the solute was almost completely dissolved and that the concentration remained stable over longer storage periods. The FA and HA solutions (1 : 1) were incorporated into the soil (original soil and soil treated with high temperature and oxidation) by diluting the stock solutions to selected concentrations (10,25,50, 75 mg L −1 ) and mixed with the soil. The mixtures were shaken for 24 hours at room temperature and then were dried at a constant temperature in an oven at 60 °C.…”

“…reported in previous studies. 7,25 The overall adsorption time is relatively long, which is inuenced by the altered conditions of the soil aer inltration. To ensure the adsorption equilibrium, the batch adsorption experiment was chosen to be carried out for 48 h.…”

Improvement in Salt Tolerance Ability of Pseudomonas putida KT2440

“…Pseudomonas putida KT2440, an ideal chassis for industrial biotechnology and bioremediation, has attracted increasing interest from researchers [ 39 , 40 , 41 , 42 ]. Recently, researchers make great efforts to reveal and elucidate the functional gene for tolerance under various stress conditions of P. putida KT2440 and to expand its biotechnological applications, such as a major facilitator superfamily transporter involved in propionic acid tolerance [ 43 ], an ABC transporters involved in furfural tolerance [ 44 ], and the efflux pump protein TtgB involved in p -coumaric and ferulic acid tolerance [ 45 ].…”

“…Recently, researchers make great efforts to reveal and elucidate the functional gene for tolerance under various stress conditions of P. putida KT2440 and to expand its biotechnological applications, such as a major facilitator superfamily transporter involved in propionic acid tolerance [ 43 ], an ABC transporters involved in furfural tolerance [ 44 ], and the efflux pump protein TtgB involved in p -coumaric and ferulic acid tolerance [ 45 ]. Some researchers engineered strain KT2440 for biodegradation of xenobiotic pollutains, like bioremediation of naphthalene [ 46 ], carbofuran and chlorpyrifos [ 47 ], and 1,2-dichloroethane [ 39 ]. The salt tolerance of strain KT2440 is a desirable phenotype for bioconversion and bioremediation, especially for bioremediation exposed to highly saline wastewater or saline oil [ 48 , 49 ].…”