2024
DOI: 10.1007/s11103-024-01445-w
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Creating a zero amylose barley with high soluble sugar content by genome editing

Yun Li,
Yanyan Jiang,
Dong Cao
et al.

Abstract: Amylose biosynthesis is strictly associated with granule-bound starch synthase I (GBSSI) encoded by the Waxy gene. Mutagenesis of single bases in the Waxy gene, which induced by CRISPR/Cas9 genome editing, caused absence of intact GBSSI protein in grain of the edited line. Consequently, B-type granules disappeared. The amylose and amylopectin contents of waxy mutants were zero and 31.73%, while those in the wild type were 33.50% and 39.00%, respectively. The absence of waxy protein led to increase in soluble s… Show more

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