Creatine supplementation increases soleus muscle creatine content and lowers the insulinogenic index in an animal model of inherited type 2 diabetes

Eijnde, Bert O.; Jijakli, Hassan; Hespel, Peter; Malaisse, Willy

doi:10.3892/ijmm.17.6.1077

Cited by 21 publications

(31 citation statements)

References 29 publications

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“…In a diabetes experimental model, it has been observed that creatine supplementation can improve the sensibility to insulin in extrapancreatic sites (25) . Other works which study the relationship between creatine use and glucose metabolism show that such supplementation, besides the expected benefits in the application in sports medicine, could have other applications.…”

Section: Discussionmentioning

confidence: 99%

Resistência à insulina com a suplementação de creatina em animais de experimentação

Costallat¹,

Miglioli²,

Silva³

et al. 2007

Rev Bras Med Esporte

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Introduction and objective:Creatine supplementation has been used in order to improve muscular performance. This substance affects glucose metabolism and stimulates the in vitro as well as the in vivo insulin secretion. Nevertheless, long-term insulin hypersecretion may also induce insulin resistance. The present work analyzed the effects of creatine oral supplementation in order to evaluate the possibility of occurrence of resistance to in vivo insulin. Methods: Forty-eight Wistar rats (24 female/ 24 male) were divided in two groups of 24 (control and study) and subdivided in six groups of eight. They were fed with standard food during four weeks, having water ad libitum. Moreover, the study group received dietetic supplement of creatine (0.4 g creatine for 30 ml of water per rat / day). In the 7 th , 14 th , 21 st and 28 th day of the experiment, 12 rats were anesthetized (sodium thiopental 0.15 mL/ 100 g) after six hour-fasting, being submitted to intravenous insulin tolerance test (0.5 mL of 30% regular human insulin and 70% saline solution). The blood samples were collected from the tail veins of the rats, in the basal, three, six, nine, 12 and 15 minutes after insulin administration times. The glucose measurement was performed through the glucose oxidase method. The study was previously approved by the Research Ethics Committee of CCMB-PUC-SP. Results: The mean of the glucose decrease constant (K ITT ) was calculated through the formula 0.693/ T 1/2 . The study group, when compared with the control group, presented insulin resistance at day 21 (p < 0.0004) and day 28 (p < 0.0001). Conclusion: This study shows that extended creatine supplementation may lead to insulin resistance. Besides that, it should be carefully used in individuals with glucose metabolism disturbances.

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Section: Discussionmentioning

confidence: 99%

Resistência à insulina com a suplementação de creatina em animais de experimentação

Costallat¹,

Miglioli²,

Silva³

et al. 2007

Rev Bras Med Esporte

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“…Essa possibilidade parece ser bastante plausível, já que alguns grupos, incluindo o nosso, demonstraram o efeito sinérgico da creatina ao exercício na captação de glicose (3,6,21) . Em suma, a suplementação de creatina não influenciou a captação periférica de glicose e os estoques de glicogênio muscular e hepático em ratos submetidos à atividade física por quatro e oito semanas.…”

Section: Discussionunclassified

Efeitos da suplementação de creatina na captação de glicose em ratos submetidos ao exercício físico

Freire

Gualano

Leme

et al. 2008

Rev Bras Med Esporte

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RESUMOEstudos recentes têm sugerido que a suplementação de creatina é capaz de modular a homeostase da glicose, aumentando sua captação pelos tecidos periféricos. O objetivo deste trabalho foi investigar o efeito da suplementação de creatina na tolerância à glicose e no conteúdo de glicogênio muscular e hepático em ratos submetidos ou não à atividade física por quatro e oito semanas. Ratos Wistar foram divididos em dois grupos: quatro e oito semanas de intervenção. Posteriormente, cada grupo foi subdividido em quatro subgrupos, de acordo com a ingestão do suplemento e o treinamento: controle cedentário, controle treinado, suplementado sedentário e suplementado treinado. Os animais tiveram livre acesso à água e ração; o grupo suplementado teve 2% de sua ração sob a forma de creatina monoidratada. Os grupos exercitados nadaram 40 minutos por dia, quatro dias por semana, com carga entre 2 e 5% do seu peso amarrado ao peito. Após quatro e oito semanas, o teste oral de tolerância à glicose e as dosagens de glicogênio muscular e hepático foram realizadas. Não foram observadas diferenças significativas entre os grupos no teste de tolerância oral à glicose e no conteúdo de glicogênio muscular e hepático. Este estudo mostrou que a suplementação de creatina não exerceu influência na tolerância à glicose nem nas concentrações de glicogênio em ratos submetidos ou não à atividade física por quatro ou oito semanas.Palavras-chave: àcido α metil guanidino acético, condicionamento físico, glicemia. ABSTRACTRecently, studies have suggested that creatine supplementation can modulate glucose homeostasis by increasing glucose uptake in peripheral tissues. The aim of this study was to investigate the effects of creatine supplementation on glucose tolerance, muscle and hepatic glycogen content in rats submitted or not to physical activity for four and eight weeks. Wistar rats were divided in two groups: four and eight weeks of intervention. Afterwards, each group was subdivided in four subgroups, according to supplement intake and exercise: Sedentary Control; Trained Control; Supplemented Sedentary; and Supplemented Trained. The animals had free access to water and chow and the supplemented groups had two % of their diet as creatine monohydrated. The exercise groups swam for 40 minutes a day, four days a week, with two to five % of their body weight attached to their chests. After four and eight weeks, oral glucose tolerance tests were performed and both hepatic and muscle glycogen were determined. No significant differences were observed between groups on glucose tolerance and glycogen content in muscle and hepatic tissue. This study shows that creatine supplementation does not influence neither glucose tolerance nor glycogen concentrations in rats submitted or not to physical activity for four and eight weeks.Keywords: glycine, N-(aminoiminomethyl)-N-methyl-, physical fitness, blood glucose. (1) . Recentemente, essa amina tem sido investigada como uma possível ferramenta capaz de modular o metabolismo dos carboidratos. Ferrante et ...

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“…Only Ferrante et al (2000) studied mice; the other studies used rats (Rooney et al 2002;Young and Young 2002;Ju et al 2005;Souza et al 2006;Op't Eijnde et al 2006;Freire et al 2008;Vaisy et al 2011;Eijnde et al 2001;Nicastro et al 2012;Araújo et al 2013). Eight studies investigated healthy animals (Young and Young 2002;Rooney et al 2002;Ju et al 2005;Souza et al 2006;Freire et al 2008;Eijnde et al 2001;Vaisy et al 2011;Araújo et al 2013), Ferrante et al (2000 used a transgenic mouse model of Huntington's Disease, Op't Eijnde et al (2006) studied Goto-Kakizaki rats (a animal model of inherited T2DM), and Nicastro et al (2012) used rats treated with dexamethasone. Four studies examined the effects of creatine supplementation associated with exercise training on glucose metabolism (Souza et al 2006;Freire et al 2008;Vaisy et al 2011;Araújo et al 2013), and seven studies The search strategy consists of two separate components and each component consists of the key words related to "creatine supplementation" and the key words related to "glucose metabolism" individually.…”

Section: Animal Studiesmentioning

confidence: 96%

“…One study did not mention the sample size . Six studies were controlled (Ferrante et al 2000;Eijnde et al 2001;Ju et al 2005;Souza et al 2006;Op't Eijnde et al 2006;Araújo et al 2013), and 5 were randomized and controlled (Young and Young 2002;Rooney et al 2002;Freire et al 2008;Vaisy et al 2011;Nicastro et al 2012). The median sample size was 63 animals (ranging from 8 to 72).…”

Section: Animal Studiesmentioning

confidence: 98%

“…Four studies examined the effects of creatine supplementation associated with exercise training on glucose metabolism (Souza et al 2006;Freire et al 2008;Vaisy et al 2011;Araújo et al 2013), and seven studies The search strategy consists of two separate components and each component consists of the key words related to "creatine supplementation" and the key words related to "glucose metabolism" individually. The key words in each component were linked using "OR" as a Boolean function, and the results of the two sections were combined by utilizing the "AND" Boolean in final search adopted creatine supplementation alone (Young and Young 2002;Rooney et al 2002;Ju et al 2005;Souza et al 2006;Op't Eijnde et al 2006;Eijnde et al 2001;Nicastro et al 2012). Studies that involved exercise training employed swimming (Souza et al 2006;Freire et al 2008;Vaisy et al 2011) or treadmill running (Araújo et al 2013).…”

Section: Animal Studiesmentioning

confidence: 99%

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Creatine supplementation and glycemic control: a systematic review

et al. 2016

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The focus of this review is the effects of creatine supplementation with or without exercise on glucose metabolism. A comprehensive examination of the past 16 years of study within the field provided a distillation of key data. Both in animal and human studies, creatine supplementation together with exercise training demonstrated greater beneficial effects on glucose metabolism; creatine supplementation itself demonstrated positive results in only a few of the studies. In the animal studies, the effects of creatine supplementation on glucose metabolism were even more distinct, and caution is needed in extrapolating these data to different species, especially to humans. Regarding human studies, considering the samples characteristics, the findings cannot be extrapolated to patients who have poorer glycemic control, are older, are on a different pharmacological treatment (e.g., exogenous insulin therapy) or are physically inactive. Thus, creatine supplementation is a possible nutritional therapy adjuvant with hypoglycemic effects, particularly when used in conjunction with exercise.

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Creatine supplementation increases soleus muscle creatine content and lowers the insulinogenic index in an animal model of inherited type 2 diabetes

Cited by 21 publications

References 29 publications

Resistência à insulina com a suplementação de creatina em animais de experimentação

Resistência à insulina com a suplementação de creatina em animais de experimentação

Efeitos da suplementação de creatina na captação de glicose em ratos submetidos ao exercício físico

Creatine supplementation and glycemic control: a systematic review

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