Creatine pretreatment prevents birth asphyxia–induced injury of the newborn spiny mouse kidney

Ellery, Stacey J.; Ireland, Z.; Kett, Michelle M; Snow, Rodney J.; Walker, David W.; Dickinson, Hayley

doi:10.1038/pr.2012.174

Cited by 39 publications

(43 citation statements)

References 32 publications

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“…Cr supplementation has been reported to have a protective effect in neurons, myoblasts, and cardiomyocytes in culture when the cells are subjected to hypoxic and increased oxidative stressors (Adcock et al 1999(Adcock et al , 2002Balestrino et al 2002;Caretti et al 2010;Santacruz et al 2015a;Sartini et al 2012). Similar positive results have been reported in studies of the effects of Cr supplementation in animal models of neonatal hypoxia (Allah Yar et al 2015;Ellery et al 2013), and in a study with human volunteers that evaluated the Fig. 1 Schematic representation of the proposed organization of the CrT protein in the membrane.…”

Section: Introductionsupporting

confidence: 69%

Structural correlates of the creatine transporter function regulation: the undiscovered country

Santacruz

Jacobs²

2016

Amino Acids

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Creatine (Cr) and phosphocreatine constitute an energy shuttle that links ATP production in mitochondria to subcellular locations of ATP consumption. Cells in tissues that are reliant on this energy shuttle, such as myocytes and neurons, appear to have very limited ability to synthesize creatine. Therefore, these cells depend on Cr uptake across the cell membrane by a specialized creatine transporter (CrT solute carrier SLC6A8) in order to maintain intracellular creatine levels. Cr supplementation has been shown to have a beneficial effect in numerous in vitro and in vivo models, particularly in cases of oxidative stress, and is also widely used by athletes as a performance enhancement nutraceutical. Intracellular creatine content is maintained within narrow limits. However, the physiological and cellular mechanisms that mediate Cr transport during health and disease (such as cardiac failure) are not understood. In this narrative mini-review, we summarize the last three decades of research on CrT structure, function and regulation.

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Section: Introductionsupporting

confidence: 69%

Structural correlates of the creatine transporter function regulation: the undiscovered country

Santacruz

Jacobs²

2016

Amino Acids

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“…While creatine supplementation had no effect on fetal weight in normal pregnancies, effects where fetal growth restriction is present are yet to be determined. Our studies showing the protective effects of this creatine treatment on brain structure [20], postnatal behaviour [83], diaphragm [18], and kidney structure and function [19] following asphyxial birth in the spiny mouse gives reason to think that creatine may also benefit the preterm infants in their premature transition from fetal to newborn life.…”

Section: Reviewmentioning

confidence: 99%

Creatine supplementation during pregnancy: summary of experimental studies suggesting a treatment to improve fetal and neonatal morbidity and reduce mortality in high-risk human pregnancy

Dickinson

Ellery

Ireland

et al. 2014

BMC Pregnancy Childbirth

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While the use of creatine in human pregnancy is yet to be fully evaluated, its long-term use in healthy adults appears to be safe, and its well documented neuroprotective properties have recently been extended by demonstrations that creatine improves cognitive function in normal and elderly people, and motor skills in sleep-deprived subjects. Creatine has many actions likely to benefit the fetus and newborn, because pregnancy is a state of heightened metabolic activity, and the placenta is a key source of free radicals of oxygen and nitrogen. The multiple benefits of supplementary creatine arise from the fact that the creatine-phosphocreatine [PCr] system has physiologically important roles that include maintenance of intracellular ATP and acid–base balance, post-ischaemic recovery of protein synthesis, cerebral vasodilation, antioxidant actions, and stabilisation of lipid membranes. In the brain, creatine not only reduces lipid peroxidation and improves cerebral perfusion, its interaction with the benzodiazepine site of the GABAA receptor is likely to counteract the effects of glutamate excitotoxicity – actions that may protect the preterm and term fetal brain from the effects of birth hypoxia. In this review we discuss the development of creatine synthesis during fetal life, the transfer of creatine from mother to fetus, and propose that creatine supplementation during pregnancy may have benefits for the fetus and neonate whenever oxidative stress or feto-placental hypoxia arise, as in cases of fetal growth restriction, premature birth, or when parturition is delayed or complicated by oxygen deprivation of the newborn.

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“…One member of our research group has used this resource to successfully design quantitative real-time polymerase chain reaction (qPCR) primer sets for lung-specific genes, markers of hypoxia, inflammation and apoptosis, and nuclear coding genes associated with mitogenesis (n = 41; personal communication). Sanger sequencing of PCR products confirmed >85% success under standard qPCR conditions, which is a significant improvement compared to ~40% success rate reported for primer design based on homologous regions from human, mouse (Mus musculus) and rat (Rattus norvegicus) [33][34][35] . Another collaborator requested the sequence of the spiny mouse protein-coding Beta Amyloid transcript, the product of which is implicated in the etiology of Alzheimer's disease.…”

Section: Discussionmentioning

confidence: 73%

“…SOAPdenovo-Trans parameters: "max_rd_len = 150, rd_len_cutof = 150, avg_ins = 192, reverse_seq = 0, asm_flags = 3" with kmer lengths: 21,23,25,27,29,31,35,41,51,61,71,81,91. Trinity was used at kmer length 25, with parameters: "--normalize_reads --seqType fq --JM 100 G --CPU 20 --min_kmer_cov 2".…”

Section: Methodsmentioning

confidence: 99%

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De novotranscriptome assembly for the spiny mouse (Acomys cahirinus)

Mamrot

Legaie

Ellery

et al. 2016

Preprint

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Spiny mice of the genus Acomys display several unique physiological traits, including menstruation and scar-free wound healing; characteristics that are exceedingly rare in mammals, and of considerable interest to the scientific community. These unique attributes, and the potential for spiny mice to accurately model human diseases, are driving increased use of this genus in biomedical research, however little genetic information is accessible for this species. This project aimed to generate a draft transcriptome for the Common spiny mouse (Acomys cahirinus). Illumina sequencing of RNA from 15 organ types (male and female) produced 451 million, 150 bp paired-end reads (92.4Gbp). An extensive survey of de novo transcriptome assembly approaches using Trinity, SOAPdenovo-Trans, and Oases at multiple kmer lengths was conducted, producing 50 single-kmer assemblies from this dataset. Nonredundant transcripts from all assemblies were merged into a meta-assembly using the EvidentialGene tr2aacds pipeline, producing the largest gene catalogue to date for Acomys cahirinus. This study provides the first detailed characterization of the spiny mouse transcriptome. It validates use of the EvidentialGene tr2aacds pipeline in mammals to augment conventional de novo assembly approaches, and provides a valuable scientific resource for further investigation into the unique physiological characteristics inherent in the genus Acomys.The Common or Cairo spiny mouse (Acomys cahirinus) is a small rodent species endemic to the semi-arid deserts of Africa and the Middle East . Used in research to model human disease, spiny mice exhibit physiological characteristics not typically found in rodents: they exhibit a precocial pattern of development 2, 3 , atypical synthesis of hormones such as cortisol and dehydroepiandosterone [4][5][6] , and a menstrual cycle 7 . These traits are common to humans and other higher order primates, but rare in other mammals. For example, menstruation has been identified in only six non-primate species (from >5,000 extant mammals), none of which are rodents 8 . The discovery of human-like physiological characteristics in a rodent is highly valuable for those in the scientific community looking to model human conditions, however fundamental aspects of their biology remain unexplored; for instance, there is little genetic information accessible for this species.Publically available genetic information for the spiny mouse consists of the mitochondrial genome 9 , and two RNA sequencing (RNA-Seq) datasets: PRJNA184055 10 , and PRJNA292021 11 . These next-generation sequencing (NGS) datasets were created with specific aims: to establish incipient sympatric speciation as a mode of natural selection in mammals inhabiting divergent microclimates 9 , to examine the molecular basis for natural variation in mammalian lifespan 10 , and to characterize and investigate another characteristic unique to Acomys: scar-free wound healing and skin regeneration 11 . De novo assembly of NGS reads was conducted for each specific organ/...

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Creatine pretreatment prevents birth asphyxia–induced injury of the newborn spiny mouse kidney

Cited by 39 publications

References 32 publications

Structural correlates of the creatine transporter function regulation: the undiscovered country

Structural correlates of the creatine transporter function regulation: the undiscovered country

Creatine supplementation during pregnancy: summary of experimental studies suggesting a treatment to improve fetal and neonatal morbidity and reduce mortality in high-risk human pregnancy

De novotranscriptome assembly for the spiny mouse (Acomys cahirinus)

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