Creatine phosphokinase level in neuromuscular disorders effect of dilution and dialysis

Snehalatha, C.; Valmikinathan, K.; Srinivas, K.; Jagannathan, Kanchana

doi:10.1016/0009-8981(73)90385-9

Cited by 14 publications

(1 citation statement)

References 6 publications

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“…In preliminary experiments we tested several other CK assay procedures, including modifications of the widely used coupled enzyme method (Rosalki, 1967). Although these techniques are more sensitive than the phosphorus assay, they were developed for serum samples and are subject to interference with tissue substances, probably enzymes, that can not be completely removed by dialysis or dilution (Snehalatha et al, 1973). The present assay gives constant values over a wide range of sample dilution.…”

Section: Creatine Kinase Assaymentioning

confidence: 96%

Enhancement of skeletal muscle regeneration

Bischoff

Heintz

1994

Developmental Dynamics

161

103

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We have studied the effect of adding extra satellite cells or soluble factors from crushed muscle on regeneration of minced fragments from rat tibialis muscle. The muscle mince was wrapped in an artificial epimysium to prevent adhesions and cell immigration from adjacent muscles. Regeneration was quantitatively assessed by electrophoretic determination of the muscle-specific form of creatine kinase. Control minces exhibited three periods of change in creatine kinase activity during a 7-week regeneration period. Activity fell rapidly during the first week, then rose gradually from 1 3 weeks and increased more rapidly from 3-7 weeks. To augment the original complement of myogenic cells, satellite cells were isolated from the contralateral muscle, purified by density gradient centrifugation, and expanded in culture for 3 days before adding to the muscle mince. The added cells resulted in a 3-fold enhancement of creatine kinase activity throughout the regeneration period. Soluble muscle extract incorporated into a collagen matrix also stimulated regeneration when added to muscle mince. The extract accelerated the rate of creatine kinase increase during the 1 3 week period beyond that observed in the control or cell augmented mince, suggesting that factors in the extract may facilitate revascularization or reinnervation. The specific activity of creatine kinase was increased in regenerates augmented with both cells and extract, indicating that the effects enhance primarily myogenic processes.

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Section: Creatine Kinase Assaymentioning

confidence: 96%